Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force

Abstract Techniques used to clear biological tissue for fluorescence microscopy are essential to connect anatomical principles at levels ranging from subcellular to the whole animal. Here we report a simple and straightforward approach to efficiently render opaque tissue samples transparent and show...

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Autores principales:	Joseph Dwyer, M. Desmond Ramirez, Paul S. Katz, Rolf O. Karlstrom, Joseph Bergan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c1b507e37c8446c3b4426afebbbe9637
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spelling	oai:doaj.org-article:c1b507e37c8446c3b4426afebbbe96372021-12-02T18:50:53ZAccelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force10.1038/s41598-021-95692-22045-2322https://doaj.org/article/c1b507e37c8446c3b4426afebbbe96372021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95692-2https://doaj.org/toc/2045-2322Abstract Techniques used to clear biological tissue for fluorescence microscopy are essential to connect anatomical principles at levels ranging from subcellular to the whole animal. Here we report a simple and straightforward approach to efficiently render opaque tissue samples transparent and show that this approach can be modified to rapidly label intact tissue samples with antibodies for large volume fluorescence microscopy. This strategy applies a magnetohydrodynamic (MHD) force to accelerate the removal of lipids from tissue samples at least as large as an intact adult mouse brain. We also show that MHD force can be used to accelerate antibody penetration into tissue samples. This strategy complements a growing array of tools that enable high-resolution 3-dimensional anatomical analyses in intact tissues using fluorescence microscopy. MHD-accelerated clearing is simple, fast, reliable, inexpensive, provides good thermal regulation, and is compatible with existing strategies for high-quality fluorescence microscopy of intact tissues.Joseph DwyerM. Desmond RamirezPaul S. KatzRolf O. KarlstromJoseph BerganNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joseph Dwyer M. Desmond Ramirez Paul S. Katz Rolf O. Karlstrom Joseph Bergan Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force
description	Abstract Techniques used to clear biological tissue for fluorescence microscopy are essential to connect anatomical principles at levels ranging from subcellular to the whole animal. Here we report a simple and straightforward approach to efficiently render opaque tissue samples transparent and show that this approach can be modified to rapidly label intact tissue samples with antibodies for large volume fluorescence microscopy. This strategy applies a magnetohydrodynamic (MHD) force to accelerate the removal of lipids from tissue samples at least as large as an intact adult mouse brain. We also show that MHD force can be used to accelerate antibody penetration into tissue samples. This strategy complements a growing array of tools that enable high-resolution 3-dimensional anatomical analyses in intact tissues using fluorescence microscopy. MHD-accelerated clearing is simple, fast, reliable, inexpensive, provides good thermal regulation, and is compatible with existing strategies for high-quality fluorescence microscopy of intact tissues.
format	article
author	Joseph Dwyer M. Desmond Ramirez Paul S. Katz Rolf O. Karlstrom Joseph Bergan
author_facet	Joseph Dwyer M. Desmond Ramirez Paul S. Katz Rolf O. Karlstrom Joseph Bergan
author_sort	Joseph Dwyer
title	Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force
title_short	Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force
title_full	Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force
title_fullStr	Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force
title_full_unstemmed	Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force
title_sort	accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c1b507e37c8446c3b4426afebbbe9637
work_keys_str_mv	AT josephdwyer acceleratedclearingandmolecularlabelingofbiologicaltissuesusingmagnetohydrodynamicforce AT mdesmondramirez acceleratedclearingandmolecularlabelingofbiologicaltissuesusingmagnetohydrodynamicforce AT paulskatz acceleratedclearingandmolecularlabelingofbiologicaltissuesusingmagnetohydrodynamicforce AT rolfokarlstrom acceleratedclearingandmolecularlabelingofbiologicaltissuesusingmagnetohydrodynamicforce AT josephbergan acceleratedclearingandmolecularlabelingofbiologicaltissuesusingmagnetohydrodynamicforce
_version_	1718377530144063488

Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force

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