Production of bacterial cellulose using Gluconacetobacter kombuchae immobilized on Luffa aegyptiaca support

Abstract The present work report for the first time on the production of bacterial cellulose (BC) using natural loofa sponge (Luffa aegyptiaca) as a scaffold for the immobilization of Gluconacetobacter kombuchae. Bacterial cellulose (BC) are recently gained more attention in several fields including...

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Autores principales:	Sameeha Syed Abdul Rahman, T. Vaishnavi, G. Sai Vidyasri, K. Sathya, P. Priyanka, Ponnusami Venkatachalam, Sugumaran Karuppiah
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c18305ba7c554e74b4d92c03d00e8695
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spelling	oai:doaj.org-article:c18305ba7c554e74b4d92c03d00e86952021-12-02T14:06:25ZProduction of bacterial cellulose using Gluconacetobacter kombuchae immobilized on Luffa aegyptiaca support10.1038/s41598-021-82596-42045-2322https://doaj.org/article/c18305ba7c554e74b4d92c03d00e86952021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82596-4https://doaj.org/toc/2045-2322Abstract The present work report for the first time on the production of bacterial cellulose (BC) using natural loofa sponge (Luffa aegyptiaca) as a scaffold for the immobilization of Gluconacetobacter kombuchae. Bacterial cellulose (BC) are recently gained more attention in several fields including biological and biomedical applications due to their outstanding physico-chemical characteristics including high thermal stability, easy biodegradability, good water holding capacity, high tensile strength, and high degree of polymerization. The increase in requirement of alternative method for the enhancement of BC production under economical aspect develops a positive impact in large scale industries. In this study, Luffa aegyptiaca (LA) was introduced in a separate fermentation medium so as to enhance the concentration of BC production by Gluconacetobacter kombuchae. Different process/medium parameters such as initial pH, static/shaking condition, inoculum size, nitrogen source, C/N ratio, supplements (ethanol and acetic acid) were analysed for the production of bacterial cellulose using LA support. The maximum yield of BC was obtained using following condition: culturing condition -shaking; initial pH − 5.5; nitrogen source- yeast extract, C/N ratio – 40 and supplement—ethanol. The characterization of the BC was examined using Fourier Transform Infra-Red spectroscopy and thermo gravimetric analysis. The biofilm formation on the surface of LA was examined by SEM photographs. Thus, implementation of LA as a support in shaking fermentation under suitable medium/process variables enhanced the BC production.Sameeha Syed Abdul RahmanT. VaishnaviG. Sai VidyasriK. SathyaP. PriyankaPonnusami VenkatachalamSugumaran KaruppiahNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sameeha Syed Abdul Rahman T. Vaishnavi G. Sai Vidyasri K. Sathya P. Priyanka Ponnusami Venkatachalam Sugumaran Karuppiah Production of bacterial cellulose using Gluconacetobacter kombuchae immobilized on Luffa aegyptiaca support
description	Abstract The present work report for the first time on the production of bacterial cellulose (BC) using natural loofa sponge (Luffa aegyptiaca) as a scaffold for the immobilization of Gluconacetobacter kombuchae. Bacterial cellulose (BC) are recently gained more attention in several fields including biological and biomedical applications due to their outstanding physico-chemical characteristics including high thermal stability, easy biodegradability, good water holding capacity, high tensile strength, and high degree of polymerization. The increase in requirement of alternative method for the enhancement of BC production under economical aspect develops a positive impact in large scale industries. In this study, Luffa aegyptiaca (LA) was introduced in a separate fermentation medium so as to enhance the concentration of BC production by Gluconacetobacter kombuchae. Different process/medium parameters such as initial pH, static/shaking condition, inoculum size, nitrogen source, C/N ratio, supplements (ethanol and acetic acid) were analysed for the production of bacterial cellulose using LA support. The maximum yield of BC was obtained using following condition: culturing condition -shaking; initial pH − 5.5; nitrogen source- yeast extract, C/N ratio – 40 and supplement—ethanol. The characterization of the BC was examined using Fourier Transform Infra-Red spectroscopy and thermo gravimetric analysis. The biofilm formation on the surface of LA was examined by SEM photographs. Thus, implementation of LA as a support in shaking fermentation under suitable medium/process variables enhanced the BC production.
format	article
author	Sameeha Syed Abdul Rahman T. Vaishnavi G. Sai Vidyasri K. Sathya P. Priyanka Ponnusami Venkatachalam Sugumaran Karuppiah
author_facet	Sameeha Syed Abdul Rahman T. Vaishnavi G. Sai Vidyasri K. Sathya P. Priyanka Ponnusami Venkatachalam Sugumaran Karuppiah
author_sort	Sameeha Syed Abdul Rahman
title	Production of bacterial cellulose using Gluconacetobacter kombuchae immobilized on Luffa aegyptiaca support
title_short	Production of bacterial cellulose using Gluconacetobacter kombuchae immobilized on Luffa aegyptiaca support
title_full	Production of bacterial cellulose using Gluconacetobacter kombuchae immobilized on Luffa aegyptiaca support
title_fullStr	Production of bacterial cellulose using Gluconacetobacter kombuchae immobilized on Luffa aegyptiaca support
title_full_unstemmed	Production of bacterial cellulose using Gluconacetobacter kombuchae immobilized on Luffa aegyptiaca support
title_sort	production of bacterial cellulose using gluconacetobacter kombuchae immobilized on luffa aegyptiaca support
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c18305ba7c554e74b4d92c03d00e8695
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Production of bacterial cellulose using Gluconacetobacter kombuchae immobilized on Luffa aegyptiaca support

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