Immunogenicity of Novel Live Vaccine Based on an Artificial rHN20 Strain against Emerging Fowl Adenovirus 4

In recent years, hepatitis-hydropericardium syndrome (HHS), caused by novel fowl adenovirus 4 (FAdV-4), has caused serious economic losses to the poultry industry. Vaccines are important for preventing and controlling HHS. Current FAdV-4 vaccine research and development are mainly focuses on inactiv...

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Autores principales:	Yu Zhang, Qing Pan, Rongrong Guo, Aijing Liu, Zhuangzhuang Xu, Yulong Gao, Hongyu Cui, Changjun Liu, Xiaole Qi, Yanping Zhang, Kai Li, Li Gao, Xiaomei Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	fowl adenovirus 4 hepatitis-hydropericardium syndrome rHN20 live vaccine immunogenicity evaluation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c1717a9fe6d6434280e78938e4bebd8f
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spelling	oai:doaj.org-article:c1717a9fe6d6434280e78938e4bebd8f2021-11-25T19:12:51ZImmunogenicity of Novel Live Vaccine Based on an Artificial rHN20 Strain against Emerging Fowl Adenovirus 410.3390/v131121531999-4915https://doaj.org/article/c1717a9fe6d6434280e78938e4bebd8f2021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2153https://doaj.org/toc/1999-4915In recent years, hepatitis-hydropericardium syndrome (HHS), caused by novel fowl adenovirus 4 (FAdV-4), has caused serious economic losses to the poultry industry. Vaccines are important for preventing and controlling HHS. Current FAdV-4 vaccine research and development are mainly focuses on inactivated vaccines and relatively fewer live vaccines. We previously demonstrated that the hexon gene is the key gene responsible for the high pathogenicity of FAdV-4 and constructed a non-pathogenic chimeric virus rHN20 strain based on the emerging FAdV-4. In this study, the immunogenicity of artificially rescued rHN20 was evaluated in chickens using different routes and doses as a live vaccine. The live rHN20 vaccine induced high titers of neutralizing antibodies against FAdV-4 and fully protected the immunized chickens against a lethal dose of FAdV-4. Furthermore, immunized chickens showed no clinical symptoms or histopathological changes in the FAdV-4-targeted liver, and the viral load in the tissues of immunized chickens was significantly lower than that of chickens in the challenge control group. Collectively, the live rHN20 vaccine effectively protected our sample against FAdV-4 infection and can be considered a live vaccine candidate for preventing HHS in the poultry industry.Yu ZhangQing PanRongrong GuoAijing LiuZhuangzhuang XuYulong GaoHongyu CuiChangjun LiuXiaole QiYanping ZhangKai LiLi GaoXiaomei WangMDPI AGarticlefowl adenovirus 4hepatitis-hydropericardium syndromerHN20live vaccineimmunogenicity evaluationMicrobiologyQR1-502ENViruses, Vol 13, Iss 2153, p 2153 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fowl adenovirus 4 hepatitis-hydropericardium syndrome rHN20 live vaccine immunogenicity evaluation Microbiology QR1-502
spellingShingle	fowl adenovirus 4 hepatitis-hydropericardium syndrome rHN20 live vaccine immunogenicity evaluation Microbiology QR1-502 Yu Zhang Qing Pan Rongrong Guo Aijing Liu Zhuangzhuang Xu Yulong Gao Hongyu Cui Changjun Liu Xiaole Qi Yanping Zhang Kai Li Li Gao Xiaomei Wang Immunogenicity of Novel Live Vaccine Based on an Artificial rHN20 Strain against Emerging Fowl Adenovirus 4
description	In recent years, hepatitis-hydropericardium syndrome (HHS), caused by novel fowl adenovirus 4 (FAdV-4), has caused serious economic losses to the poultry industry. Vaccines are important for preventing and controlling HHS. Current FAdV-4 vaccine research and development are mainly focuses on inactivated vaccines and relatively fewer live vaccines. We previously demonstrated that the hexon gene is the key gene responsible for the high pathogenicity of FAdV-4 and constructed a non-pathogenic chimeric virus rHN20 strain based on the emerging FAdV-4. In this study, the immunogenicity of artificially rescued rHN20 was evaluated in chickens using different routes and doses as a live vaccine. The live rHN20 vaccine induced high titers of neutralizing antibodies against FAdV-4 and fully protected the immunized chickens against a lethal dose of FAdV-4. Furthermore, immunized chickens showed no clinical symptoms or histopathological changes in the FAdV-4-targeted liver, and the viral load in the tissues of immunized chickens was significantly lower than that of chickens in the challenge control group. Collectively, the live rHN20 vaccine effectively protected our sample against FAdV-4 infection and can be considered a live vaccine candidate for preventing HHS in the poultry industry.
format	article
author	Yu Zhang Qing Pan Rongrong Guo Aijing Liu Zhuangzhuang Xu Yulong Gao Hongyu Cui Changjun Liu Xiaole Qi Yanping Zhang Kai Li Li Gao Xiaomei Wang
author_facet	Yu Zhang Qing Pan Rongrong Guo Aijing Liu Zhuangzhuang Xu Yulong Gao Hongyu Cui Changjun Liu Xiaole Qi Yanping Zhang Kai Li Li Gao Xiaomei Wang
author_sort	Yu Zhang
title	Immunogenicity of Novel Live Vaccine Based on an Artificial rHN20 Strain against Emerging Fowl Adenovirus 4
title_short	Immunogenicity of Novel Live Vaccine Based on an Artificial rHN20 Strain against Emerging Fowl Adenovirus 4
title_full	Immunogenicity of Novel Live Vaccine Based on an Artificial rHN20 Strain against Emerging Fowl Adenovirus 4
title_fullStr	Immunogenicity of Novel Live Vaccine Based on an Artificial rHN20 Strain against Emerging Fowl Adenovirus 4
title_full_unstemmed	Immunogenicity of Novel Live Vaccine Based on an Artificial rHN20 Strain against Emerging Fowl Adenovirus 4
title_sort	immunogenicity of novel live vaccine based on an artificial rhn20 strain against emerging fowl adenovirus 4
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c1717a9fe6d6434280e78938e4bebd8f
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Immunogenicity of Novel Live Vaccine Based on an Artificial rHN20 Strain against Emerging Fowl Adenovirus 4

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