Three-dimensional scoring of zebrafish behavior unveils biological phenomena hidden by two-dimensional analyses

Abstract The study of zebrafish behavior represents a cornerstone upon which basic researchers promise to advance knowledge in life sciences. Although zebrafish swim in a three-dimensional (3D) space, their behavior in the lab is almost exclusively scored in two dimensions, whereby zebrafish are rec...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Simone Macrì, Daniele Neri, Tommaso Ruberto, Violet Mwaffo, Sachit Butail, Maurizio Porfiri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c16816d1527b4c0dabcae725e220b2a7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c16816d1527b4c0dabcae725e220b2a7
record_format	dspace
spelling	oai:doaj.org-article:c16816d1527b4c0dabcae725e220b2a72021-12-02T12:32:38ZThree-dimensional scoring of zebrafish behavior unveils biological phenomena hidden by two-dimensional analyses10.1038/s41598-017-01990-z2045-2322https://doaj.org/article/c16816d1527b4c0dabcae725e220b2a72017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01990-zhttps://doaj.org/toc/2045-2322Abstract The study of zebrafish behavior represents a cornerstone upon which basic researchers promise to advance knowledge in life sciences. Although zebrafish swim in a three-dimensional (3D) space, their behavior in the lab is almost exclusively scored in two dimensions, whereby zebrafish are recorded using a single camera providing 2D videos. Whether this dimensional reduction preserves the reliability of data has not been addressed. Here we show that, compared to a 3D observation, 2D data are flawed by over-reporting and under-reporting of locomotory differences. Specifically, we first reconstructed 3D trajectories through the integration of synchronous information derived from two cameras, and then compared them with the original 2D views in classical experimental paradigms assessing shoaling tendency, fear, anxiety, and general locomotion. Our results suggest that traditional behavioral scoring of individual zebrafish performed in 2D may undermine data integrity, thereby requiring a general reconsideration of scoring zebrafish behavior to incorporate a 3D approach. We then demonstrate that, compared to 2D, a 3D approach requires a reduced number of subjects to achieve the same degree of validity. We anticipate these findings to largely benefit animal welfare by reducing the number of experimental subjects, without affecting statistical power.Simone MacrìDaniele NeriTommaso RubertoViolet MwaffoSachit ButailMaurizio PorfiriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simone Macrì Daniele Neri Tommaso Ruberto Violet Mwaffo Sachit Butail Maurizio Porfiri Three-dimensional scoring of zebrafish behavior unveils biological phenomena hidden by two-dimensional analyses
description	Abstract The study of zebrafish behavior represents a cornerstone upon which basic researchers promise to advance knowledge in life sciences. Although zebrafish swim in a three-dimensional (3D) space, their behavior in the lab is almost exclusively scored in two dimensions, whereby zebrafish are recorded using a single camera providing 2D videos. Whether this dimensional reduction preserves the reliability of data has not been addressed. Here we show that, compared to a 3D observation, 2D data are flawed by over-reporting and under-reporting of locomotory differences. Specifically, we first reconstructed 3D trajectories through the integration of synchronous information derived from two cameras, and then compared them with the original 2D views in classical experimental paradigms assessing shoaling tendency, fear, anxiety, and general locomotion. Our results suggest that traditional behavioral scoring of individual zebrafish performed in 2D may undermine data integrity, thereby requiring a general reconsideration of scoring zebrafish behavior to incorporate a 3D approach. We then demonstrate that, compared to 2D, a 3D approach requires a reduced number of subjects to achieve the same degree of validity. We anticipate these findings to largely benefit animal welfare by reducing the number of experimental subjects, without affecting statistical power.
format	article
author	Simone Macrì Daniele Neri Tommaso Ruberto Violet Mwaffo Sachit Butail Maurizio Porfiri
author_facet	Simone Macrì Daniele Neri Tommaso Ruberto Violet Mwaffo Sachit Butail Maurizio Porfiri
author_sort	Simone Macrì
title	Three-dimensional scoring of zebrafish behavior unveils biological phenomena hidden by two-dimensional analyses
title_short	Three-dimensional scoring of zebrafish behavior unveils biological phenomena hidden by two-dimensional analyses
title_full	Three-dimensional scoring of zebrafish behavior unveils biological phenomena hidden by two-dimensional analyses
title_fullStr	Three-dimensional scoring of zebrafish behavior unveils biological phenomena hidden by two-dimensional analyses
title_full_unstemmed	Three-dimensional scoring of zebrafish behavior unveils biological phenomena hidden by two-dimensional analyses
title_sort	three-dimensional scoring of zebrafish behavior unveils biological phenomena hidden by two-dimensional analyses
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c16816d1527b4c0dabcae725e220b2a7
work_keys_str_mv	AT simonemacri threedimensionalscoringofzebrafishbehaviorunveilsbiologicalphenomenahiddenbytwodimensionalanalyses AT danieleneri threedimensionalscoringofzebrafishbehaviorunveilsbiologicalphenomenahiddenbytwodimensionalanalyses AT tommasoruberto threedimensionalscoringofzebrafishbehaviorunveilsbiologicalphenomenahiddenbytwodimensionalanalyses AT violetmwaffo threedimensionalscoringofzebrafishbehaviorunveilsbiologicalphenomenahiddenbytwodimensionalanalyses AT sachitbutail threedimensionalscoringofzebrafishbehaviorunveilsbiologicalphenomenahiddenbytwodimensionalanalyses AT maurizioporfiri threedimensionalscoringofzebrafishbehaviorunveilsbiologicalphenomenahiddenbytwodimensionalanalyses
_version_	1718394003191234560

Three-dimensional scoring of zebrafish behavior unveils biological phenomena hidden by two-dimensional analyses

Ejemplares similares