Naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells

Huey-Ming Lo,1,2 Ming-Chieh Ma,1 Jiunn-Min Shieh,3,4 Hui-Ling Chen,5 Wen-Bin Wu1 1School of Medicine, Fu-Jen Catholic University, New Taipei City, Taiwan; 2Section of Cardiology, Department of Internal Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan; 3Department of Internal Medicine,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lo HM, Ma MC, Shieh JM, Chen HL, Wu WB
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	adhesion AuNP nanoparticle migration platelet-derived growth factor VSMC. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c18e84eb3f484cd4aaa07ae0492ce85d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c18e84eb3f484cd4aaa07ae0492ce85d
record_format	dspace
spelling	oai:doaj.org-article:c18e84eb3f484cd4aaa07ae0492ce85d2021-12-02T02:42:28ZNaked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells1178-2013https://doaj.org/article/c18e84eb3f484cd4aaa07ae0492ce85d2018-05-01T00:00:00Zhttps://www.dovepress.com/naked-physically-synthesized-gold-nanoparticles-affect-migration-mitoc-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Huey-Ming Lo,1,2 Ming-Chieh Ma,1 Jiunn-Min Shieh,3,4 Hui-Ling Chen,5 Wen-Bin Wu1 1School of Medicine, Fu-Jen Catholic University, New Taipei City, Taiwan; 2Section of Cardiology, Department of Internal Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan; 3Department of Internal Medicine, Chi-Mei Medical Center, Tainan, Taiwan; 4Department of Recreation and Healthcare Management, Chia Nan University of Pharmacy and Science, Tainan, Taiwan; 5Holistic Education Center, Fu-Jen Catholic University, New Taipei City, Taiwan Introduction: Vascular smooth muscle cells (VSMCs) play an important role in the development and progression of atherosclerosis and vascular injuries in terms of proliferation and migration. Therefore, the aim of this study was to investigate the anti-migratory and proliferative effects of naked gold nanoparticles (AuNPs) on VSMCs. Materials and methods: One set of physically synthesized AuNPs (pAuNPs) and three sets of chemically synthesized AuNPs (cAuNPs) were tested. Results and discussion: Among them, the pAuNPs were found to significantly and markedly inhibit platelet-derived growth factor (PDGF)-induced VSMC migration. Transmission electron microscopy revealed that the pAuNPs were ingested and aggregated in the cytoplasm at an early stage of treatment, while the viability of VSMCs was not affected within 24 hours of treatment. The pAuNP treatment enhanced cellular mitochondrial activity but inhibited basal and PDGF-induced VSMC proliferation, as determined by MTT, WST-1, and BrdU cell proliferation assays. Furthermore, the pAuNPs did not interfere with PDGF signaling or matrix metalloproteinase-2 expression/activity. Unlike the cAuNPs, the pAuNPs could markedly reduce VSMC adhesion to collagen, which was supported by the findings that the pAuNPs could inhibit collagen-induced tyrosine protein and focal adhesion kinase (FAK) phosphorylation and actin cytoskeleton reorganization during cell adhesion. The in vitro effects of the pAuNPs were confirmed in the in vivo rat balloon-injured carotid artery model by diminishing the proliferating VSMCs. Conclusion: Taken together, the present study provides the first evidence that naked pAuNPs can reduce VSMC migration and compromise cell adhesion by affecting FAK and tyrosine-protein activation. The pAuNPs also have an inhibitory effect on PDGF-induced VSMC proliferation and can reduce proliferating/migrating VSMC expression in vivo. Keywords: adhesion, AuNP, cardiovascular disease, FAK, platelet-derived growth factor, VSMC, TEMLo HMMa MCShieh JMChen HLWu WBDove Medical PressarticleadhesionAuNPnanoparticlemigrationplatelet-derived growth factorVSMC.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 3163-3176 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	adhesion AuNP nanoparticle migration platelet-derived growth factor VSMC. Medicine (General) R5-920
spellingShingle	adhesion AuNP nanoparticle migration platelet-derived growth factor VSMC. Medicine (General) R5-920 Lo HM Ma MC Shieh JM Chen HL Wu WB Naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells
description	Huey-Ming Lo,1,2 Ming-Chieh Ma,1 Jiunn-Min Shieh,3,4 Hui-Ling Chen,5 Wen-Bin Wu1 1School of Medicine, Fu-Jen Catholic University, New Taipei City, Taiwan; 2Section of Cardiology, Department of Internal Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan; 3Department of Internal Medicine, Chi-Mei Medical Center, Tainan, Taiwan; 4Department of Recreation and Healthcare Management, Chia Nan University of Pharmacy and Science, Tainan, Taiwan; 5Holistic Education Center, Fu-Jen Catholic University, New Taipei City, Taiwan Introduction: Vascular smooth muscle cells (VSMCs) play an important role in the development and progression of atherosclerosis and vascular injuries in terms of proliferation and migration. Therefore, the aim of this study was to investigate the anti-migratory and proliferative effects of naked gold nanoparticles (AuNPs) on VSMCs. Materials and methods: One set of physically synthesized AuNPs (pAuNPs) and three sets of chemically synthesized AuNPs (cAuNPs) were tested. Results and discussion: Among them, the pAuNPs were found to significantly and markedly inhibit platelet-derived growth factor (PDGF)-induced VSMC migration. Transmission electron microscopy revealed that the pAuNPs were ingested and aggregated in the cytoplasm at an early stage of treatment, while the viability of VSMCs was not affected within 24 hours of treatment. The pAuNP treatment enhanced cellular mitochondrial activity but inhibited basal and PDGF-induced VSMC proliferation, as determined by MTT, WST-1, and BrdU cell proliferation assays. Furthermore, the pAuNPs did not interfere with PDGF signaling or matrix metalloproteinase-2 expression/activity. Unlike the cAuNPs, the pAuNPs could markedly reduce VSMC adhesion to collagen, which was supported by the findings that the pAuNPs could inhibit collagen-induced tyrosine protein and focal adhesion kinase (FAK) phosphorylation and actin cytoskeleton reorganization during cell adhesion. The in vitro effects of the pAuNPs were confirmed in the in vivo rat balloon-injured carotid artery model by diminishing the proliferating VSMCs. Conclusion: Taken together, the present study provides the first evidence that naked pAuNPs can reduce VSMC migration and compromise cell adhesion by affecting FAK and tyrosine-protein activation. The pAuNPs also have an inhibitory effect on PDGF-induced VSMC proliferation and can reduce proliferating/migrating VSMC expression in vivo. Keywords: adhesion, AuNP, cardiovascular disease, FAK, platelet-derived growth factor, VSMC, TEM
format	article
author	Lo HM Ma MC Shieh JM Chen HL Wu WB
author_facet	Lo HM Ma MC Shieh JM Chen HL Wu WB
author_sort	Lo HM
title	Naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells
title_short	Naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells
title_full	Naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells
title_fullStr	Naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells
title_full_unstemmed	Naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells
title_sort	naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/c18e84eb3f484cd4aaa07ae0492ce85d
work_keys_str_mv	AT lohm nakedphysicallysynthesizedgoldnanoparticlesaffectmigrationmitochondrialactivityandproliferationofvascularsmoothmusclecells AT mamc nakedphysicallysynthesizedgoldnanoparticlesaffectmigrationmitochondrialactivityandproliferationofvascularsmoothmusclecells AT shiehjm nakedphysicallysynthesizedgoldnanoparticlesaffectmigrationmitochondrialactivityandproliferationofvascularsmoothmusclecells AT chenhl nakedphysicallysynthesizedgoldnanoparticlesaffectmigrationmitochondrialactivityandproliferationofvascularsmoothmusclecells AT wuwb nakedphysicallysynthesizedgoldnanoparticlesaffectmigrationmitochondrialactivityandproliferationofvascularsmoothmusclecells
_version_	1718402248187314176

Naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells

Ejemplares similares