Analysis of Static Molecular Gradients in a High-Throughput Drug Screening Microfluidic Assay

In this study, we thoroughly analyzed molecular gradient generation, its stability over time, and linearity in our high-throughput drug screening microfluidic assay (HTS). These parameters greatly affect the precision and accuracy of the device’s analytical protocol. As part of the research, we deve...

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Autores principales:	Roman G. Szafran, Benita Wiatrak
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	HTS lab-on-chip cell culture biochip tumor-on-a-chip microfluidics Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/c186f3c2db3e44af82c41ed8ac9e58de
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spelling	oai:doaj.org-article:c186f3c2db3e44af82c41ed8ac9e58de2021-11-11T18:24:40ZAnalysis of Static Molecular Gradients in a High-Throughput Drug Screening Microfluidic Assay10.3390/molecules262163851420-3049https://doaj.org/article/c186f3c2db3e44af82c41ed8ac9e58de2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6385https://doaj.org/toc/1420-3049In this study, we thoroughly analyzed molecular gradient generation, its stability over time, and linearity in our high-throughput drug screening microfluidic assay (HTS). These parameters greatly affect the precision and accuracy of the device’s analytical protocol. As part of the research, we developed a mathematical model of dependence of the concentration profile on the initial concentrations of active substances in reservoirs and the number of tilts, as well as the dependence of the active substance concentration profiles in the culture chambers on the concentration profile of the reference dye in the indicator chamber. The mean concentration prediction error of the proposed equations ranged from 1.4% to 2.4% for the optimized parameters of the procedure and did not increase with the incubation time. The concentration profile linearity index, Pearson’s correlation coefficient reached −0.997 for 25 device tilts. The observed time stability of the profiles was very good. The mean difference between the concentration profile after 5 days of incubation and the baseline profile was only 7.0%. The newly created mathematical relationships became part of the new HTS biochip operating protocols, which are detailed in the article.Roman G. SzafranBenita WiatrakMDPI AGarticleHTSlab-on-chipcell culturebiochiptumor-on-a-chipmicrofluidicsOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6385, p 6385 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HTS lab-on-chip cell culture biochip tumor-on-a-chip microfluidics Organic chemistry QD241-441
spellingShingle	HTS lab-on-chip cell culture biochip tumor-on-a-chip microfluidics Organic chemistry QD241-441 Roman G. Szafran Benita Wiatrak Analysis of Static Molecular Gradients in a High-Throughput Drug Screening Microfluidic Assay
description	In this study, we thoroughly analyzed molecular gradient generation, its stability over time, and linearity in our high-throughput drug screening microfluidic assay (HTS). These parameters greatly affect the precision and accuracy of the device’s analytical protocol. As part of the research, we developed a mathematical model of dependence of the concentration profile on the initial concentrations of active substances in reservoirs and the number of tilts, as well as the dependence of the active substance concentration profiles in the culture chambers on the concentration profile of the reference dye in the indicator chamber. The mean concentration prediction error of the proposed equations ranged from 1.4% to 2.4% for the optimized parameters of the procedure and did not increase with the incubation time. The concentration profile linearity index, Pearson’s correlation coefficient reached −0.997 for 25 device tilts. The observed time stability of the profiles was very good. The mean difference between the concentration profile after 5 days of incubation and the baseline profile was only 7.0%. The newly created mathematical relationships became part of the new HTS biochip operating protocols, which are detailed in the article.
format	article
author	Roman G. Szafran Benita Wiatrak
author_facet	Roman G. Szafran Benita Wiatrak
author_sort	Roman G. Szafran
title	Analysis of Static Molecular Gradients in a High-Throughput Drug Screening Microfluidic Assay
title_short	Analysis of Static Molecular Gradients in a High-Throughput Drug Screening Microfluidic Assay
title_full	Analysis of Static Molecular Gradients in a High-Throughput Drug Screening Microfluidic Assay
title_fullStr	Analysis of Static Molecular Gradients in a High-Throughput Drug Screening Microfluidic Assay
title_full_unstemmed	Analysis of Static Molecular Gradients in a High-Throughput Drug Screening Microfluidic Assay
title_sort	analysis of static molecular gradients in a high-throughput drug screening microfluidic assay
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c186f3c2db3e44af82c41ed8ac9e58de
work_keys_str_mv	AT romangszafran analysisofstaticmoleculargradientsinahighthroughputdrugscreeningmicrofluidicassay AT benitawiatrak analysisofstaticmoleculargradientsinahighthroughputdrugscreeningmicrofluidicassay
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Analysis of Static Molecular Gradients in a High-Throughput Drug Screening Microfluidic Assay

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