GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions

Soil is contaminated with salinity, which inhibits plant growth and development and reduces crop yields. The DREB (dehydration responsive element binding protein) gene responds to salt stresses through enhanced transcriptional expression and activation of genes involved in plant salinity resistance....

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Autores principales:	Tan Quang Tu, Phutthakone Vaciaxa, Thu Thi Mai Lo, Nhung Hong Nguyen, Nhan Thi Thanh Pham, Quan Huu Nguyen, Phat Tien Do, Lan Thi Ngoc Nguyen, Yen Thi Hai Nguyen, Mau Hoang Chu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	GmDREB6 NtP5CS NtCLC Salt tolerance Transcription levels Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c1474cf0d408421cabcb158e09edb1ae
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spelling	oai:doaj.org-article:c1474cf0d408421cabcb158e09edb1ae2021-11-20T04:56:50ZGmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions1319-562X10.1016/j.sjbs.2021.08.018https://doaj.org/article/c1474cf0d408421cabcb158e09edb1ae2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006896https://doaj.org/toc/1319-562XSoil is contaminated with salinity, which inhibits plant growth and development and reduces crop yields. The DREB (dehydration responsive element binding protein) gene responds to salt stresses through enhanced transcriptional expression and activation of genes involved in plant salinity resistance. In this study, we present the results of the analysis of the expression of the GmDREB6 transgene, a gene that encodes the soybean DREB6 transcription factor, regulating the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions. The transcription of GmDREB6, NtP5CS, and NtCLC in transgenic tobacco lines was confirmed by qRT-PCR. Under salt stress conditions, the GmDREB6 gene transcription levels in the transgenic tobacco lines L1 and L9 had increased from 2.40- to 3.22- fold compared with the condition without salinity treatment. Two transgenic lines, L1 and L9, had transcription levels of the P5CS gene that had increased from 1.24- to 3.60- fold compared with WT plants. For the NtCLC gene, under salt stress conditions, the transgenic lines had transcription levels that had increased by 3.65–4.54 (fold) compared with WT plants (P < 0.05). The L1-transgenic tobacco line showed simultaneous expression of both the GmDREB6 transgene and two intrinsic genes, the NtP5CS and NtCLC genes. This study demonstrated that expression of the GmDREB6 gene from soybean increases the transcription levels of the NtP5CS and NtCLC genes in transgenic tobacco plants under salt stress conditions. The analysis results have suggested that the GmDREB6 gene is a potential candidate for improving the salt tolerance of plants, opening up research and development opportunities for salt stress-tolerant crops to respond to climate change and the rise in sea levels.Tan Quang TuPhutthakone VaciaxaThu Thi Mai LoNhung Hong NguyenNhan Thi Thanh PhamQuan Huu NguyenPhat Tien DoLan Thi Ngoc NguyenYen Thi Hai NguyenMau Hoang ChuElsevierarticleGmDREB6NtP5CSNtCLCSalt toleranceTranscription levelsBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7175-7181 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	GmDREB6 NtP5CS NtCLC Salt tolerance Transcription levels Biology (General) QH301-705.5
spellingShingle	GmDREB6 NtP5CS NtCLC Salt tolerance Transcription levels Biology (General) QH301-705.5 Tan Quang Tu Phutthakone Vaciaxa Thu Thi Mai Lo Nhung Hong Nguyen Nhan Thi Thanh Pham Quan Huu Nguyen Phat Tien Do Lan Thi Ngoc Nguyen Yen Thi Hai Nguyen Mau Hoang Chu GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions
description	Soil is contaminated with salinity, which inhibits plant growth and development and reduces crop yields. The DREB (dehydration responsive element binding protein) gene responds to salt stresses through enhanced transcriptional expression and activation of genes involved in plant salinity resistance. In this study, we present the results of the analysis of the expression of the GmDREB6 transgene, a gene that encodes the soybean DREB6 transcription factor, regulating the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions. The transcription of GmDREB6, NtP5CS, and NtCLC in transgenic tobacco lines was confirmed by qRT-PCR. Under salt stress conditions, the GmDREB6 gene transcription levels in the transgenic tobacco lines L1 and L9 had increased from 2.40- to 3.22- fold compared with the condition without salinity treatment. Two transgenic lines, L1 and L9, had transcription levels of the P5CS gene that had increased from 1.24- to 3.60- fold compared with WT plants. For the NtCLC gene, under salt stress conditions, the transgenic lines had transcription levels that had increased by 3.65–4.54 (fold) compared with WT plants (P < 0.05). The L1-transgenic tobacco line showed simultaneous expression of both the GmDREB6 transgene and two intrinsic genes, the NtP5CS and NtCLC genes. This study demonstrated that expression of the GmDREB6 gene from soybean increases the transcription levels of the NtP5CS and NtCLC genes in transgenic tobacco plants under salt stress conditions. The analysis results have suggested that the GmDREB6 gene is a potential candidate for improving the salt tolerance of plants, opening up research and development opportunities for salt stress-tolerant crops to respond to climate change and the rise in sea levels.
format	article
author	Tan Quang Tu Phutthakone Vaciaxa Thu Thi Mai Lo Nhung Hong Nguyen Nhan Thi Thanh Pham Quan Huu Nguyen Phat Tien Do Lan Thi Ngoc Nguyen Yen Thi Hai Nguyen Mau Hoang Chu
author_facet	Tan Quang Tu Phutthakone Vaciaxa Thu Thi Mai Lo Nhung Hong Nguyen Nhan Thi Thanh Pham Quan Huu Nguyen Phat Tien Do Lan Thi Ngoc Nguyen Yen Thi Hai Nguyen Mau Hoang Chu
author_sort	Tan Quang Tu
title	GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions
title_short	GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions
title_full	GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions
title_fullStr	GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions
title_full_unstemmed	GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions
title_sort	gmdreb6, a soybean transcription factor, notably affects the transcription of the ntp5cs and ntclc genes in transgenic tobacco under salt stress conditions
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c1474cf0d408421cabcb158e09edb1ae
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GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions

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