Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development

Abstract As one of the best-studied RNA binding proteins in plant, pentatricopeptide repeats (PPRs) protein are mainly targeted to mitochondria and/or chloroplasts for RNA processing to regulate the biogenesis and function of the organelles, but its molecular mechanism and role in development remain...

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Autores principales:	Peng Zhao, Fang Wang, Na Li, Dong-Qiao Shi, Wei-Cai Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c1bdd8238ee34d6f8d2f1fd3f2923c29
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spelling	oai:doaj.org-article:c1bdd8238ee34d6f8d2f1fd3f2923c292021-12-02T14:06:51ZPentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development10.1038/s41598-020-58495-52045-2322https://doaj.org/article/c1bdd8238ee34d6f8d2f1fd3f2923c292020-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-58495-5https://doaj.org/toc/2045-2322Abstract As one of the best-studied RNA binding proteins in plant, pentatricopeptide repeats (PPRs) protein are mainly targeted to mitochondria and/or chloroplasts for RNA processing to regulate the biogenesis and function of the organelles, but its molecular mechanism and role in development remain to be further revealed. Here, we identified a mitochondria-localized P-type small PPR protein, MITOCHONDRION-MEDIATED GROWTH DEFECT 1 (MID1) that is crucial for Arabidopsis development. Mutation in MID1 causes retarded embryo development and stunted plant growth with defects in cell expansion and proliferation. Molecular experiments showed that MID1 is required for the splicing of the nad2 intron 1 in mitochondria. Consistently, mid1 plants display significant reduction in the abundance and activity of mitochondrial respiration complex I, accompanied by abnormal mitochondrial morphology and energy metabolism. Furthermore, MID1 is associated with other trans-factors involved in NICOTINAMIDE ADENINE DINUCLEOTIDE HYDROGEN (NADH) DEHYDROGENASE SUBUNIT 2 (nad2) intron 1 splicing, and interacts directly with itself and MITOCHONDRIAL STABILITY FACTOR 1 (MTSF1). This suggests that MID1 most likely functions as a dimer for nad2 intron 1 splicing. Together, we characterized a novel PPR protein MID1 for nad2 intron 1 splicing.Peng ZhaoFang WangNa LiDong-Qiao ShiWei-Cai YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng Zhao Fang Wang Na Li Dong-Qiao Shi Wei-Cai Yang Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
description	Abstract As one of the best-studied RNA binding proteins in plant, pentatricopeptide repeats (PPRs) protein are mainly targeted to mitochondria and/or chloroplasts for RNA processing to regulate the biogenesis and function of the organelles, but its molecular mechanism and role in development remain to be further revealed. Here, we identified a mitochondria-localized P-type small PPR protein, MITOCHONDRION-MEDIATED GROWTH DEFECT 1 (MID1) that is crucial for Arabidopsis development. Mutation in MID1 causes retarded embryo development and stunted plant growth with defects in cell expansion and proliferation. Molecular experiments showed that MID1 is required for the splicing of the nad2 intron 1 in mitochondria. Consistently, mid1 plants display significant reduction in the abundance and activity of mitochondrial respiration complex I, accompanied by abnormal mitochondrial morphology and energy metabolism. Furthermore, MID1 is associated with other trans-factors involved in NICOTINAMIDE ADENINE DINUCLEOTIDE HYDROGEN (NADH) DEHYDROGENASE SUBUNIT 2 (nad2) intron 1 splicing, and interacts directly with itself and MITOCHONDRIAL STABILITY FACTOR 1 (MTSF1). This suggests that MID1 most likely functions as a dimer for nad2 intron 1 splicing. Together, we characterized a novel PPR protein MID1 for nad2 intron 1 splicing.
format	article
author	Peng Zhao Fang Wang Na Li Dong-Qiao Shi Wei-Cai Yang
author_facet	Peng Zhao Fang Wang Na Li Dong-Qiao Shi Wei-Cai Yang
author_sort	Peng Zhao
title	Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
title_short	Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
title_full	Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
title_fullStr	Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
title_full_unstemmed	Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
title_sort	pentatricopeptide repeat protein mid1 modulates nad2 intron 1 splicing and arabidopsis development
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c1bdd8238ee34d6f8d2f1fd3f2923c29
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Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development

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