The effects of breeding protocol in C57BL/6J mice on adult offspring behaviour.

Animal experiments have demonstrated that a wide range of prenatal exposures can impact on the behaviour of the offspring. However, there is a lack of evidence as to whether the duration of sire exposure could affect such outcomes. We compared two widely used methods for breeding offspring for behav...

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Autores principales:	Claire J Foldi, Darryl W Eyles, John J McGrath, Thomas H J Burne
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c1b51fb30742421cbe17a29fe8f58b1a
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spelling	oai:doaj.org-article:c1b51fb30742421cbe17a29fe8f58b1a2021-11-18T06:56:54ZThe effects of breeding protocol in C57BL/6J mice on adult offspring behaviour.1932-620310.1371/journal.pone.0018152https://doaj.org/article/c1b51fb30742421cbe17a29fe8f58b1a2011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21448436/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Animal experiments have demonstrated that a wide range of prenatal exposures can impact on the behaviour of the offspring. However, there is a lack of evidence as to whether the duration of sire exposure could affect such outcomes. We compared two widely used methods for breeding offspring for behavioural studies. The first involved housing male and female C57Bl/6J mice together for a period of time (usually 10-12 days) and checking for pregnancy by the presence of a distended abdomen (Pair-housed; PH). The second involved daily introduction of female breeders to the male homecage followed by daily checks for pregnancy by the presence of vaginal plugs (Time-mated; TM). Male and female offspring were tested at 10 weeks of age on a behavioural test battery including the elevated plus-maze, hole board, light/dark emergence, forced swim test, novelty-suppressed feeding, active avoidance and extinction, tests for nociception and for prepulse inhibition (PPI) of the acoustic startle response. We found that length of sire exposure (LSE) had no significant effects on offspring behaviour, suggesting that the two breeding protocols do not differentially affect the behavioural outcomes of interest. The absence of LSE effects on the selected variables examined does not detract from the relevance of this study. Information regarding the potential influences of breeding protocol is not only absent from the literature, but also likely to be of particular interest to researchers studying the influence of prenatal manipulations on adult behaviour.Claire J FoldiDarryl W EylesJohn J McGrathThomas H J BurnePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e18152 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claire J Foldi Darryl W Eyles John J McGrath Thomas H J Burne The effects of breeding protocol in C57BL/6J mice on adult offspring behaviour.
description	Animal experiments have demonstrated that a wide range of prenatal exposures can impact on the behaviour of the offspring. However, there is a lack of evidence as to whether the duration of sire exposure could affect such outcomes. We compared two widely used methods for breeding offspring for behavioural studies. The first involved housing male and female C57Bl/6J mice together for a period of time (usually 10-12 days) and checking for pregnancy by the presence of a distended abdomen (Pair-housed; PH). The second involved daily introduction of female breeders to the male homecage followed by daily checks for pregnancy by the presence of vaginal plugs (Time-mated; TM). Male and female offspring were tested at 10 weeks of age on a behavioural test battery including the elevated plus-maze, hole board, light/dark emergence, forced swim test, novelty-suppressed feeding, active avoidance and extinction, tests for nociception and for prepulse inhibition (PPI) of the acoustic startle response. We found that length of sire exposure (LSE) had no significant effects on offspring behaviour, suggesting that the two breeding protocols do not differentially affect the behavioural outcomes of interest. The absence of LSE effects on the selected variables examined does not detract from the relevance of this study. Information regarding the potential influences of breeding protocol is not only absent from the literature, but also likely to be of particular interest to researchers studying the influence of prenatal manipulations on adult behaviour.
format	article
author	Claire J Foldi Darryl W Eyles John J McGrath Thomas H J Burne
author_facet	Claire J Foldi Darryl W Eyles John J McGrath Thomas H J Burne
author_sort	Claire J Foldi
title	The effects of breeding protocol in C57BL/6J mice on adult offspring behaviour.
title_short	The effects of breeding protocol in C57BL/6J mice on adult offspring behaviour.
title_full	The effects of breeding protocol in C57BL/6J mice on adult offspring behaviour.
title_fullStr	The effects of breeding protocol in C57BL/6J mice on adult offspring behaviour.
title_full_unstemmed	The effects of breeding protocol in C57BL/6J mice on adult offspring behaviour.
title_sort	effects of breeding protocol in c57bl/6j mice on adult offspring behaviour.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/c1b51fb30742421cbe17a29fe8f58b1a
work_keys_str_mv	AT clairejfoldi theeffectsofbreedingprotocolinc57bl6jmiceonadultoffspringbehaviour AT darrylweyles theeffectsofbreedingprotocolinc57bl6jmiceonadultoffspringbehaviour AT johnjmcgrath theeffectsofbreedingprotocolinc57bl6jmiceonadultoffspringbehaviour AT thomashjburne theeffectsofbreedingprotocolinc57bl6jmiceonadultoffspringbehaviour AT clairejfoldi effectsofbreedingprotocolinc57bl6jmiceonadultoffspringbehaviour AT darrylweyles effectsofbreedingprotocolinc57bl6jmiceonadultoffspringbehaviour AT johnjmcgrath effectsofbreedingprotocolinc57bl6jmiceonadultoffspringbehaviour AT thomashjburne effectsofbreedingprotocolinc57bl6jmiceonadultoffspringbehaviour
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The effects of breeding protocol in C57BL/6J mice on adult offspring behaviour.

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