Low Cost Strategy for Micropropagation of Lilium Asiatic Hybrid Cv. Toscana

A low cost protocol for in vitro propagation of Lilium cv. Toscana has been developed through incorporation of cost-effective media components. MS medium supplemented with 0.75 mg-1 BAP (6-benzylaminopurine) and 0.5 mg-1 NAA (α-naphthalene-acetic acid) was prepared with tapioca granules, table sugar...

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Autores principales:	R Kaur, Neetu Thakur, D R Sharma
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2006
Materias:	lilium micropropagation low-cost strategy. Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/c1c7f4078230475fb211f6d9431df0ba
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spelling	oai:doaj.org-article:c1c7f4078230475fb211f6d9431df0ba2021-12-02T04:02:09ZLow Cost Strategy for Micropropagation of Lilium Asiatic Hybrid Cv. Toscana0973-354X2582-4899https://doaj.org/article/c1c7f4078230475fb211f6d9431df0ba2006-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/668https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899A low cost protocol for in vitro propagation of Lilium cv. Toscana has been developed through incorporation of cost-effective media components. MS medium supplemented with 0.75 mg-1 BAP (6-benzylaminopurine) and 0.5 mg-1 NAA (α-naphthalene-acetic acid) was prepared with tapioca granules, table sugar and tap water in different combinations in place of agar-agar, sucrose and distilled water, respectively. Culture medium containing all the cost effective components was found to be the best for in vitro establishment of cultures yielding 6.00 bulblets per explant and medium supplemented with tapioca granules as cost effective component was found to be the best for in vitro multiplication of bulblets giving 3.70 bulblets per in vitro formed bulblet five weeks from third subculture. Tapioca supplemented MS medium containing 1 mg-1 NAA was significantly better than all the other modified media giving 86.62% in vitro rooting, 2.86 average root number and 4.60 cm average root length. For hardening of in vitro rooted bulblets, coco peat, peat moss and coco peat in combination with peat moss were found to be at par giving 100% survival.R KaurNeetu ThakurD R SharmaSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticleliliummicropropagationlow-cost strategy.Plant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 1, Iss 1, Pp 24-27 (2006)
institution	DOAJ
collection	DOAJ
language	EN
topic	lilium micropropagation low-cost strategy. Plant culture SB1-1110
spellingShingle	lilium micropropagation low-cost strategy. Plant culture SB1-1110 R Kaur Neetu Thakur D R Sharma Low Cost Strategy for Micropropagation of Lilium Asiatic Hybrid Cv. Toscana
description	A low cost protocol for in vitro propagation of Lilium cv. Toscana has been developed through incorporation of cost-effective media components. MS medium supplemented with 0.75 mg-1 BAP (6-benzylaminopurine) and 0.5 mg-1 NAA (α-naphthalene-acetic acid) was prepared with tapioca granules, table sugar and tap water in different combinations in place of agar-agar, sucrose and distilled water, respectively. Culture medium containing all the cost effective components was found to be the best for in vitro establishment of cultures yielding 6.00 bulblets per explant and medium supplemented with tapioca granules as cost effective component was found to be the best for in vitro multiplication of bulblets giving 3.70 bulblets per in vitro formed bulblet five weeks from third subculture. Tapioca supplemented MS medium containing 1 mg-1 NAA was significantly better than all the other modified media giving 86.62% in vitro rooting, 2.86 average root number and 4.60 cm average root length. For hardening of in vitro rooted bulblets, coco peat, peat moss and coco peat in combination with peat moss were found to be at par giving 100% survival.
format	article
author	R Kaur Neetu Thakur D R Sharma
author_facet	R Kaur Neetu Thakur D R Sharma
author_sort	R Kaur
title	Low Cost Strategy for Micropropagation of Lilium Asiatic Hybrid Cv. Toscana
title_short	Low Cost Strategy for Micropropagation of Lilium Asiatic Hybrid Cv. Toscana
title_full	Low Cost Strategy for Micropropagation of Lilium Asiatic Hybrid Cv. Toscana
title_fullStr	Low Cost Strategy for Micropropagation of Lilium Asiatic Hybrid Cv. Toscana
title_full_unstemmed	Low Cost Strategy for Micropropagation of Lilium Asiatic Hybrid Cv. Toscana
title_sort	low cost strategy for micropropagation of lilium asiatic hybrid cv. toscana
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2006
url	https://doaj.org/article/c1c7f4078230475fb211f6d9431df0ba
work_keys_str_mv	AT rkaur lowcoststrategyformicropropagationofliliumasiatichybridcvtoscana AT neetuthakur lowcoststrategyformicropropagationofliliumasiatichybridcvtoscana AT drsharma lowcoststrategyformicropropagationofliliumasiatichybridcvtoscana
_version_	1718401514381246464

Low Cost Strategy for Micropropagation of Lilium Asiatic Hybrid Cv. Toscana

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