Comprehensive Mapping of the Cell Response to Borrelia bavariensis in the Brain Microvascular Endothelial Cells in vitro Using RNA-Seq

Borrelia bavariensis can invade the central nervous system (CNS) by crossing the blood-brain barrier (BBB). It is predicted that B. bavariensis evokes numerous signaling cascades in the human brain microvascular endothelial cells (hBMECs) and exploits them to traverse across the BBB. The complete pi...

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Autores principales:	Zuzana Tkáčová, Katarína Bhide, Evelina Mochnáčová, Patrícia Petroušková, Jana Hruškovicová, Amod Kulkarni, Mangesh Bhide
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Borrelia bavariensis BBB transcriptom RNA-seq endothelial cells BBB crossing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c1a34debb2b244db89a4e11ddb7e2a73
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spelling	oai:doaj.org-article:c1a34debb2b244db89a4e11ddb7e2a732021-11-08T07:28:40ZComprehensive Mapping of the Cell Response to Borrelia bavariensis in the Brain Microvascular Endothelial Cells in vitro Using RNA-Seq1664-302X10.3389/fmicb.2021.760627https://doaj.org/article/c1a34debb2b244db89a4e11ddb7e2a732021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.760627/fullhttps://doaj.org/toc/1664-302XBorrelia bavariensis can invade the central nervous system (CNS) by crossing the blood-brain barrier (BBB). It is predicted that B. bavariensis evokes numerous signaling cascades in the human brain microvascular endothelial cells (hBMECs) and exploits them to traverse across the BBB. The complete picture of signaling events in hBMECs induced by B. bavariensis remains uncovered. Using RNA sequencing, we mapped 11,398 genes and identified 295 differentially expressed genes (DEGs, 251 upregulated genes and 44 downregulated genes) in B. bavariensis challenged hBMECs. The results obtained from RNA-seq were validated with qPCR. Gene ontology analysis revealed the participation of DEGs in a number of biological processes like cell communication, organization of the extracellular matrix, vesicle-mediated transport, cell response triggered by pattern recognition receptors, antigen processing via MHC class I, cellular stress, metabolism, signal transduction, etc. The expression of several non-protein coding genes was also evoked. In this manuscript, we discuss in detail the correlation between several signaling cascades elicited and the translocation of BBB by B. bavariensis. The data revealed here may contribute to a better understanding of the mechanisms employed by B. bavariensis to cross the BBB.Zuzana TkáčováKatarína BhideEvelina MochnáčováPatrícia PetrouškováJana HruškovicováAmod KulkarniAmod KulkarniMangesh BhideMangesh BhideFrontiers Media S.A.articleBorrelia bavariensisBBBtranscriptomRNA-seqendothelial cellsBBB crossingMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Borrelia bavariensis BBB transcriptom RNA-seq endothelial cells BBB crossing Microbiology QR1-502
spellingShingle	Borrelia bavariensis BBB transcriptom RNA-seq endothelial cells BBB crossing Microbiology QR1-502 Zuzana Tkáčová Katarína Bhide Evelina Mochnáčová Patrícia Petroušková Jana Hruškovicová Amod Kulkarni Amod Kulkarni Mangesh Bhide Mangesh Bhide Comprehensive Mapping of the Cell Response to Borrelia bavariensis in the Brain Microvascular Endothelial Cells in vitro Using RNA-Seq
description	Borrelia bavariensis can invade the central nervous system (CNS) by crossing the blood-brain barrier (BBB). It is predicted that B. bavariensis evokes numerous signaling cascades in the human brain microvascular endothelial cells (hBMECs) and exploits them to traverse across the BBB. The complete picture of signaling events in hBMECs induced by B. bavariensis remains uncovered. Using RNA sequencing, we mapped 11,398 genes and identified 295 differentially expressed genes (DEGs, 251 upregulated genes and 44 downregulated genes) in B. bavariensis challenged hBMECs. The results obtained from RNA-seq were validated with qPCR. Gene ontology analysis revealed the participation of DEGs in a number of biological processes like cell communication, organization of the extracellular matrix, vesicle-mediated transport, cell response triggered by pattern recognition receptors, antigen processing via MHC class I, cellular stress, metabolism, signal transduction, etc. The expression of several non-protein coding genes was also evoked. In this manuscript, we discuss in detail the correlation between several signaling cascades elicited and the translocation of BBB by B. bavariensis. The data revealed here may contribute to a better understanding of the mechanisms employed by B. bavariensis to cross the BBB.
format	article
author	Zuzana Tkáčová Katarína Bhide Evelina Mochnáčová Patrícia Petroušková Jana Hruškovicová Amod Kulkarni Amod Kulkarni Mangesh Bhide Mangesh Bhide
author_facet	Zuzana Tkáčová Katarína Bhide Evelina Mochnáčová Patrícia Petroušková Jana Hruškovicová Amod Kulkarni Amod Kulkarni Mangesh Bhide Mangesh Bhide
author_sort	Zuzana Tkáčová
title	Comprehensive Mapping of the Cell Response to Borrelia bavariensis in the Brain Microvascular Endothelial Cells in vitro Using RNA-Seq
title_short	Comprehensive Mapping of the Cell Response to Borrelia bavariensis in the Brain Microvascular Endothelial Cells in vitro Using RNA-Seq
title_full	Comprehensive Mapping of the Cell Response to Borrelia bavariensis in the Brain Microvascular Endothelial Cells in vitro Using RNA-Seq
title_fullStr	Comprehensive Mapping of the Cell Response to Borrelia bavariensis in the Brain Microvascular Endothelial Cells in vitro Using RNA-Seq
title_full_unstemmed	Comprehensive Mapping of the Cell Response to Borrelia bavariensis in the Brain Microvascular Endothelial Cells in vitro Using RNA-Seq
title_sort	comprehensive mapping of the cell response to borrelia bavariensis in the brain microvascular endothelial cells in vitro using rna-seq
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c1a34debb2b244db89a4e11ddb7e2a73
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Comprehensive Mapping of the Cell Response to Borrelia bavariensis in the Brain Microvascular Endothelial Cells in vitro Using RNA-Seq

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