Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice

Abstract Evidence has demonstrated that microRNA-342-5p (miR-342-5p) is implicated in atherosclerosis (AS), but little is known regarding its intrinsic regulatory mechanisms. Here, we aimed to explore the effect of miR-342-5p targeting Wnt3a on formation of vulnerable plaques and angiogenesis of AS....

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Autores principales:	Haixia Sun, Jinhua Feng, Yan Ma, Ding Cai, Yulu Luo, Qinggong Wang, Fang Li, Mingyue Zhang, Quanzhong Hu
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Atherosclerosis MicroRNA-342-5p Wnt3a Vulnerable plaque Vulnerability index Microvessel density Materials of engineering and construction. Mechanics of materials TA401-492
Acceso en línea:	https://doaj.org/article/c15eb2500ef642e9aeefb8639cf10345
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spelling	oai:doaj.org-article:c15eb2500ef642e9aeefb8639cf103452021-11-28T12:37:26ZDown-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice10.1186/s11671-021-03608-w1556-276Xhttps://doaj.org/article/c15eb2500ef642e9aeefb8639cf103452021-11-01T00:00:00Zhttps://doi.org/10.1186/s11671-021-03608-whttps://doaj.org/toc/1556-276XAbstract Evidence has demonstrated that microRNA-342-5p (miR-342-5p) is implicated in atherosclerosis (AS), but little is known regarding its intrinsic regulatory mechanisms. Here, we aimed to explore the effect of miR-342-5p targeting Wnt3a on formation of vulnerable plaques and angiogenesis of AS. ApoE−/− mice were fed with high-fat feed for 16 w to replicate the AS vulnerable plaque model. miR-342-5p and Wnt3a expression in aortic tissues of AS were detected. The target relationship between miR-342-5p and Wnt3a was verified. Moreover, ApoE−/− mice were injected with miR-342-5p antagomir and overexpression-Wnt3a vector to test their functions in serum lipid levels, inflammatory and oxidative stress-related cytokines, aortic plaque stability and angiogenesis in plaque of AS mice. miR-342-5p expression was enhanced and Wnt3a expression was degraded in aortic tissues of AS mice and miR-342-5p directly targeted Wnt3a. Up-regulating Wnt3a or down-regulating miR-342-5p reduced blood lipid content, inflammatory and oxidative stress levels, the vulnerability of aortic tissue plaque and inhibited angiogenesis in aortic plaque of AS mice. Functional studies show that depleting miR-342-5p can stabilize aortic tissue plaque and reduce angiogenesis in plaque in AS mice via restoring Wnt3a.Haixia SunJinhua FengYan MaDing CaiYulu LuoQinggong WangFang LiMingyue ZhangQuanzhong HuSpringerOpenarticleAtherosclerosisMicroRNA-342-5pWnt3aVulnerable plaqueVulnerability indexMicrovessel densityMaterials of engineering and construction. Mechanics of materialsTA401-492ENNanoscale Research Letters, Vol 16, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Atherosclerosis MicroRNA-342-5p Wnt3a Vulnerable plaque Vulnerability index Microvessel density Materials of engineering and construction. Mechanics of materials TA401-492
spellingShingle	Atherosclerosis MicroRNA-342-5p Wnt3a Vulnerable plaque Vulnerability index Microvessel density Materials of engineering and construction. Mechanics of materials TA401-492 Haixia Sun Jinhua Feng Yan Ma Ding Cai Yulu Luo Qinggong Wang Fang Li Mingyue Zhang Quanzhong Hu Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice
description	Abstract Evidence has demonstrated that microRNA-342-5p (miR-342-5p) is implicated in atherosclerosis (AS), but little is known regarding its intrinsic regulatory mechanisms. Here, we aimed to explore the effect of miR-342-5p targeting Wnt3a on formation of vulnerable plaques and angiogenesis of AS. ApoE−/− mice were fed with high-fat feed for 16 w to replicate the AS vulnerable plaque model. miR-342-5p and Wnt3a expression in aortic tissues of AS were detected. The target relationship between miR-342-5p and Wnt3a was verified. Moreover, ApoE−/− mice were injected with miR-342-5p antagomir and overexpression-Wnt3a vector to test their functions in serum lipid levels, inflammatory and oxidative stress-related cytokines, aortic plaque stability and angiogenesis in plaque of AS mice. miR-342-5p expression was enhanced and Wnt3a expression was degraded in aortic tissues of AS mice and miR-342-5p directly targeted Wnt3a. Up-regulating Wnt3a or down-regulating miR-342-5p reduced blood lipid content, inflammatory and oxidative stress levels, the vulnerability of aortic tissue plaque and inhibited angiogenesis in aortic plaque of AS mice. Functional studies show that depleting miR-342-5p can stabilize aortic tissue plaque and reduce angiogenesis in plaque in AS mice via restoring Wnt3a.
format	article
author	Haixia Sun Jinhua Feng Yan Ma Ding Cai Yulu Luo Qinggong Wang Fang Li Mingyue Zhang Quanzhong Hu
author_facet	Haixia Sun Jinhua Feng Yan Ma Ding Cai Yulu Luo Qinggong Wang Fang Li Mingyue Zhang Quanzhong Hu
author_sort	Haixia Sun
title	Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice
title_short	Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice
title_full	Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice
title_fullStr	Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice
title_full_unstemmed	Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice
title_sort	down-regulation of microrna-342-5p or up-regulation of wnt3a inhibits angiogenesis and maintains atherosclerotic plaque stability in atherosclerosis mice
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/c15eb2500ef642e9aeefb8639cf10345
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Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice

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