Morphology, distribution and phenotype of polycystin kidney disease 2-like 1-positive cerebrospinal fluid contacting neurons in the brainstem of adult mice.

The mammalian spinal cord and medulla oblongata harbor unique neurons that remain in contact with the cerebrospinal fluid (CSF-cNs). These neurons were shown recently to express a polycystin member of the TRP channels family (PKD2L1) that potentially acts as a chemo- or mechanoreceptor. Recent studi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Adeline Orts-Del'Immagine, Anne Kastner, Vanessa Tillement, Catherine Tardivel, Jérôme Trouslard, Nicolas Wanaverbecq
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c19c7b023f11431ba5e11f874a0eca8d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c19c7b023f11431ba5e11f874a0eca8d
record_format	dspace
spelling	oai:doaj.org-article:c19c7b023f11431ba5e11f874a0eca8d2021-11-18T08:33:55ZMorphology, distribution and phenotype of polycystin kidney disease 2-like 1-positive cerebrospinal fluid contacting neurons in the brainstem of adult mice.1932-620310.1371/journal.pone.0087748https://doaj.org/article/c19c7b023f11431ba5e11f874a0eca8d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24504595/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The mammalian spinal cord and medulla oblongata harbor unique neurons that remain in contact with the cerebrospinal fluid (CSF-cNs). These neurons were shown recently to express a polycystin member of the TRP channels family (PKD2L1) that potentially acts as a chemo- or mechanoreceptor. Recent studies carried out in young rodents indicate that spinal CSF-cNs express immature neuronal markers that appear to persist even in adult cells. Nevertheless, little is known about the phenotype and morphological properties of medullar CSF-cNs. Using immunohistochemistry and confocal microscopy techniques on tissues obtained from three-month old PKD2L1:EGFP transgenic mice, we analyzed the morphology, distribution, localization and phenotype of PKD2L1(+) CSF-cNs around the brainstem and cervical spinal cord central canal. We show that PKD2L1(+) CSF-cNs are GABAergic neurons with a subependymal localization, projecting a dendrite towards the central canal and an axon-like process running through the parenchyma. These neurons display a primary cilium on the soma and the dendritic process appears to bear ciliary-like structures in contact with the CSF. PKD2L1(+) CSF-cNs present a conserved morphology along the length of the medullospinal central canal with a change in their density, localization and dendritic length according to the rostro-caudal axis. At adult stages, PKD2L1(+) medullar CSF-cNs appear to remain in an intermediate state of maturation since they still exhibit characteristics of neuronal immaturity (DCX positive, neurofilament 160 kDa negative) along with the expression of a marker representative of neuronal maturation (NeuN). In addition, PKD2L1(+) CSF-cNs express Nkx6.1, a homeodomain protein that enables the differentiation of ventral progenitors into somatic motoneurons and interneurons. The present study provides valuable information on the cellular properties of this peculiar neuronal population that will be crucial for understanding the physiological role of CSF-cNs in mammals and their link with the stem cells contained in the region surrounding the medullospinal central canal.Adeline Orts-Del'ImmagineAnne KastnerVanessa TillementCatherine TardivelJérôme TrouslardNicolas WanaverbecqPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e87748 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adeline Orts-Del'Immagine Anne Kastner Vanessa Tillement Catherine Tardivel Jérôme Trouslard Nicolas Wanaverbecq Morphology, distribution and phenotype of polycystin kidney disease 2-like 1-positive cerebrospinal fluid contacting neurons in the brainstem of adult mice.
description	The mammalian spinal cord and medulla oblongata harbor unique neurons that remain in contact with the cerebrospinal fluid (CSF-cNs). These neurons were shown recently to express a polycystin member of the TRP channels family (PKD2L1) that potentially acts as a chemo- or mechanoreceptor. Recent studies carried out in young rodents indicate that spinal CSF-cNs express immature neuronal markers that appear to persist even in adult cells. Nevertheless, little is known about the phenotype and morphological properties of medullar CSF-cNs. Using immunohistochemistry and confocal microscopy techniques on tissues obtained from three-month old PKD2L1:EGFP transgenic mice, we analyzed the morphology, distribution, localization and phenotype of PKD2L1(+) CSF-cNs around the brainstem and cervical spinal cord central canal. We show that PKD2L1(+) CSF-cNs are GABAergic neurons with a subependymal localization, projecting a dendrite towards the central canal and an axon-like process running through the parenchyma. These neurons display a primary cilium on the soma and the dendritic process appears to bear ciliary-like structures in contact with the CSF. PKD2L1(+) CSF-cNs present a conserved morphology along the length of the medullospinal central canal with a change in their density, localization and dendritic length according to the rostro-caudal axis. At adult stages, PKD2L1(+) medullar CSF-cNs appear to remain in an intermediate state of maturation since they still exhibit characteristics of neuronal immaturity (DCX positive, neurofilament 160 kDa negative) along with the expression of a marker representative of neuronal maturation (NeuN). In addition, PKD2L1(+) CSF-cNs express Nkx6.1, a homeodomain protein that enables the differentiation of ventral progenitors into somatic motoneurons and interneurons. The present study provides valuable information on the cellular properties of this peculiar neuronal population that will be crucial for understanding the physiological role of CSF-cNs in mammals and their link with the stem cells contained in the region surrounding the medullospinal central canal.
format	article
author	Adeline Orts-Del'Immagine Anne Kastner Vanessa Tillement Catherine Tardivel Jérôme Trouslard Nicolas Wanaverbecq
author_facet	Adeline Orts-Del'Immagine Anne Kastner Vanessa Tillement Catherine Tardivel Jérôme Trouslard Nicolas Wanaverbecq
author_sort	Adeline Orts-Del'Immagine
title	Morphology, distribution and phenotype of polycystin kidney disease 2-like 1-positive cerebrospinal fluid contacting neurons in the brainstem of adult mice.
title_short	Morphology, distribution and phenotype of polycystin kidney disease 2-like 1-positive cerebrospinal fluid contacting neurons in the brainstem of adult mice.
title_full	Morphology, distribution and phenotype of polycystin kidney disease 2-like 1-positive cerebrospinal fluid contacting neurons in the brainstem of adult mice.
title_fullStr	Morphology, distribution and phenotype of polycystin kidney disease 2-like 1-positive cerebrospinal fluid contacting neurons in the brainstem of adult mice.
title_full_unstemmed	Morphology, distribution and phenotype of polycystin kidney disease 2-like 1-positive cerebrospinal fluid contacting neurons in the brainstem of adult mice.
title_sort	morphology, distribution and phenotype of polycystin kidney disease 2-like 1-positive cerebrospinal fluid contacting neurons in the brainstem of adult mice.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/c19c7b023f11431ba5e11f874a0eca8d
work_keys_str_mv	AT adelineortsdelimmagine morphologydistributionandphenotypeofpolycystinkidneydisease2like1positivecerebrospinalfluidcontactingneuronsinthebrainstemofadultmice AT annekastner morphologydistributionandphenotypeofpolycystinkidneydisease2like1positivecerebrospinalfluidcontactingneuronsinthebrainstemofadultmice AT vanessatillement morphologydistributionandphenotypeofpolycystinkidneydisease2like1positivecerebrospinalfluidcontactingneuronsinthebrainstemofadultmice AT catherinetardivel morphologydistributionandphenotypeofpolycystinkidneydisease2like1positivecerebrospinalfluidcontactingneuronsinthebrainstemofadultmice AT jerometrouslard morphologydistributionandphenotypeofpolycystinkidneydisease2like1positivecerebrospinalfluidcontactingneuronsinthebrainstemofadultmice AT nicolaswanaverbecq morphologydistributionandphenotypeofpolycystinkidneydisease2like1positivecerebrospinalfluidcontactingneuronsinthebrainstemofadultmice
_version_	1718421645750697984

Morphology, distribution and phenotype of polycystin kidney disease 2-like 1-positive cerebrospinal fluid contacting neurons in the brainstem of adult mice.

Ejemplares similares