Lipopolysaccharide directly inhibits bicarbonate absorption by the renal outer medullary collecting duct

Abstract Acidosis is associated with E. coli induced pyelonephritis but whether bacterial cell wall constituents inhibit HCO3 transport in the outer medullary collecting duct from the inner stripe (OMCDi) is not known. We examined the effect of lipopolysaccharide (LPS), on HCO3 absorption in isolate...

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Autores principales:	Shuichi Tsuruoka, Jeffrey M. Purkerson, George J. Schwartz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c1828bcde7d84522b9099033db3780ac
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spelling	oai:doaj.org-article:c1828bcde7d84522b9099033db3780ac2021-12-02T15:10:25ZLipopolysaccharide directly inhibits bicarbonate absorption by the renal outer medullary collecting duct10.1038/s41598-020-77363-w2045-2322https://doaj.org/article/c1828bcde7d84522b9099033db3780ac2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77363-whttps://doaj.org/toc/2045-2322Abstract Acidosis is associated with E. coli induced pyelonephritis but whether bacterial cell wall constituents inhibit HCO3 transport in the outer medullary collecting duct from the inner stripe (OMCDi) is not known. We examined the effect of lipopolysaccharide (LPS), on HCO3 absorption in isolated perfused rabbit OMCDi. LPS caused a ~ 40% decrease in HCO3 absorption, providing a mechanism for E. coli pyelonephritis-induced acidosis. Monophosphoryl lipid A (MPLA), a detoxified TLR4 agonist, and Wortmannin, a phosphoinositide 3-kinase inhibitor, prevented the LPS-mediated decrease, demonstrating the role of TLR4-PI3-kinase signaling and providing proof-of-concept for therapeutic interventions aimed at ameliorating OMCDi dysfunction and pyelonephritis-induced acidosis.Shuichi TsuruokaJeffrey M. PurkersonGeorge J. SchwartzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuichi Tsuruoka Jeffrey M. Purkerson George J. Schwartz Lipopolysaccharide directly inhibits bicarbonate absorption by the renal outer medullary collecting duct
description	Abstract Acidosis is associated with E. coli induced pyelonephritis but whether bacterial cell wall constituents inhibit HCO3 transport in the outer medullary collecting duct from the inner stripe (OMCDi) is not known. We examined the effect of lipopolysaccharide (LPS), on HCO3 absorption in isolated perfused rabbit OMCDi. LPS caused a ~ 40% decrease in HCO3 absorption, providing a mechanism for E. coli pyelonephritis-induced acidosis. Monophosphoryl lipid A (MPLA), a detoxified TLR4 agonist, and Wortmannin, a phosphoinositide 3-kinase inhibitor, prevented the LPS-mediated decrease, demonstrating the role of TLR4-PI3-kinase signaling and providing proof-of-concept for therapeutic interventions aimed at ameliorating OMCDi dysfunction and pyelonephritis-induced acidosis.
format	article
author	Shuichi Tsuruoka Jeffrey M. Purkerson George J. Schwartz
author_facet	Shuichi Tsuruoka Jeffrey M. Purkerson George J. Schwartz
author_sort	Shuichi Tsuruoka
title	Lipopolysaccharide directly inhibits bicarbonate absorption by the renal outer medullary collecting duct
title_short	Lipopolysaccharide directly inhibits bicarbonate absorption by the renal outer medullary collecting duct
title_full	Lipopolysaccharide directly inhibits bicarbonate absorption by the renal outer medullary collecting duct
title_fullStr	Lipopolysaccharide directly inhibits bicarbonate absorption by the renal outer medullary collecting duct
title_full_unstemmed	Lipopolysaccharide directly inhibits bicarbonate absorption by the renal outer medullary collecting duct
title_sort	lipopolysaccharide directly inhibits bicarbonate absorption by the renal outer medullary collecting duct
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c1828bcde7d84522b9099033db3780ac
work_keys_str_mv	AT shuichitsuruoka lipopolysaccharidedirectlyinhibitsbicarbonateabsorptionbytherenaloutermedullarycollectingduct AT jeffreympurkerson lipopolysaccharidedirectlyinhibitsbicarbonateabsorptionbytherenaloutermedullarycollectingduct AT georgejschwartz lipopolysaccharidedirectlyinhibitsbicarbonateabsorptionbytherenaloutermedullarycollectingduct
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Lipopolysaccharide directly inhibits bicarbonate absorption by the renal outer medullary collecting duct

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