Anti-inflammatory and immunomodulating effects of the bacterial lysate in the <em>in vivo</em> models of aseptic lymphadenitis and pneumococcal pneumonia
Bacterial lysates may produce immunoregulatory effects in the inflammatory diseases that are not directly caused by infectious agents; they may also stimulate the immune response against pathogens which are not a part of the lysate composition. Imudon® is a polyvalent bacterial lysate that is availa...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | RU |
| Publicado: |
SPb RAACI
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/c1754802e7be4e49aaa5e23426d4a1a3 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:c1754802e7be4e49aaa5e23426d4a1a3 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:c1754802e7be4e49aaa5e23426d4a1a32021-11-18T08:03:49ZAnti-inflammatory and immunomodulating effects of the bacterial lysate in the <em>in vivo</em> models of aseptic lymphadenitis and pneumococcal pneumonia1563-06252313-741X10.15789/1563-0625-AAI-1758https://doaj.org/article/c1754802e7be4e49aaa5e23426d4a1a32020-01-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1758https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XBacterial lysates may produce immunoregulatory effects in the inflammatory diseases that are not directly caused by infectious agents; they may also stimulate the immune response against pathogens which are not a part of the lysate composition. Imudon® is a polyvalent bacterial lysate that is available in orodispersible tablets. However, the influence of this drug product on aseptic inflammation and immune defense against the infectious agents, the antigens of which are not contained in this preparation have not been studied so far. The aim of this study, therefore, was to determine the anti-inflammatory and immunomodulating effects of Imudon® using the models of aseptic lymphadenitis (in Wistar rats) and pneumococcal pneumonia (in Balb/c mice), i.e., the conditions not related to the specific components of the bacterial lysate. Lymphadenitis was induced in rats by administration of λ-carrageenan into a cervical lymph node via an open operative approach. Whereas pneumonia was induced in mice by administering Streptococcus pneumoniae suspension intranasally. The choice of pneumococcus was determined by the absence of pneumococcal antigens in Imudon®, i.e., it cannot be a direct inducer of adaptive immune response against pneumococcal infection. Imudon® was administered intragastrically as a crushed tablet suspension following a therapeutic-preventive regimen (for 14 days daily until the induction of inflammation and for 3 [in the lymphadenitis model] or 5 days [in the model of pneumonia] in three doses thereafter). In the lymphadenitis model, Imudon® demonstrated both local and systemic anti-inflammatory responses manifested in the reduced number of circulating leucocytes and lower TNFα levels and by ameliorated histological features of inflammation in the operated lymph node. In rats, the anti-inflammatory effect was most pronounced when the product was administered at a dose of 2.2 mg/kg (equivalent to a human therapeutic dose) and 6.6 mg/kg. In the model of pneumonia, administration of Imudon® at 4.44 mg/kg (equivalent to a human therapeutic dose) and 13.32 mg/kg demonstrated a trend towards increased survival rate as compared to the control group. On Day 5 after infection Imudon® (4.44 and 13.32 mg/kg) decreased significantly the severity of inflammation and bacterial titer in the lungs. The titer of anti-pneumococcal immunoglobulins A in the bronchoalveolar lavage fluid were found to be higher in the Imudon® treated group (13.32 mg/kg) compared to control group. The results of this study showed high antiinflammatory and immunomodulatory activities of Imudon® and provided an insight into the mechanisms that underlie the clinical effects of this drug in various inflammatory diseases.K. L. KryshenA. E. KukharenkoA. S. VichareE. A. GaidaiA. A. KryshenYa. A. GushchinO. V. KalyuzhinM. N. MakarovaV. G. MakarovB. MahadevanSPb RAACIarticlebacterial lysatesimudon®immunostimulationimmunoregulationaseptic lymphadenitisinflammationtumor necrosis factorstreptococcus pneumoniaepneumoniaimmunoglobulin aImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 22, Iss 1, Pp 111-122 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
RU |
| topic |
bacterial lysates imudon® immunostimulation immunoregulation aseptic lymphadenitis inflammation tumor necrosis factor streptococcus pneumoniae pneumonia immunoglobulin a Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
bacterial lysates imudon® immunostimulation immunoregulation aseptic lymphadenitis inflammation tumor necrosis factor streptococcus pneumoniae pneumonia immunoglobulin a Immunologic diseases. Allergy RC581-607 K. L. Kryshen A. E. Kukharenko A. S. Vichare E. A. Gaidai A. A. Kryshen Ya. A. Gushchin O. V. Kalyuzhin M. N. Makarova V. G. Makarov B. Mahadevan Anti-inflammatory and immunomodulating effects of the bacterial lysate in the <em>in vivo</em> models of aseptic lymphadenitis and pneumococcal pneumonia |
| description |
Bacterial lysates may produce immunoregulatory effects in the inflammatory diseases that are not directly caused by infectious agents; they may also stimulate the immune response against pathogens which are not a part of the lysate composition. Imudon® is a polyvalent bacterial lysate that is available in orodispersible tablets. However, the influence of this drug product on aseptic inflammation and immune defense against the infectious agents, the antigens of which are not contained in this preparation have not been studied so far. The aim of this study, therefore, was to determine the anti-inflammatory and immunomodulating effects of Imudon® using the models of aseptic lymphadenitis (in Wistar rats) and pneumococcal pneumonia (in Balb/c mice), i.e., the conditions not related to the specific components of the bacterial lysate. Lymphadenitis was induced in rats by administration of λ-carrageenan into a cervical lymph node via an open operative approach. Whereas pneumonia was induced in mice by administering Streptococcus pneumoniae suspension intranasally. The choice of pneumococcus was determined by the absence of pneumococcal antigens in Imudon®, i.e., it cannot be a direct inducer of adaptive immune response against pneumococcal infection. Imudon® was administered intragastrically as a crushed tablet suspension following a therapeutic-preventive regimen (for 14 days daily until the induction of inflammation and for 3 [in the lymphadenitis model] or 5 days [in the model of pneumonia] in three doses thereafter). In the lymphadenitis model, Imudon® demonstrated both local and systemic anti-inflammatory responses manifested in the reduced number of circulating leucocytes and lower TNFα levels and by ameliorated histological features of inflammation in the operated lymph node. In rats, the anti-inflammatory effect was most pronounced when the product was administered at a dose of 2.2 mg/kg (equivalent to a human therapeutic dose) and 6.6 mg/kg. In the model of pneumonia, administration of Imudon® at 4.44 mg/kg (equivalent to a human therapeutic dose) and 13.32 mg/kg demonstrated a trend towards increased survival rate as compared to the control group. On Day 5 after infection Imudon® (4.44 and 13.32 mg/kg) decreased significantly the severity of inflammation and bacterial titer in the lungs. The titer of anti-pneumococcal immunoglobulins A in the bronchoalveolar lavage fluid were found to be higher in the Imudon® treated group (13.32 mg/kg) compared to control group. The results of this study showed high antiinflammatory and immunomodulatory activities of Imudon® and provided an insight into the mechanisms that underlie the clinical effects of this drug in various inflammatory diseases. |
| format |
article |
| author |
K. L. Kryshen A. E. Kukharenko A. S. Vichare E. A. Gaidai A. A. Kryshen Ya. A. Gushchin O. V. Kalyuzhin M. N. Makarova V. G. Makarov B. Mahadevan |
| author_facet |
K. L. Kryshen A. E. Kukharenko A. S. Vichare E. A. Gaidai A. A. Kryshen Ya. A. Gushchin O. V. Kalyuzhin M. N. Makarova V. G. Makarov B. Mahadevan |
| author_sort |
K. L. Kryshen |
| title |
Anti-inflammatory and immunomodulating effects of the bacterial lysate in the <em>in vivo</em> models of aseptic lymphadenitis and pneumococcal pneumonia |
| title_short |
Anti-inflammatory and immunomodulating effects of the bacterial lysate in the <em>in vivo</em> models of aseptic lymphadenitis and pneumococcal pneumonia |
| title_full |
Anti-inflammatory and immunomodulating effects of the bacterial lysate in the <em>in vivo</em> models of aseptic lymphadenitis and pneumococcal pneumonia |
| title_fullStr |
Anti-inflammatory and immunomodulating effects of the bacterial lysate in the <em>in vivo</em> models of aseptic lymphadenitis and pneumococcal pneumonia |
| title_full_unstemmed |
Anti-inflammatory and immunomodulating effects of the bacterial lysate in the <em>in vivo</em> models of aseptic lymphadenitis and pneumococcal pneumonia |
| title_sort |
anti-inflammatory and immunomodulating effects of the bacterial lysate in the <em>in vivo</em> models of aseptic lymphadenitis and pneumococcal pneumonia |
| publisher |
SPb RAACI |
| publishDate |
2020 |
| url |
https://doaj.org/article/c1754802e7be4e49aaa5e23426d4a1a3 |
| work_keys_str_mv |
AT klkryshen antiinflammatoryandimmunomodulatingeffectsofthebacteriallysateintheeminvivoemmodelsofasepticlymphadenitisandpneumococcalpneumonia AT aekukharenko antiinflammatoryandimmunomodulatingeffectsofthebacteriallysateintheeminvivoemmodelsofasepticlymphadenitisandpneumococcalpneumonia AT asvichare antiinflammatoryandimmunomodulatingeffectsofthebacteriallysateintheeminvivoemmodelsofasepticlymphadenitisandpneumococcalpneumonia AT eagaidai antiinflammatoryandimmunomodulatingeffectsofthebacteriallysateintheeminvivoemmodelsofasepticlymphadenitisandpneumococcalpneumonia AT aakryshen antiinflammatoryandimmunomodulatingeffectsofthebacteriallysateintheeminvivoemmodelsofasepticlymphadenitisandpneumococcalpneumonia AT yaagushchin antiinflammatoryandimmunomodulatingeffectsofthebacteriallysateintheeminvivoemmodelsofasepticlymphadenitisandpneumococcalpneumonia AT ovkalyuzhin antiinflammatoryandimmunomodulatingeffectsofthebacteriallysateintheeminvivoemmodelsofasepticlymphadenitisandpneumococcalpneumonia AT mnmakarova antiinflammatoryandimmunomodulatingeffectsofthebacteriallysateintheeminvivoemmodelsofasepticlymphadenitisandpneumococcalpneumonia AT vgmakarov antiinflammatoryandimmunomodulatingeffectsofthebacteriallysateintheeminvivoemmodelsofasepticlymphadenitisandpneumococcalpneumonia AT bmahadevan antiinflammatoryandimmunomodulatingeffectsofthebacteriallysateintheeminvivoemmodelsofasepticlymphadenitisandpneumococcalpneumonia |
| _version_ |
1718422311697121280 |