Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora

Pulsatilla chinensis (Bunge) Regel is a commonly used Chinese medicine for clearing away heat and detoxification, cooling blood, stopping dysentery, and anti-inflammatory effects. Pulsatilla chinensis saponins (PRS) have been identified to be responsible for producing these pharmacological activitie...

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Autores principales:	Yali Liu, Mingyue Zhou, Ming Yang, Chen Jin, Yonggui Song, Jingbin Chen, Meng Gao, Zhifu Ai, Dan Su
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Pulsatilla chinensis saponins (PRS) 16S rRNA gut microbiome ulcerative colitis (UC) dextran sulfate sodium (DSS) Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c143199585ee48f5ac8395177b9a4907
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spelling	oai:doaj.org-article:c143199585ee48f5ac8395177b9a49072021-11-05T13:13:50ZPulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora2235-298810.3389/fcimb.2021.728929https://doaj.org/article/c143199585ee48f5ac8395177b9a49072021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.728929/fullhttps://doaj.org/toc/2235-2988Pulsatilla chinensis (Bunge) Regel is a commonly used Chinese medicine for clearing away heat and detoxification, cooling blood, stopping dysentery, and anti-inflammatory effects. Pulsatilla chinensis saponins (PRS) have been identified to be responsible for producing these pharmacological activities. Studies have shown that Pulsatilla decoction has a good therapeutic effect on ulcerative colitis (UC), however, the therapeutic effect of PRS on UC has not been reported. Therefore, the purpose of this study was to investigate the possible anti-UC activity of PRS using a dextran sulfate sodium (DSS)-induced rat model, and further study the mechanism of PRS in the treatment of UC. The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics. The results showed that PRS significantly reduced the mesenteric blood flow in UC rats and significantly alleviated the inflammatory response, which indicates that saponins are involved in the anti-UC effects of PRS. At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.Yali LiuYali LiuMingyue ZhouMing YangChen JinYonggui SongJingbin ChenMeng GaoZhifu AiDan SuFrontiers Media S.A.articlePulsatilla chinensis saponins (PRS)16S rRNAgut microbiomeulcerative colitis (UC)dextran sulfate sodium (DSS)MicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pulsatilla chinensis saponins (PRS) 16S rRNA gut microbiome ulcerative colitis (UC) dextran sulfate sodium (DSS) Microbiology QR1-502
spellingShingle	Pulsatilla chinensis saponins (PRS) 16S rRNA gut microbiome ulcerative colitis (UC) dextran sulfate sodium (DSS) Microbiology QR1-502 Yali Liu Yali Liu Mingyue Zhou Ming Yang Chen Jin Yonggui Song Jingbin Chen Meng Gao Zhifu Ai Dan Su Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora
description	Pulsatilla chinensis (Bunge) Regel is a commonly used Chinese medicine for clearing away heat and detoxification, cooling blood, stopping dysentery, and anti-inflammatory effects. Pulsatilla chinensis saponins (PRS) have been identified to be responsible for producing these pharmacological activities. Studies have shown that Pulsatilla decoction has a good therapeutic effect on ulcerative colitis (UC), however, the therapeutic effect of PRS on UC has not been reported. Therefore, the purpose of this study was to investigate the possible anti-UC activity of PRS using a dextran sulfate sodium (DSS)-induced rat model, and further study the mechanism of PRS in the treatment of UC. The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics. The results showed that PRS significantly reduced the mesenteric blood flow in UC rats and significantly alleviated the inflammatory response, which indicates that saponins are involved in the anti-UC effects of PRS. At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.
format	article
author	Yali Liu Yali Liu Mingyue Zhou Ming Yang Chen Jin Yonggui Song Jingbin Chen Meng Gao Zhifu Ai Dan Su
author_facet	Yali Liu Yali Liu Mingyue Zhou Ming Yang Chen Jin Yonggui Song Jingbin Chen Meng Gao Zhifu Ai Dan Su
author_sort	Yali Liu
title	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora
title_short	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora
title_full	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora
title_fullStr	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora
title_full_unstemmed	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora
title_sort	pulsatilla chinensis saponins ameliorate inflammation and dss-induced ulcerative colitis in rats by regulating the composition and diversity of intestinal flora
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c143199585ee48f5ac8395177b9a4907
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Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora

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