SOME WAYS OF PRODUCTION PROCESS OF LIVE PLAGUE VACCINE AND TECHNIQUES OF EVALUATION THE IMMUNOGENICITY (REVIEW)

The modern findings of scientific research in a live plague vaccine range such as production antibiotic-resistant subcultures of the vaccine strains, design of the low-development of reaction producers vaccine strains, production of oral (tablet) preparation are represented in the review. Some techn...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zh. A. Konovalova, A. G. Atlas, V. I. Dubrovina
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2013
Materias:	plague agent live plague vaccine immunogenicity Science Q
Acceso en línea:	https://doaj.org/article/c16165a5f69b408399656dcc8656157f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c16165a5f69b408399656dcc8656157f
record_format	dspace
spelling	oai:doaj.org-article:c16165a5f69b408399656dcc8656157f2021-11-23T06:14:26ZSOME WAYS OF PRODUCTION PROCESS OF LIVE PLAGUE VACCINE AND TECHNIQUES OF EVALUATION THE IMMUNOGENICITY (REVIEW)2541-94202587-9596https://doaj.org/article/c16165a5f69b408399656dcc8656157f2013-04-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/1473https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596The modern findings of scientific research in a live plague vaccine range such as production antibiotic-resistant subcultures of the vaccine strains, design of the low-development of reaction producers vaccine strains, production of oral (tablet) preparation are represented in the review. Some techniques of production improvement live plague vaccine are formulated: stabilization of a number alive microbial cells in a inoculation dose of a license vaccine by decreasing of cultivation temperature mode from 27 °C till 21°С, concentration of microbial cells in ampoule from (5-10) × 1010 till (1-4) × 1010 per 1 ml, reduction freezing-drying step from 28 till 18 h, decreasing of amount dose in ampoule from 100-125 till 20-50 and furthermore acceleration of production process of microbial cells concentrate (fermentation in the bioreactor, tangential microfiltration) and formulation combined (meat-soya nutritious base) medium and conditions of their preparation (time reduction enzymatic hydrolysis from 14 till 3 days). The tendenceis of refinement of a technological process of equipment link were researched, such as using bioreactors instead of apparatusfor a cultivation microbesShesterenko, disposable bags Flexboy, which are allowed to eliminate the application of glass bottles and operation of a bottle assembling, also using of robotizing sublimation installations for drying of a liquid form of live plague vaccine, which are allowed to exclude utilization of low-temperature refrigerators. Beside of regulating estimation method of immunogenicity (calculation of effective immunizing dose) and quantitative account of a phenomenon of survival white mice the cyto-enzymochemical and morphometric rates are suggested to estimate the quality of ultimate product live plague dry vaccine, changes of those are charactering immunologic activity and state of mobilizing and bactericidal abilities of a organism phagocyte system cells.Zh. A. KonovalovaA. G. AtlasV. I. DubrovinaScientific Сentre for Family Health and Human Reproduction Problemsarticleplague agentlive plague vaccineimmunogenicityScienceQRUActa Biomedica Scientifica, Vol 0, Iss 2(2), Pp 192-196 (2013)
institution	DOAJ
collection	DOAJ
language	RU
topic	plague agent live plague vaccine immunogenicity Science Q
spellingShingle	plague agent live plague vaccine immunogenicity Science Q Zh. A. Konovalova A. G. Atlas V. I. Dubrovina SOME WAYS OF PRODUCTION PROCESS OF LIVE PLAGUE VACCINE AND TECHNIQUES OF EVALUATION THE IMMUNOGENICITY (REVIEW)
description	The modern findings of scientific research in a live plague vaccine range such as production antibiotic-resistant subcultures of the vaccine strains, design of the low-development of reaction producers vaccine strains, production of oral (tablet) preparation are represented in the review. Some techniques of production improvement live plague vaccine are formulated: stabilization of a number alive microbial cells in a inoculation dose of a license vaccine by decreasing of cultivation temperature mode from 27 °C till 21°С, concentration of microbial cells in ampoule from (5-10) × 1010 till (1-4) × 1010 per 1 ml, reduction freezing-drying step from 28 till 18 h, decreasing of amount dose in ampoule from 100-125 till 20-50 and furthermore acceleration of production process of microbial cells concentrate (fermentation in the bioreactor, tangential microfiltration) and formulation combined (meat-soya nutritious base) medium and conditions of their preparation (time reduction enzymatic hydrolysis from 14 till 3 days). The tendenceis of refinement of a technological process of equipment link were researched, such as using bioreactors instead of apparatusfor a cultivation microbesShesterenko, disposable bags Flexboy, which are allowed to eliminate the application of glass bottles and operation of a bottle assembling, also using of robotizing sublimation installations for drying of a liquid form of live plague vaccine, which are allowed to exclude utilization of low-temperature refrigerators. Beside of regulating estimation method of immunogenicity (calculation of effective immunizing dose) and quantitative account of a phenomenon of survival white mice the cyto-enzymochemical and morphometric rates are suggested to estimate the quality of ultimate product live plague dry vaccine, changes of those are charactering immunologic activity and state of mobilizing and bactericidal abilities of a organism phagocyte system cells.
format	article
author	Zh. A. Konovalova A. G. Atlas V. I. Dubrovina
author_facet	Zh. A. Konovalova A. G. Atlas V. I. Dubrovina
author_sort	Zh. A. Konovalova
title	SOME WAYS OF PRODUCTION PROCESS OF LIVE PLAGUE VACCINE AND TECHNIQUES OF EVALUATION THE IMMUNOGENICITY (REVIEW)
title_short	SOME WAYS OF PRODUCTION PROCESS OF LIVE PLAGUE VACCINE AND TECHNIQUES OF EVALUATION THE IMMUNOGENICITY (REVIEW)
title_full	SOME WAYS OF PRODUCTION PROCESS OF LIVE PLAGUE VACCINE AND TECHNIQUES OF EVALUATION THE IMMUNOGENICITY (REVIEW)
title_fullStr	SOME WAYS OF PRODUCTION PROCESS OF LIVE PLAGUE VACCINE AND TECHNIQUES OF EVALUATION THE IMMUNOGENICITY (REVIEW)
title_full_unstemmed	SOME WAYS OF PRODUCTION PROCESS OF LIVE PLAGUE VACCINE AND TECHNIQUES OF EVALUATION THE IMMUNOGENICITY (REVIEW)
title_sort	some ways of production process of live plague vaccine and techniques of evaluation the immunogenicity (review)
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2013
url	https://doaj.org/article/c16165a5f69b408399656dcc8656157f
work_keys_str_mv	AT zhakonovalova somewaysofproductionprocessofliveplaguevaccineandtechniquesofevaluationtheimmunogenicityreview AT agatlas somewaysofproductionprocessofliveplaguevaccineandtechniquesofevaluationtheimmunogenicityreview AT vidubrovina somewaysofproductionprocessofliveplaguevaccineandtechniquesofevaluationtheimmunogenicityreview
_version_	1718417220363616256

SOME WAYS OF PRODUCTION PROCESS OF LIVE PLAGUE VACCINE AND TECHNIQUES OF EVALUATION THE IMMUNOGENICITY (REVIEW)

Ejemplares similares