Genetic tools to study juvenile hormone action in Drosophila

Abstract The insect juvenile hormone receptor is a basic helix-loop-helix (bHLH), Per-Arnt-Sim (PAS) domain protein, a novel type of hormone receptor. In higher flies like Drosophila, the ancestral receptor germ cell-expressed (gce) gene has duplicated to yield the paralog Methoprene-tolerant (Met)....

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Autores principales:	A. A. Baumann, M. J. Texada, H. M. Chen, J. N. Etheredge, D. L. Miller, S. Picard, R. Warner, J. W. Truman, L. M. Riddiford
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c166d1006240409296d9fa7d6319a0f4
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spelling	oai:doaj.org-article:c166d1006240409296d9fa7d6319a0f42021-12-02T12:30:52ZGenetic tools to study juvenile hormone action in Drosophila10.1038/s41598-017-02264-42045-2322https://doaj.org/article/c166d1006240409296d9fa7d6319a0f42017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02264-4https://doaj.org/toc/2045-2322Abstract The insect juvenile hormone receptor is a basic helix-loop-helix (bHLH), Per-Arnt-Sim (PAS) domain protein, a novel type of hormone receptor. In higher flies like Drosophila, the ancestral receptor germ cell-expressed (gce) gene has duplicated to yield the paralog Methoprene-tolerant (Met). These paralogous receptors share redundant function during development but play unique roles in adults. Some aspects of JH function apparently require one receptor or the other. To provide a foundation for studying JH receptor function, we have recapitulated endogenous JH receptor expression with single cell resolution. Using Bacteria Artificial Chromosome (BAC) recombineering and a transgenic knock-in, we have generated a spatiotemporal expressional atlas of Met and gce throughout development. We demonstrate JH receptor expression in known JH target tissues, in which temporal expression corresponds with periods of hormone sensitivity. Larval expression largely supports the notion of functional redundancy. Furthermore, we provide the neuroanatomical distribution of JH receptors in both the larval and adult central nervous system, which will serve as a platform for future studies regarding JH action on insect behavior.A. A. BaumannM. J. TexadaH. M. ChenJ. N. EtheredgeD. L. MillerS. PicardR. WarnerJ. W. TrumanL. M. RiddifordNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. A. Baumann M. J. Texada H. M. Chen J. N. Etheredge D. L. Miller S. Picard R. Warner J. W. Truman L. M. Riddiford Genetic tools to study juvenile hormone action in Drosophila
description	Abstract The insect juvenile hormone receptor is a basic helix-loop-helix (bHLH), Per-Arnt-Sim (PAS) domain protein, a novel type of hormone receptor. In higher flies like Drosophila, the ancestral receptor germ cell-expressed (gce) gene has duplicated to yield the paralog Methoprene-tolerant (Met). These paralogous receptors share redundant function during development but play unique roles in adults. Some aspects of JH function apparently require one receptor or the other. To provide a foundation for studying JH receptor function, we have recapitulated endogenous JH receptor expression with single cell resolution. Using Bacteria Artificial Chromosome (BAC) recombineering and a transgenic knock-in, we have generated a spatiotemporal expressional atlas of Met and gce throughout development. We demonstrate JH receptor expression in known JH target tissues, in which temporal expression corresponds with periods of hormone sensitivity. Larval expression largely supports the notion of functional redundancy. Furthermore, we provide the neuroanatomical distribution of JH receptors in both the larval and adult central nervous system, which will serve as a platform for future studies regarding JH action on insect behavior.
format	article
author	A. A. Baumann M. J. Texada H. M. Chen J. N. Etheredge D. L. Miller S. Picard R. Warner J. W. Truman L. M. Riddiford
author_facet	A. A. Baumann M. J. Texada H. M. Chen J. N. Etheredge D. L. Miller S. Picard R. Warner J. W. Truman L. M. Riddiford
author_sort	A. A. Baumann
title	Genetic tools to study juvenile hormone action in Drosophila
title_short	Genetic tools to study juvenile hormone action in Drosophila
title_full	Genetic tools to study juvenile hormone action in Drosophila
title_fullStr	Genetic tools to study juvenile hormone action in Drosophila
title_full_unstemmed	Genetic tools to study juvenile hormone action in Drosophila
title_sort	genetic tools to study juvenile hormone action in drosophila
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c166d1006240409296d9fa7d6319a0f4
work_keys_str_mv	AT aabaumann genetictoolstostudyjuvenilehormoneactionindrosophila AT mjtexada genetictoolstostudyjuvenilehormoneactionindrosophila AT hmchen genetictoolstostudyjuvenilehormoneactionindrosophila AT jnetheredge genetictoolstostudyjuvenilehormoneactionindrosophila AT dlmiller genetictoolstostudyjuvenilehormoneactionindrosophila AT spicard genetictoolstostudyjuvenilehormoneactionindrosophila AT rwarner genetictoolstostudyjuvenilehormoneactionindrosophila AT jwtruman genetictoolstostudyjuvenilehormoneactionindrosophila AT lmriddiford genetictoolstostudyjuvenilehormoneactionindrosophila
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Genetic tools to study juvenile hormone action in Drosophila

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