CYTOKINE EXPRESSION PROFILES OF PERITONEAL FLUID IN THE PATIENTS WITH UTERINE LEIOMYOMA

Abstract. Uterine leiomyoma (UL) is a hormone-dependent benign tumor of uterus. Social significance of UL is stipulated by its high rate among fertile females. Scarce data exist about the impact of cytokines in UL progression. Th1/Th2 paradigm is one of crucial points in modern immunology. Evaluatio...

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Autores principales:	K. A. Syssoev, N. V. Kulagina, Areg A. Totolian
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	uterine leiomyoma cytokines peritoneal fluid Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/c1a7cda0efcd4273b96f1474ae888fa1
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spelling	oai:doaj.org-article:c1a7cda0efcd4273b96f1474ae888fa12021-11-18T08:03:42ZCYTOKINE EXPRESSION PROFILES OF PERITONEAL FLUID IN THE PATIENTS WITH UTERINE LEIOMYOMA1563-06252313-741X10.15789/1563-0625-2006-4-547-556https://doaj.org/article/c1a7cda0efcd4273b96f1474ae888fa12014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/493https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Uterine leiomyoma (UL) is a hormone-dependent benign tumor of uterus. Social significance of UL is stipulated by its high rate among fertile females. Scarce data exist about the impact of cytokines in UL progression. Th1/Th2 paradigm is one of crucial points in modern immunology. Evaluation of cytokines involved into either type of immune response is of special significance for studying the diseases accompanied by the changes of extracellular matrix, e.g., leiomyomas. In present study, we analyzed peritoneal fluids from UL patients, with multiplex detection of IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, GM-CSF, IFN-γ and TNF-α (Th1/Th2 panel), by means of a Bio-Plex® instrument (Bio-Rad, USA). Twenty-seven patients were observed in our study (20 patients with UL, and 7 myoma-free women (a group of comparison). The mean age of the patients was 43.5±0.6 years. The duration of UL ranged from 0 to 18 years. As a result, the levels of IL-10, GM-CSF, IFN-γ and TNF-α in patients with long-existing UL (over 5 years) were significantly higher (p<0,05) than in group with a disease story of <5 years. IFN-γ values in peritoneal fluid patients with UL did inversely correlate with uterine size. Moreover, the levels of IFN-γ in patients with smaller uterine volume (<8 weeks of pregnancy) were increased in relation to the group with larger tumor size. IL-10 contents were increased in the patients with adenomyosis, rapid and slow growth of UL, and in both types of tumor (simple and proliferative). Increased IL-5 levels were observed in the patients with single tumor nodules (as related to the patients bearing multiple nodes, and comparison group). Furthermore, intramural and subserosal location of nodes was characterized by increased levels of IL-5. In the patients free of adenomyosis, IL-5 value was increased against the comparison group. The changes in IL-2, IL-4, IL-12 and IL-13 levels in patients with UL were not statistically significant.K. A. SyssoevN. V. KulaginaAreg A. TotolianSPb RAACIarticleuterine leiomyomacytokinesperitoneal fluidImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 8, Iss 4, Pp 547-556 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	uterine leiomyoma cytokines peritoneal fluid Immunologic diseases. Allergy RC581-607
spellingShingle	uterine leiomyoma cytokines peritoneal fluid Immunologic diseases. Allergy RC581-607 K. A. Syssoev N. V. Kulagina Areg A. Totolian CYTOKINE EXPRESSION PROFILES OF PERITONEAL FLUID IN THE PATIENTS WITH UTERINE LEIOMYOMA
description	Abstract. Uterine leiomyoma (UL) is a hormone-dependent benign tumor of uterus. Social significance of UL is stipulated by its high rate among fertile females. Scarce data exist about the impact of cytokines in UL progression. Th1/Th2 paradigm is one of crucial points in modern immunology. Evaluation of cytokines involved into either type of immune response is of special significance for studying the diseases accompanied by the changes of extracellular matrix, e.g., leiomyomas. In present study, we analyzed peritoneal fluids from UL patients, with multiplex detection of IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, GM-CSF, IFN-γ and TNF-α (Th1/Th2 panel), by means of a Bio-Plex® instrument (Bio-Rad, USA). Twenty-seven patients were observed in our study (20 patients with UL, and 7 myoma-free women (a group of comparison). The mean age of the patients was 43.5±0.6 years. The duration of UL ranged from 0 to 18 years. As a result, the levels of IL-10, GM-CSF, IFN-γ and TNF-α in patients with long-existing UL (over 5 years) were significantly higher (p<0,05) than in group with a disease story of <5 years. IFN-γ values in peritoneal fluid patients with UL did inversely correlate with uterine size. Moreover, the levels of IFN-γ in patients with smaller uterine volume (<8 weeks of pregnancy) were increased in relation to the group with larger tumor size. IL-10 contents were increased in the patients with adenomyosis, rapid and slow growth of UL, and in both types of tumor (simple and proliferative). Increased IL-5 levels were observed in the patients with single tumor nodules (as related to the patients bearing multiple nodes, and comparison group). Furthermore, intramural and subserosal location of nodes was characterized by increased levels of IL-5. In the patients free of adenomyosis, IL-5 value was increased against the comparison group. The changes in IL-2, IL-4, IL-12 and IL-13 levels in patients with UL were not statistically significant.
format	article
author	K. A. Syssoev N. V. Kulagina Areg A. Totolian
author_facet	K. A. Syssoev N. V. Kulagina Areg A. Totolian
author_sort	K. A. Syssoev
title	CYTOKINE EXPRESSION PROFILES OF PERITONEAL FLUID IN THE PATIENTS WITH UTERINE LEIOMYOMA
title_short	CYTOKINE EXPRESSION PROFILES OF PERITONEAL FLUID IN THE PATIENTS WITH UTERINE LEIOMYOMA
title_full	CYTOKINE EXPRESSION PROFILES OF PERITONEAL FLUID IN THE PATIENTS WITH UTERINE LEIOMYOMA
title_fullStr	CYTOKINE EXPRESSION PROFILES OF PERITONEAL FLUID IN THE PATIENTS WITH UTERINE LEIOMYOMA
title_full_unstemmed	CYTOKINE EXPRESSION PROFILES OF PERITONEAL FLUID IN THE PATIENTS WITH UTERINE LEIOMYOMA
title_sort	cytokine expression profiles of peritoneal fluid in the patients with uterine leiomyoma
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/c1a7cda0efcd4273b96f1474ae888fa1
work_keys_str_mv	AT kasyssoev cytokineexpressionprofilesofperitonealfluidinthepatientswithuterineleiomyoma AT nvkulagina cytokineexpressionprofilesofperitonealfluidinthepatientswithuterineleiomyoma AT aregatotolian cytokineexpressionprofilesofperitonealfluidinthepatientswithuterineleiomyoma
_version_	1718422505774907392

CYTOKINE EXPRESSION PROFILES OF PERITONEAL FLUID IN THE PATIENTS WITH UTERINE LEIOMYOMA

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