Synthesis, Characterization and Anti-Cancer Therapeutic Potential of Withanolide-A with 20nm sAuNPs Conjugates Against SKBR3 Breast Cancer Cell Line

Qudsia Tabassam,1 Tahir Mehmood,1,2 Abdul Rauf Raza,1 Azmat Ullah,3 Farhan Saeed,4 Faqir Muhammad Anjum5 1Department of Chemistry, University of Sargodha, Sargodha, Pakistan; 2Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences-UVAS, Lahore, Pakistan; 3Departmen...

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Autores principales:	Tabassam Q, Mehmood T, Raza AR, Ullah A, Saeed F, Anjum FM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	withania somnifera withanolide a gold nano-particle breast cancer anti-cancer and incucyte Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c162e0b094f34e3d8323f5016480272b
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spelling	oai:doaj.org-article:c162e0b094f34e3d8323f5016480272b2021-12-02T09:59:24ZSynthesis, Characterization and Anti-Cancer Therapeutic Potential of Withanolide-A with 20nm sAuNPs Conjugates Against SKBR3 Breast Cancer Cell Line1178-2013https://doaj.org/article/c162e0b094f34e3d8323f5016480272b2020-09-01T00:00:00Zhttps://www.dovepress.com/synthesis-characterization-and-anti-cancer-therapeutic-potential-of-wi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Qudsia Tabassam,1 Tahir Mehmood,1,2 Abdul Rauf Raza,1 Azmat Ullah,3 Farhan Saeed,4 Faqir Muhammad Anjum5 1Department of Chemistry, University of Sargodha, Sargodha, Pakistan; 2Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences-UVAS, Lahore, Pakistan; 3Department of Food Science and Human Nutrition, University of Veterinary and Animal Sciences-UVAS, Lahore, Pakistan; 4Institute of Home & Food Sciences, Government College University Faisalabad, Faisalabad, Pakistan; 5Vice Chancellor Secretariat, University of the Gambia, Banjul, The GambiaCorrespondence: Tahir Mehmood; Faqir Muhammad Anjum Email tahir.mehmoodbiochem@uvas.edu.pk; dranjum@utg.edu.gmBackground: Nanotechnology is gaining emerging interest in advanced drug discovery therapeutics due to their tremendous properties including enhanced delivery of therapeutic payload, extensive surface to volume ratio, high permeability, retention behaviors, etc. The gold nanoparticles (AuNPs) are favored due to their advanced features, such as biogenic, tunable physiochemical response, ease in synthesis, and wide range of biomedical applications. The phytochemicals have been focused to design Au nano-carrier-based conjugation for active-targeting drug delivery due to their nano conjugation ability.Aim: The present study describes the facile synthesis of 20nm spherical AuNPs and their conjugation with reported anti-cancer phytocompound Withanolide-A (1).Methods: The 20nm sAuNPs were synthesized chemically and characterized their phytochemical gold nanoconjugates through UV-visible spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM) imaging techniques. The anti-cancer therapeutic potentials were tested with both nanoconjugates and pure WithanolideA (1) by using SKBR3 breast cancer cells line.Results: The synthesized sAuNPs showed significant conjugation with Withanolide-A and showed stability. Furthermore, these Au nanoconjugates with Withanolide-A (1) significantly induce blockage of SKBR3 cell growth at half maximal active concentration that compared to pure Withanolide-A (1).Conclusion: Our findings provide a foundation to further progress how they can overcome cancer drug resistance by conjugating active drugs in combination with AuNPs through optimizing the effective drug concentration and removing the surface barrier.Keywords: Withania somnifera, Withanolide-A, gold nanoparticle; AuNP, breast cancer, anti-cancer and incucyteTabassam QMehmood TRaza ARUllah ASaeed FAnjum FMDove Medical Pressarticlewithania somniferawithanolide agold nano-particlebreast canceranti-cancer and incucyteMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 6649-6658 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	withania somnifera withanolide a gold nano-particle breast cancer anti-cancer and incucyte Medicine (General) R5-920
spellingShingle	withania somnifera withanolide a gold nano-particle breast cancer anti-cancer and incucyte Medicine (General) R5-920 Tabassam Q Mehmood T Raza AR Ullah A Saeed F Anjum FM Synthesis, Characterization and Anti-Cancer Therapeutic Potential of Withanolide-A with 20nm sAuNPs Conjugates Against SKBR3 Breast Cancer Cell Line
description	Qudsia Tabassam,1 Tahir Mehmood,1,2 Abdul Rauf Raza,1 Azmat Ullah,3 Farhan Saeed,4 Faqir Muhammad Anjum5 1Department of Chemistry, University of Sargodha, Sargodha, Pakistan; 2Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences-UVAS, Lahore, Pakistan; 3Department of Food Science and Human Nutrition, University of Veterinary and Animal Sciences-UVAS, Lahore, Pakistan; 4Institute of Home & Food Sciences, Government College University Faisalabad, Faisalabad, Pakistan; 5Vice Chancellor Secretariat, University of the Gambia, Banjul, The GambiaCorrespondence: Tahir Mehmood; Faqir Muhammad Anjum Email tahir.mehmoodbiochem@uvas.edu.pk; dranjum@utg.edu.gmBackground: Nanotechnology is gaining emerging interest in advanced drug discovery therapeutics due to their tremendous properties including enhanced delivery of therapeutic payload, extensive surface to volume ratio, high permeability, retention behaviors, etc. The gold nanoparticles (AuNPs) are favored due to their advanced features, such as biogenic, tunable physiochemical response, ease in synthesis, and wide range of biomedical applications. The phytochemicals have been focused to design Au nano-carrier-based conjugation for active-targeting drug delivery due to their nano conjugation ability.Aim: The present study describes the facile synthesis of 20nm spherical AuNPs and their conjugation with reported anti-cancer phytocompound Withanolide-A (1).Methods: The 20nm sAuNPs were synthesized chemically and characterized their phytochemical gold nanoconjugates through UV-visible spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM) imaging techniques. The anti-cancer therapeutic potentials were tested with both nanoconjugates and pure WithanolideA (1) by using SKBR3 breast cancer cells line.Results: The synthesized sAuNPs showed significant conjugation with Withanolide-A and showed stability. Furthermore, these Au nanoconjugates with Withanolide-A (1) significantly induce blockage of SKBR3 cell growth at half maximal active concentration that compared to pure Withanolide-A (1).Conclusion: Our findings provide a foundation to further progress how they can overcome cancer drug resistance by conjugating active drugs in combination with AuNPs through optimizing the effective drug concentration and removing the surface barrier.Keywords: Withania somnifera, Withanolide-A, gold nanoparticle; AuNP, breast cancer, anti-cancer and incucyte
format	article
author	Tabassam Q Mehmood T Raza AR Ullah A Saeed F Anjum FM
author_facet	Tabassam Q Mehmood T Raza AR Ullah A Saeed F Anjum FM
author_sort	Tabassam Q
title	Synthesis, Characterization and Anti-Cancer Therapeutic Potential of Withanolide-A with 20nm sAuNPs Conjugates Against SKBR3 Breast Cancer Cell Line
title_short	Synthesis, Characterization and Anti-Cancer Therapeutic Potential of Withanolide-A with 20nm sAuNPs Conjugates Against SKBR3 Breast Cancer Cell Line
title_full	Synthesis, Characterization and Anti-Cancer Therapeutic Potential of Withanolide-A with 20nm sAuNPs Conjugates Against SKBR3 Breast Cancer Cell Line
title_fullStr	Synthesis, Characterization and Anti-Cancer Therapeutic Potential of Withanolide-A with 20nm sAuNPs Conjugates Against SKBR3 Breast Cancer Cell Line
title_full_unstemmed	Synthesis, Characterization and Anti-Cancer Therapeutic Potential of Withanolide-A with 20nm sAuNPs Conjugates Against SKBR3 Breast Cancer Cell Line
title_sort	synthesis, characterization and anti-cancer therapeutic potential of withanolide-a with 20nm saunps conjugates against skbr3 breast cancer cell line
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/c162e0b094f34e3d8323f5016480272b
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Synthesis, Characterization and Anti-Cancer Therapeutic Potential of Withanolide-A with 20nm sAuNPs Conjugates Against SKBR3 Breast Cancer Cell Line

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