Combined Evaluation of MAP1LC3B and SQSTM1 for Biological and Clinical Significance in Ductal Carcinoma of Breast Cancer

Breast cancer is the leading cause of cancer death in women worldwide. The microtubule-associated protein light chain 3B (MAP1LC3B) and adaptor sequestosome 1 (SQSTM1) are two major markers for autophagy. Increased protein levels of MAP1LC3B and SQSTM1 are considered to be causes of autophagy inhibi...

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Autores principales:	Pei-Feng Liu, Chih-Wen Shu, Hsiu-Chen Yang, Cheng-Hsin Lee, Huei-Han Liou, Luo-Ping Ger, Yen-Dun Tony Tzeng, Wen-Ching Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	invasive ductal carcinoma MAP1LC3B SQSTM1 autophagy inhibition chemoresistance prognosis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c14a1d12b6d4425d880982a056e78aa1
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spelling	oai:doaj.org-article:c14a1d12b6d4425d880982a056e78aa12021-11-25T16:48:26ZCombined Evaluation of MAP1LC3B and SQSTM1 for Biological and Clinical Significance in Ductal Carcinoma of Breast Cancer10.3390/biomedicines91115142227-9059https://doaj.org/article/c14a1d12b6d4425d880982a056e78aa12021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1514https://doaj.org/toc/2227-9059Breast cancer is the leading cause of cancer death in women worldwide. The microtubule-associated protein light chain 3B (MAP1LC3B) and adaptor sequestosome 1 (SQSTM1) are two major markers for autophagy. Increased protein levels of MAP1LC3B and SQSTM1 are considered to be causes of autophagy inhibition or activation in various types of cancers. However, the roles of MAP1LC3B and SQSTM1 in breast cancer are still not clear. Using a tissue microarray from 274 breast invasive ductal carcinoma (IDC) patients, we found that tumor tissues showed higher protein levels of MAP1LC3B and cytoplasmic SQSTM1 in comparison to those in adjacent normal tissues. Moreover, high levels of MAP1LC3B were associated with better survival, including disease-specific survival and disease-free survival (DFS) in IDC patients. Furthermore, high co-expression of MAP1LC3B and SQSTM1 was significantly associated with better DFS in IDC patients. Astonishingly, the autophagy inhibitor accumulated the protein levels of MAP1LC3B/SQSTM1 and enhanced the cytotoxic effects of cisplatin and paclitaxel in MCF7 and BT474 breast cancer cell lines, implying that autophagy inhibition might result in poor prognosis and chemosensitivity in IDC. Taken together, high co-expression of MAP1LC3B and SQSTM1 might serve as a potential diagnostic and prognostic biomarker for IDC patients.Pei-Feng LiuChih-Wen ShuHsiu-Chen YangCheng-Hsin LeeHuei-Han LiouLuo-Ping GerYen-Dun Tony TzengWen-Ching WangMDPI AGarticleinvasive ductal carcinomaMAP1LC3BSQSTM1autophagy inhibitionchemoresistanceprognosisBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1514, p 1514 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	invasive ductal carcinoma MAP1LC3B SQSTM1 autophagy inhibition chemoresistance prognosis Biology (General) QH301-705.5
spellingShingle	invasive ductal carcinoma MAP1LC3B SQSTM1 autophagy inhibition chemoresistance prognosis Biology (General) QH301-705.5 Pei-Feng Liu Chih-Wen Shu Hsiu-Chen Yang Cheng-Hsin Lee Huei-Han Liou Luo-Ping Ger Yen-Dun Tony Tzeng Wen-Ching Wang Combined Evaluation of MAP1LC3B and SQSTM1 for Biological and Clinical Significance in Ductal Carcinoma of Breast Cancer
description	Breast cancer is the leading cause of cancer death in women worldwide. The microtubule-associated protein light chain 3B (MAP1LC3B) and adaptor sequestosome 1 (SQSTM1) are two major markers for autophagy. Increased protein levels of MAP1LC3B and SQSTM1 are considered to be causes of autophagy inhibition or activation in various types of cancers. However, the roles of MAP1LC3B and SQSTM1 in breast cancer are still not clear. Using a tissue microarray from 274 breast invasive ductal carcinoma (IDC) patients, we found that tumor tissues showed higher protein levels of MAP1LC3B and cytoplasmic SQSTM1 in comparison to those in adjacent normal tissues. Moreover, high levels of MAP1LC3B were associated with better survival, including disease-specific survival and disease-free survival (DFS) in IDC patients. Furthermore, high co-expression of MAP1LC3B and SQSTM1 was significantly associated with better DFS in IDC patients. Astonishingly, the autophagy inhibitor accumulated the protein levels of MAP1LC3B/SQSTM1 and enhanced the cytotoxic effects of cisplatin and paclitaxel in MCF7 and BT474 breast cancer cell lines, implying that autophagy inhibition might result in poor prognosis and chemosensitivity in IDC. Taken together, high co-expression of MAP1LC3B and SQSTM1 might serve as a potential diagnostic and prognostic biomarker for IDC patients.
format	article
author	Pei-Feng Liu Chih-Wen Shu Hsiu-Chen Yang Cheng-Hsin Lee Huei-Han Liou Luo-Ping Ger Yen-Dun Tony Tzeng Wen-Ching Wang
author_facet	Pei-Feng Liu Chih-Wen Shu Hsiu-Chen Yang Cheng-Hsin Lee Huei-Han Liou Luo-Ping Ger Yen-Dun Tony Tzeng Wen-Ching Wang
author_sort	Pei-Feng Liu
title	Combined Evaluation of MAP1LC3B and SQSTM1 for Biological and Clinical Significance in Ductal Carcinoma of Breast Cancer
title_short	Combined Evaluation of MAP1LC3B and SQSTM1 for Biological and Clinical Significance in Ductal Carcinoma of Breast Cancer
title_full	Combined Evaluation of MAP1LC3B and SQSTM1 for Biological and Clinical Significance in Ductal Carcinoma of Breast Cancer
title_fullStr	Combined Evaluation of MAP1LC3B and SQSTM1 for Biological and Clinical Significance in Ductal Carcinoma of Breast Cancer
title_full_unstemmed	Combined Evaluation of MAP1LC3B and SQSTM1 for Biological and Clinical Significance in Ductal Carcinoma of Breast Cancer
title_sort	combined evaluation of map1lc3b and sqstm1 for biological and clinical significance in ductal carcinoma of breast cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c14a1d12b6d4425d880982a056e78aa1
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Combined Evaluation of MAP1LC3B and SQSTM1 for Biological and Clinical Significance in Ductal Carcinoma of Breast Cancer

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