Effects of long-term norepinephrine treatment on normal immortalized ovarian and fallopian tube cells

Abstract Sustained adrenergic stimulation by norepinephrine (NE) contributes to ovarian carcinoma metastasis and impairment of chemotherapy response. Although the effect of sustained NE stimulation in cancer progression is well established, less is known about its role in cancer initiation. To deter...

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Autores principales:	Sweta Dash, Sean Yoder, Tania Mesa, Andrew Smith, Ling Cen, Steven Eschrich, Guillermo N. Armaiz-Pena, Alvaro N. A. Monteiro
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c1562a4c5ba547debe29c88887771d9e
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spelling	oai:doaj.org-article:c1562a4c5ba547debe29c88887771d9e2021-12-02T15:33:12ZEffects of long-term norepinephrine treatment on normal immortalized ovarian and fallopian tube cells10.1038/s41598-021-93506-z2045-2322https://doaj.org/article/c1562a4c5ba547debe29c88887771d9e2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93506-zhttps://doaj.org/toc/2045-2322Abstract Sustained adrenergic stimulation by norepinephrine (NE) contributes to ovarian carcinoma metastasis and impairment of chemotherapy response. Although the effect of sustained NE stimulation in cancer progression is well established, less is known about its role in cancer initiation. To determine the extent to which stress hormones influence ovarian cancer initiation, we conducted a long-term (> 3 months; > 40 population doublings) experiment in which normal immortalized fallopian tube secretory (iFTSEC283) and ovarian surface epithelial (iOSE11) cell lines and their isogenic pairs containing a p53 mutation (iFTSEC283p53R175H; iOSE11p53R175H), were continuously exposed to NE (100 nM, 1 μM, 10 μM). Fallopian tube cells displayed a p53-independent increase in proliferation and colony-forming ability in response to NE, while ovarian surface epithelial cells displayed a p53-independent decrease in both assays. Fallopian tube cells with mutant p53 showed a mild loss of chromosomes and TP53 status was also a defining factor in transcriptional response of fallopian tube cells to long-term NE treatment.Sweta DashSean YoderTania MesaAndrew SmithLing CenSteven EschrichGuillermo N. Armaiz-PenaAlvaro N. A. MonteiroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sweta Dash Sean Yoder Tania Mesa Andrew Smith Ling Cen Steven Eschrich Guillermo N. Armaiz-Pena Alvaro N. A. Monteiro Effects of long-term norepinephrine treatment on normal immortalized ovarian and fallopian tube cells
description	Abstract Sustained adrenergic stimulation by norepinephrine (NE) contributes to ovarian carcinoma metastasis and impairment of chemotherapy response. Although the effect of sustained NE stimulation in cancer progression is well established, less is known about its role in cancer initiation. To determine the extent to which stress hormones influence ovarian cancer initiation, we conducted a long-term (> 3 months; > 40 population doublings) experiment in which normal immortalized fallopian tube secretory (iFTSEC283) and ovarian surface epithelial (iOSE11) cell lines and their isogenic pairs containing a p53 mutation (iFTSEC283p53R175H; iOSE11p53R175H), were continuously exposed to NE (100 nM, 1 μM, 10 μM). Fallopian tube cells displayed a p53-independent increase in proliferation and colony-forming ability in response to NE, while ovarian surface epithelial cells displayed a p53-independent decrease in both assays. Fallopian tube cells with mutant p53 showed a mild loss of chromosomes and TP53 status was also a defining factor in transcriptional response of fallopian tube cells to long-term NE treatment.
format	article
author	Sweta Dash Sean Yoder Tania Mesa Andrew Smith Ling Cen Steven Eschrich Guillermo N. Armaiz-Pena Alvaro N. A. Monteiro
author_facet	Sweta Dash Sean Yoder Tania Mesa Andrew Smith Ling Cen Steven Eschrich Guillermo N. Armaiz-Pena Alvaro N. A. Monteiro
author_sort	Sweta Dash
title	Effects of long-term norepinephrine treatment on normal immortalized ovarian and fallopian tube cells
title_short	Effects of long-term norepinephrine treatment on normal immortalized ovarian and fallopian tube cells
title_full	Effects of long-term norepinephrine treatment on normal immortalized ovarian and fallopian tube cells
title_fullStr	Effects of long-term norepinephrine treatment on normal immortalized ovarian and fallopian tube cells
title_full_unstemmed	Effects of long-term norepinephrine treatment on normal immortalized ovarian and fallopian tube cells
title_sort	effects of long-term norepinephrine treatment on normal immortalized ovarian and fallopian tube cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c1562a4c5ba547debe29c88887771d9e
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Effects of long-term norepinephrine treatment on normal immortalized ovarian and fallopian tube cells

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