Altered levels of circulating nuclear and mitochondrial DNA in patients with Papillary Thyroid Cancer

Abstract Papillary thyroid cancer is the most common thyroid cancer type. However, diagnostics based on fine needle biopsy cannot make a definitive diagnosis in 25% of thyroid nodules. Additionally, approximately 70% to 80% of thyroid lobectomies performed just for diagnostic purposes are benign. De...

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Autores principales:	Ewelina Perdas, Robert Stawski, Krzysztof Kaczka, Dariusz Nowak, Maria Zubrzycka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c15b98ada59c43a18b99f61418be104a
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spelling	oai:doaj.org-article:c15b98ada59c43a18b99f61418be104a2021-12-02T15:08:46ZAltered levels of circulating nuclear and mitochondrial DNA in patients with Papillary Thyroid Cancer10.1038/s41598-019-51000-72045-2322https://doaj.org/article/c15b98ada59c43a18b99f61418be104a2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-51000-7https://doaj.org/toc/2045-2322Abstract Papillary thyroid cancer is the most common thyroid cancer type. However, diagnostics based on fine needle biopsy cannot make a definitive diagnosis in 25% of thyroid nodules. Additionally, approximately 70% to 80% of thyroid lobectomies performed just for diagnostic purposes are benign. Despite this, biopsy still remains the main method of evaluation of thyroid nodules. Cell-free DNA (cf-DNA) measurement could give a new diagnostic opportunities which may reduce the number of unnecessary thyroid procedures. In this study, using a qPCR, we have examined the nuclear cf-DNA and mitochondrial cf-DNA in the plasma of 32 patients. We have found that the level of nuclear cf-DNA is almost 2-fold increased (median 3 089 vs. 1 872, p = 0.022), whereas mitochondrial cf-DNA content was significantly decreased in respect to healthy controls (median 44 992 vs. 92 220, p = 0.010). The ROC curve analysis showed high specificity for nuclear cf-DNA and mitochondrial cf-DNA, which may serve as a useful tool to decrease the number of unneeded surgeries. Our study reports the first epidemiological evidence for lower mitochondrial cf-DNA content in the patient group, what suggests that apart from nuclear cf-DNA also mitochondrial cf-DNA is affected by disease development.Ewelina PerdasRobert StawskiKrzysztof KaczkaDariusz NowakMaria ZubrzyckaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-7 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ewelina Perdas Robert Stawski Krzysztof Kaczka Dariusz Nowak Maria Zubrzycka Altered levels of circulating nuclear and mitochondrial DNA in patients with Papillary Thyroid Cancer
description	Abstract Papillary thyroid cancer is the most common thyroid cancer type. However, diagnostics based on fine needle biopsy cannot make a definitive diagnosis in 25% of thyroid nodules. Additionally, approximately 70% to 80% of thyroid lobectomies performed just for diagnostic purposes are benign. Despite this, biopsy still remains the main method of evaluation of thyroid nodules. Cell-free DNA (cf-DNA) measurement could give a new diagnostic opportunities which may reduce the number of unnecessary thyroid procedures. In this study, using a qPCR, we have examined the nuclear cf-DNA and mitochondrial cf-DNA in the plasma of 32 patients. We have found that the level of nuclear cf-DNA is almost 2-fold increased (median 3 089 vs. 1 872, p = 0.022), whereas mitochondrial cf-DNA content was significantly decreased in respect to healthy controls (median 44 992 vs. 92 220, p = 0.010). The ROC curve analysis showed high specificity for nuclear cf-DNA and mitochondrial cf-DNA, which may serve as a useful tool to decrease the number of unneeded surgeries. Our study reports the first epidemiological evidence for lower mitochondrial cf-DNA content in the patient group, what suggests that apart from nuclear cf-DNA also mitochondrial cf-DNA is affected by disease development.
format	article
author	Ewelina Perdas Robert Stawski Krzysztof Kaczka Dariusz Nowak Maria Zubrzycka
author_facet	Ewelina Perdas Robert Stawski Krzysztof Kaczka Dariusz Nowak Maria Zubrzycka
author_sort	Ewelina Perdas
title	Altered levels of circulating nuclear and mitochondrial DNA in patients with Papillary Thyroid Cancer
title_short	Altered levels of circulating nuclear and mitochondrial DNA in patients with Papillary Thyroid Cancer
title_full	Altered levels of circulating nuclear and mitochondrial DNA in patients with Papillary Thyroid Cancer
title_fullStr	Altered levels of circulating nuclear and mitochondrial DNA in patients with Papillary Thyroid Cancer
title_full_unstemmed	Altered levels of circulating nuclear and mitochondrial DNA in patients with Papillary Thyroid Cancer
title_sort	altered levels of circulating nuclear and mitochondrial dna in patients with papillary thyroid cancer
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/c15b98ada59c43a18b99f61418be104a
work_keys_str_mv	AT ewelinaperdas alteredlevelsofcirculatingnuclearandmitochondrialdnainpatientswithpapillarythyroidcancer AT robertstawski alteredlevelsofcirculatingnuclearandmitochondrialdnainpatientswithpapillarythyroidcancer AT krzysztofkaczka alteredlevelsofcirculatingnuclearandmitochondrialdnainpatientswithpapillarythyroidcancer AT dariusznowak alteredlevelsofcirculatingnuclearandmitochondrialdnainpatientswithpapillarythyroidcancer AT mariazubrzycka alteredlevelsofcirculatingnuclearandmitochondrialdnainpatientswithpapillarythyroidcancer
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Altered levels of circulating nuclear and mitochondrial DNA in patients with Papillary Thyroid Cancer

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