The Healing Effects of The Topical Mesenchymal Stem Cells Application on Colonic Anastomosis Subjected to Ischemia Reperfusion Injury

Intestinal ischemia reperfusion injury (IRI) is a challenging problem and it adversely affects the healing of colonic anastomosis. Our experimental study aimed to investigate the role of mesenchymal stem cells (MSC) administration in the healing of colonic anastomosis. A total of 33 rats were groupe...

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Autores principales:	Hüseyin ÖZDEN, Gökhan KARACA, Huri BULUT, Mehmet NİYAZ, Muhammed GÖMEÇ, Çağatay E. DAPHAN
Formato:	article
Lenguaje:	EN
Publicado:	University of Kafkas 2021
Materias:	anastomotic leakage colonic anastomosis hydroxyproline i̇schemia reperfusion injury mesenchymal stem cell Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/c1b3af5bd3c844118e709318bcb4601c
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spelling	oai:doaj.org-article:c1b3af5bd3c844118e709318bcb4601c2021-12-01T08:16:03ZThe Healing Effects of The Topical Mesenchymal Stem Cells Application on Colonic Anastomosis Subjected to Ischemia Reperfusion Injury10.9775/kvfd.2021.259351300-60451309-2251https://doaj.org/article/c1b3af5bd3c844118e709318bcb4601c2021-09-01T00:00:00Zhttp://vetdergikafkas.org/uploads/pdf/pdf_KVFD_2831.pdfhttps://doaj.org/toc/1300-6045https://doaj.org/toc/1309-2251Intestinal ischemia reperfusion injury (IRI) is a challenging problem and it adversely affects the healing of colonic anastomosis. Our experimental study aimed to investigate the role of mesenchymal stem cells (MSC) administration in the healing of colonic anastomosis. A total of 33 rats were grouped as Control, IRI and MSC treatment groups. Three rats were reserved for obtaining MSCs. Colonic resection and anastomosis procedure was performed in all groups. Anastomotic line was wrapped with MSCs impregnated spongostan after colonic anastomosis in the rats of the MSC treatment group. All rats were sacrificed and anastomotic line were sampled for examination on the post operative seventh day. Tissue hydroxyproline (HP) levels and anastomotic bursting pressures were statistically compared. Anastomotic bursting pressures were found to be significantly high in MSC treatment group rats. The lowest anastomotic bursting pressure was detected in IRI group rats. Hydroxyproline content of the anastomotic sites were also found to be significantly higher in the rats of the MSC treatment group when compared with the IRI group rats. Our study showed that the detrimental effects of IRI on the healing process of colonic anastomosis in an experimental model may be alleviated with the treatment of MSCs.Hüseyin ÖZDENGökhan KARACAHuri BULUTMehmet NİYAZMuhammed GÖMEÇÇağatay E. DAPHANUniversity of Kafkasarticleanastomotic leakagecolonic anastomosishydroxyprolinei̇schemia reperfusion injurymesenchymal stem cellVeterinary medicineSF600-1100ENKafkas Universitesi Veteriner Fakultesi Dergisi, Vol 27, Iss 5, Pp 595-600 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anastomotic leakage colonic anastomosis hydroxyproline i̇schemia reperfusion injury mesenchymal stem cell Veterinary medicine SF600-1100
spellingShingle	anastomotic leakage colonic anastomosis hydroxyproline i̇schemia reperfusion injury mesenchymal stem cell Veterinary medicine SF600-1100 Hüseyin ÖZDEN Gökhan KARACA Huri BULUT Mehmet NİYAZ Muhammed GÖMEÇ Çağatay E. DAPHAN The Healing Effects of The Topical Mesenchymal Stem Cells Application on Colonic Anastomosis Subjected to Ischemia Reperfusion Injury
description	Intestinal ischemia reperfusion injury (IRI) is a challenging problem and it adversely affects the healing of colonic anastomosis. Our experimental study aimed to investigate the role of mesenchymal stem cells (MSC) administration in the healing of colonic anastomosis. A total of 33 rats were grouped as Control, IRI and MSC treatment groups. Three rats were reserved for obtaining MSCs. Colonic resection and anastomosis procedure was performed in all groups. Anastomotic line was wrapped with MSCs impregnated spongostan after colonic anastomosis in the rats of the MSC treatment group. All rats were sacrificed and anastomotic line were sampled for examination on the post operative seventh day. Tissue hydroxyproline (HP) levels and anastomotic bursting pressures were statistically compared. Anastomotic bursting pressures were found to be significantly high in MSC treatment group rats. The lowest anastomotic bursting pressure was detected in IRI group rats. Hydroxyproline content of the anastomotic sites were also found to be significantly higher in the rats of the MSC treatment group when compared with the IRI group rats. Our study showed that the detrimental effects of IRI on the healing process of colonic anastomosis in an experimental model may be alleviated with the treatment of MSCs.
format	article
author	Hüseyin ÖZDEN Gökhan KARACA Huri BULUT Mehmet NİYAZ Muhammed GÖMEÇ Çağatay E. DAPHAN
author_facet	Hüseyin ÖZDEN Gökhan KARACA Huri BULUT Mehmet NİYAZ Muhammed GÖMEÇ Çağatay E. DAPHAN
author_sort	Hüseyin ÖZDEN
title	The Healing Effects of The Topical Mesenchymal Stem Cells Application on Colonic Anastomosis Subjected to Ischemia Reperfusion Injury
title_short	The Healing Effects of The Topical Mesenchymal Stem Cells Application on Colonic Anastomosis Subjected to Ischemia Reperfusion Injury
title_full	The Healing Effects of The Topical Mesenchymal Stem Cells Application on Colonic Anastomosis Subjected to Ischemia Reperfusion Injury
title_fullStr	The Healing Effects of The Topical Mesenchymal Stem Cells Application on Colonic Anastomosis Subjected to Ischemia Reperfusion Injury
title_full_unstemmed	The Healing Effects of The Topical Mesenchymal Stem Cells Application on Colonic Anastomosis Subjected to Ischemia Reperfusion Injury
title_sort	healing effects of the topical mesenchymal stem cells application on colonic anastomosis subjected to ischemia reperfusion injury
publisher	University of Kafkas
publishDate	2021
url	https://doaj.org/article/c1b3af5bd3c844118e709318bcb4601c
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The Healing Effects of The Topical Mesenchymal Stem Cells Application on Colonic Anastomosis Subjected to Ischemia Reperfusion Injury

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