Mastrevirus Rep and RepA Proteins Suppress de novo Transcriptional Gene Silencing

Transcriptional gene silencing (TGS) in plants is a defense mechanism against DNA virus infection. The genomes of viruses in the <i>Geminiviridae</i> family encode several TGS suppressors. In this study, we induced de novo TGS against the transgenic <i>GFP</i> gene encoding g...

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Auteurs principaux:	Kikyo Watanabe, Masashi Ugaki
Format:	article
Langue:	EN
Publié:	MDPI AG 2021
Sujets:	<i>Geminivirus</i> TYDV transcriptional gene silencing silencing suppressor Rep RepA Biology (General) QH301-705.5 Chemistry QD1-999
Accès en ligne:	https://doaj.org/article/c15d9f3fd7a64b13a29e520480fb5d4b
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spelling	oai:doaj.org-article:c15d9f3fd7a64b13a29e520480fb5d4b2021-11-11T16:55:31ZMastrevirus Rep and RepA Proteins Suppress de novo Transcriptional Gene Silencing10.3390/ijms2221114621422-00671661-6596https://doaj.org/article/c15d9f3fd7a64b13a29e520480fb5d4b2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11462https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Transcriptional gene silencing (TGS) in plants is a defense mechanism against DNA virus infection. The genomes of viruses in the <i>Geminiviridae</i> family encode several TGS suppressors. In this study, we induced de novo TGS against the transgenic <i>GFP</i> gene encoding green fluorescent protein by expressing a hairpin-shaped self-complementary RNA corresponding to the enhancer region of the 35S promoter (hpE35S). In addition, we examined the TGS suppression activity of proteins encoded in the genome of <i>Tobacco yellow dwarf virus</i> (TYDV, genus <i>Mastrevirus</i>). The results show that the replication-associated protein (Rep) and RepA encoded by TYDV have TGS suppressor activity and lead to decreased accumulation of 24-nt siRNAs. These results suggest that Rep and RepA can block the steps before the loading of siRNAs into Argonaute (AGO) proteins. This is the first report of TGS suppressors in the genus <i>Mastrevirus</i>.Kikyo WatanabeMasashi UgakiMDPI AGarticle<i>Geminivirus</i>TYDVtranscriptional gene silencingsilencing suppressorRepRepABiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11462, p 11462 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Geminivirus</i> TYDV transcriptional gene silencing silencing suppressor Rep RepA Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Geminivirus</i> TYDV transcriptional gene silencing silencing suppressor Rep RepA Biology (General) QH301-705.5 Chemistry QD1-999 Kikyo Watanabe Masashi Ugaki Mastrevirus Rep and RepA Proteins Suppress de novo Transcriptional Gene Silencing
description	Transcriptional gene silencing (TGS) in plants is a defense mechanism against DNA virus infection. The genomes of viruses in the <i>Geminiviridae</i> family encode several TGS suppressors. In this study, we induced de novo TGS against the transgenic <i>GFP</i> gene encoding green fluorescent protein by expressing a hairpin-shaped self-complementary RNA corresponding to the enhancer region of the 35S promoter (hpE35S). In addition, we examined the TGS suppression activity of proteins encoded in the genome of <i>Tobacco yellow dwarf virus</i> (TYDV, genus <i>Mastrevirus</i>). The results show that the replication-associated protein (Rep) and RepA encoded by TYDV have TGS suppressor activity and lead to decreased accumulation of 24-nt siRNAs. These results suggest that Rep and RepA can block the steps before the loading of siRNAs into Argonaute (AGO) proteins. This is the first report of TGS suppressors in the genus <i>Mastrevirus</i>.
format	article
author	Kikyo Watanabe Masashi Ugaki
author_facet	Kikyo Watanabe Masashi Ugaki
author_sort	Kikyo Watanabe
title	Mastrevirus Rep and RepA Proteins Suppress de novo Transcriptional Gene Silencing
title_short	Mastrevirus Rep and RepA Proteins Suppress de novo Transcriptional Gene Silencing
title_full	Mastrevirus Rep and RepA Proteins Suppress de novo Transcriptional Gene Silencing
title_fullStr	Mastrevirus Rep and RepA Proteins Suppress de novo Transcriptional Gene Silencing
title_full_unstemmed	Mastrevirus Rep and RepA Proteins Suppress de novo Transcriptional Gene Silencing
title_sort	mastrevirus rep and repa proteins suppress de novo transcriptional gene silencing
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c15d9f3fd7a64b13a29e520480fb5d4b
work_keys_str_mv	AT kikyowatanabe mastrevirusrepandrepaproteinssuppressdenovotranscriptionalgenesilencing AT masashiugaki mastrevirusrepandrepaproteinssuppressdenovotranscriptionalgenesilencing
_version_	1718432201177038848

Mastrevirus Rep and RepA Proteins Suppress de novo Transcriptional Gene Silencing

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