Nucleolar protein 6 promotes cell proliferation and acts as a potential novel prognostic marker for hepatocellular carcinoma

Abstract. Background:. Nucleolar protein 6 (NOL6) is a nucleolar RNA-associated protein that is highly conserved between species. It has been proved to be associated with the prognosis of liver cancer. However, the underlying mechanism has not been fully established. This study aimed to assess the r...

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Autores principales:	Lei Meng, Kai-Xuan Xu, Ming-Xi Zhao, Kang Li, Kun Zhu, Da-Wei Yuan, Hao-Nan Wang, Peng-Gao Dai, Rong Yan, Jing Ni
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/c147624976414902a3d5178e959c5a80
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spelling	oai:doaj.org-article:c147624976414902a3d5178e959c5a802021-11-25T07:57:20ZNucleolar protein 6 promotes cell proliferation and acts as a potential novel prognostic marker for hepatocellular carcinoma0366-69992542-564110.1097/CM9.0000000000001655https://doaj.org/article/c147624976414902a3d5178e959c5a802021-11-01T00:00:00Zhttp://journals.lww.com/10.1097/CM9.0000000000001655https://doaj.org/toc/0366-6999https://doaj.org/toc/2542-5641Abstract. Background:. Nucleolar protein 6 (NOL6) is a nucleolar RNA-associated protein that is highly conserved between species. It has been proved to be associated with the prognosis of liver cancer. However, the underlying mechanism has not been fully established. This study aimed to assess the relationship between NOL6 and liver cancer prognosis. Methods:. We constructed an NOL6-short hairpin RNA (shRNA)-expressing lentivirus. Through viral transfection, cell growth assay and fluorescence-activated cell sorting, we evaluated the effect of shRNA-mediated NOL6 knockdown on the proliferation, colony formation, and apoptosis of hepatocellular carcinoma (HCC) cells. The relationship between NOL6 expression and HCC patient survival has been established through bioinformatics analysis. We also explored the downstream molecular regulatory network of NOL6 in HCC by performing an Ingenuity Pathway Analysis in the database. Results:. Increased NOL6 expression was detected in HCC cells compared to normal controls; HCC patients with high NOL6 expression had poorer prognoses than those with low expression. NOL6 knockdown inhibited HCC cell proliferation, apoptosis, and colony formation. Also, MAPK8, CEBPA, and FOSL1 were selected as potential downstream genes of NOL6. Conclusions:. NOL6 up-regulates HCC cell proliferation and affects downstream expression of related genes. Moreover, NOL6 is considered to be associated with poor prognosis in HCC patients.Lei MengKai-Xuan XuMing-Xi ZhaoKang LiKun ZhuDa-Wei YuanHao-Nan WangPeng-Gao DaiRong YanJing NiWolters KluwerarticleMedicineRENChinese Medical Journal, Vol 134, Iss 21, Pp 2611-2618 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Lei Meng Kai-Xuan Xu Ming-Xi Zhao Kang Li Kun Zhu Da-Wei Yuan Hao-Nan Wang Peng-Gao Dai Rong Yan Jing Ni Nucleolar protein 6 promotes cell proliferation and acts as a potential novel prognostic marker for hepatocellular carcinoma
description	Abstract. Background:. Nucleolar protein 6 (NOL6) is a nucleolar RNA-associated protein that is highly conserved between species. It has been proved to be associated with the prognosis of liver cancer. However, the underlying mechanism has not been fully established. This study aimed to assess the relationship between NOL6 and liver cancer prognosis. Methods:. We constructed an NOL6-short hairpin RNA (shRNA)-expressing lentivirus. Through viral transfection, cell growth assay and fluorescence-activated cell sorting, we evaluated the effect of shRNA-mediated NOL6 knockdown on the proliferation, colony formation, and apoptosis of hepatocellular carcinoma (HCC) cells. The relationship between NOL6 expression and HCC patient survival has been established through bioinformatics analysis. We also explored the downstream molecular regulatory network of NOL6 in HCC by performing an Ingenuity Pathway Analysis in the database. Results:. Increased NOL6 expression was detected in HCC cells compared to normal controls; HCC patients with high NOL6 expression had poorer prognoses than those with low expression. NOL6 knockdown inhibited HCC cell proliferation, apoptosis, and colony formation. Also, MAPK8, CEBPA, and FOSL1 were selected as potential downstream genes of NOL6. Conclusions:. NOL6 up-regulates HCC cell proliferation and affects downstream expression of related genes. Moreover, NOL6 is considered to be associated with poor prognosis in HCC patients.
format	article
author	Lei Meng Kai-Xuan Xu Ming-Xi Zhao Kang Li Kun Zhu Da-Wei Yuan Hao-Nan Wang Peng-Gao Dai Rong Yan Jing Ni
author_facet	Lei Meng Kai-Xuan Xu Ming-Xi Zhao Kang Li Kun Zhu Da-Wei Yuan Hao-Nan Wang Peng-Gao Dai Rong Yan Jing Ni
author_sort	Lei Meng
title	Nucleolar protein 6 promotes cell proliferation and acts as a potential novel prognostic marker for hepatocellular carcinoma
title_short	Nucleolar protein 6 promotes cell proliferation and acts as a potential novel prognostic marker for hepatocellular carcinoma
title_full	Nucleolar protein 6 promotes cell proliferation and acts as a potential novel prognostic marker for hepatocellular carcinoma
title_fullStr	Nucleolar protein 6 promotes cell proliferation and acts as a potential novel prognostic marker for hepatocellular carcinoma
title_full_unstemmed	Nucleolar protein 6 promotes cell proliferation and acts as a potential novel prognostic marker for hepatocellular carcinoma
title_sort	nucleolar protein 6 promotes cell proliferation and acts as a potential novel prognostic marker for hepatocellular carcinoma
publisher	Wolters Kluwer
publishDate	2021
url	https://doaj.org/article/c147624976414902a3d5178e959c5a80
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Nucleolar protein 6 promotes cell proliferation and acts as a potential novel prognostic marker for hepatocellular carcinoma

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