Silver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action

Amber Nagy1, Alistair Harrison2, Supriya Sabbani3, Robert S Munson, Jr2, Prabir K Dutta3, W James Waldman11Department of Pathology, The Ohio State University; 2Center for Microbial Pathogenesis, Research Institute at Nationwide Children&#39;s Hospital, 3Department of Chemistry, The Ohio Stat...

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Autores principales:	Nagy A, Harrison A, Sabbani S, Munson,Jr. RS, Dutta PK, Waldman WJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c13a2f3ce5c64dd38a3a1897326e5a31
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spelling	oai:doaj.org-article:c13a2f3ce5c64dd38a3a1897326e5a312021-12-02T01:32:22ZSilver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action1176-91141178-2013https://doaj.org/article/c13a2f3ce5c64dd38a3a1897326e5a312011-09-01T00:00:00Zhttp://www.dovepress.com/silver-nanoparticles-embedded-in-zeolite-membranes-release-of-silver-i-a8217https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Amber Nagy1, Alistair Harrison2, Supriya Sabbani3, Robert S Munson, Jr2, Prabir K Dutta3, W James Waldman11Department of Pathology, The Ohio State University; 2Center for Microbial Pathogenesis, Research Institute at Nationwide Children&#39;s Hospital, 3Department of Chemistry, The Ohio State University, Columbus, OH, USABackground: The focus of this study is on the antibacterial properties of silver nanoparticles embedded within a zeolite membrane (AgNP-ZM).Methods and Results: These membranes were effective in killing Escherichia coli and were bacteriostatic against methicillin-resistant Staphylococcus aureus. E. coli suspended in Luria Bertani (LB) broth and isolated from physical contact with the membrane were also killed. Elemental analysis indicated slow release of Ag+ from the AgNP-ZM into the LB broth. The E. coli killing efficiency of AgNP-ZM was found to decrease with repeated use, and this was correlated with decreased release of silver ions with each use of the support. Gene expression microarrays revealed upregulation of several antioxidant genes as well as genes coding for metal transport, metal reduction, and ATPase pumps in response to silver ions released from AgNP-ZM. Gene expression of iron transporters was reduced, and increased expression of ferrochelatase was observed. In addition, upregulation of multiple antibiotic resistance genes was demonstrated. The expression levels of multicopper oxidase, glutaredoxin, and thioredoxin decreased with each support use, reflecting the lower amounts of Ag+ released from the membrane. The antibacterial mechanism of AgNP-ZM is proposed to be related to the exhaustion of antioxidant capacity.Conclusion: These results indicate that AgNP-ZM provide a novel matrix for gradual release of Ag+.Keywords: silver nanoparticles, zeolite, antibacterial agent, oxidative stressNagy AHarrison ASabbani SMunson,Jr. RSDutta PKWaldman WJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1833-1852 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Nagy A Harrison A Sabbani S Munson,Jr. RS Dutta PK Waldman WJ Silver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action
description	Amber Nagy1, Alistair Harrison2, Supriya Sabbani3, Robert S Munson, Jr2, Prabir K Dutta3, W James Waldman11Department of Pathology, The Ohio State University; 2Center for Microbial Pathogenesis, Research Institute at Nationwide Children&#39;s Hospital, 3Department of Chemistry, The Ohio State University, Columbus, OH, USABackground: The focus of this study is on the antibacterial properties of silver nanoparticles embedded within a zeolite membrane (AgNP-ZM).Methods and Results: These membranes were effective in killing Escherichia coli and were bacteriostatic against methicillin-resistant Staphylococcus aureus. E. coli suspended in Luria Bertani (LB) broth and isolated from physical contact with the membrane were also killed. Elemental analysis indicated slow release of Ag+ from the AgNP-ZM into the LB broth. The E. coli killing efficiency of AgNP-ZM was found to decrease with repeated use, and this was correlated with decreased release of silver ions with each use of the support. Gene expression microarrays revealed upregulation of several antioxidant genes as well as genes coding for metal transport, metal reduction, and ATPase pumps in response to silver ions released from AgNP-ZM. Gene expression of iron transporters was reduced, and increased expression of ferrochelatase was observed. In addition, upregulation of multiple antibiotic resistance genes was demonstrated. The expression levels of multicopper oxidase, glutaredoxin, and thioredoxin decreased with each support use, reflecting the lower amounts of Ag+ released from the membrane. The antibacterial mechanism of AgNP-ZM is proposed to be related to the exhaustion of antioxidant capacity.Conclusion: These results indicate that AgNP-ZM provide a novel matrix for gradual release of Ag+.Keywords: silver nanoparticles, zeolite, antibacterial agent, oxidative stress
format	article
author	Nagy A Harrison A Sabbani S Munson,Jr. RS Dutta PK Waldman WJ
author_facet	Nagy A Harrison A Sabbani S Munson,Jr. RS Dutta PK Waldman WJ
author_sort	Nagy A
title	Silver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action
title_short	Silver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action
title_full	Silver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action
title_fullStr	Silver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action
title_full_unstemmed	Silver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action
title_sort	silver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/c13a2f3ce5c64dd38a3a1897326e5a31
work_keys_str_mv	AT nagya silvernanoparticlesembeddedinzeolitemembranesreleaseofsilverionsandmechanismofantibacterialaction AT harrisona silvernanoparticlesembeddedinzeolitemembranesreleaseofsilverionsandmechanismofantibacterialaction AT sabbanis silvernanoparticlesembeddedinzeolitemembranesreleaseofsilverionsandmechanismofantibacterialaction AT munsonjrrs silvernanoparticlesembeddedinzeolitemembranesreleaseofsilverionsandmechanismofantibacterialaction AT duttapk silvernanoparticlesembeddedinzeolitemembranesreleaseofsilverionsandmechanismofantibacterialaction AT waldmanwj silvernanoparticlesembeddedinzeolitemembranesreleaseofsilverionsandmechanismofantibacterialaction
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Silver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action

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