SUBPOPULATION COMPOSITION OF PERIPHERAL BLOOD LYMPHOCYTES IN UVEAL MELANOMA

Uveal melanoma is a malignant neuroectodermal tumor. Despite of radical primary treatment with the use of enucleation of the eye or radiation therapy, almost in 50% of patients develop metastatic disease. The effectiveness of various methods of treatment of malignant tumors, the prognosis of the dis...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Svetlana Vladimirovna Saakyan, Natalya Vladimirovna Balatskaya, Ludmila Anatolievna Katargina, Irina Gennadievna Kulikova, Elena Borisovna Myakoshina
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	subpopulation of peripheral blood lymphocytes uveal melanoma Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/c1c8100bf35f469389e77eeeca354e33
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c1c8100bf35f469389e77eeeca354e33
record_format	dspace
spelling	oai:doaj.org-article:c1c8100bf35f469389e77eeeca354e332021-11-18T08:03:48ZSUBPOPULATION COMPOSITION OF PERIPHERAL BLOOD LYMPHOCYTES IN UVEAL MELANOMA1563-06252313-741X10.15789/1563-0625-2019-4-765-772https://doaj.org/article/c1c8100bf35f469389e77eeeca354e332019-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1677https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XUveal melanoma is a malignant neuroectodermal tumor. Despite of radical primary treatment with the use of enucleation of the eye or radiation therapy, almost in 50% of patients develop metastatic disease. The effectiveness of various methods of treatment of malignant tumors, the prognosis of the disease largely depend on the state of the patient's immune system. Data on the ratio of lymphocytes of different populations, their relationship with the stages of uveal melanoma are currently not available. 84 patients with uveal melanoma were examined (44 women and 40 men; mean age 53.7 ± 12.2 years). In group 1 (small tumors) included 36 patients, in the 2nd (medium-sized tumors) - 26 patients, 22 patients made up the 3rd group (large tumors). The control group included 33 practically healthy donors. The material of the study was samples of whole blood taken from the ulnar vein on an empty stomach in the morning with the help of vacuum systems in test tubes Vacuette® with anticoagulant K3EDTA. Immunophenotyping was performed using flow laser cytofluorimetry using the Multitest 6-Color TBNK Reagent monoclonal antibody system in BD TruСount test tubes (Becton Dickinson, USA), BD FACSCanto II cytometer (Becton Dickinson, USA). Erythrocyte lysis and leukocyte fixation were performed using a BD FACS ™ Lysing Solution lysing solution (Becton Dickinson, USA). The relative and absolute levels of lymphocyte populations and subpopulations were determined in the Canto program (Becton Dickinson, USA), with the selection of the analyzed region in the general population expressing CD45 + antigen and cell granularity (CD45 + PerCP-Cy5,5 * / SSC); fluorochromes labeled antibodies to CD3 + (FITC), CD4 + (PE-Cy7 ), CD8 + (APC-CY7 ), CD16 + / 56 + (PE), CD19 + (APC *) were used to differentiate: T-lymphocytes (CD3 +), T-helper cells (CD3 + CD4 + CD8-), T-cytotoxic (CD3 + CD4-CD8 +), T-double positive (CD3 + CD4 + CD8 +), NK-cells (CD16 + CD56 +), B-lymphocytes (CD19 +) , calculate the ratio of CD3 + CD4 + / CD3 + CD8 + subpopulations - the index reflecting the balance of T-helper cells and cytotoxic T-cells (CD4 + / CD8 +). Our individual analysis of the composition of lymphocytes allows us to conclude that the growth of uveal melanoma is accompanied by systemic multidirectional changes in the qualitative and quantitative composition of immunocompetent cells, affecting both innate (NK cells) and adaptive (T-lymphocytes) links of antitumor immune defense. The results are important for the development of personalized approaches to the prognosis and treatment of patients with uveal melanoma. The dynamics of changes in the quantitative composition of lymphocyte subpopulations in oncological diseases may be of value for monitoring immunotherapeutic effects and supplement the clinical assessment of the course of the underlying disease.Svetlana Vladimirovna SaakyanNatalya Vladimirovna BalatskayaLudmila Anatolievna KatarginaIrina Gennadievna KulikovaElena Borisovna MyakoshinaSPb RAACIarticlesubpopulation of peripheral blood lymphocytesuveal melanomaImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 4, Pp 765-772 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	subpopulation of peripheral blood lymphocytes uveal melanoma Immunologic diseases. Allergy RC581-607
spellingShingle	subpopulation of peripheral blood lymphocytes uveal melanoma Immunologic diseases. Allergy RC581-607 Svetlana Vladimirovna Saakyan Natalya Vladimirovna Balatskaya Ludmila Anatolievna Katargina Irina Gennadievna Kulikova Elena Borisovna Myakoshina SUBPOPULATION COMPOSITION OF PERIPHERAL BLOOD LYMPHOCYTES IN UVEAL MELANOMA
description	Uveal melanoma is a malignant neuroectodermal tumor. Despite of radical primary treatment with the use of enucleation of the eye or radiation therapy, almost in 50% of patients develop metastatic disease. The effectiveness of various methods of treatment of malignant tumors, the prognosis of the disease largely depend on the state of the patient's immune system. Data on the ratio of lymphocytes of different populations, their relationship with the stages of uveal melanoma are currently not available. 84 patients with uveal melanoma were examined (44 women and 40 men; mean age 53.7 ± 12.2 years). In group 1 (small tumors) included 36 patients, in the 2nd (medium-sized tumors) - 26 patients, 22 patients made up the 3rd group (large tumors). The control group included 33 practically healthy donors. The material of the study was samples of whole blood taken from the ulnar vein on an empty stomach in the morning with the help of vacuum systems in test tubes Vacuette® with anticoagulant K3EDTA. Immunophenotyping was performed using flow laser cytofluorimetry using the Multitest 6-Color TBNK Reagent monoclonal antibody system in BD TruСount test tubes (Becton Dickinson, USA), BD FACSCanto II cytometer (Becton Dickinson, USA). Erythrocyte lysis and leukocyte fixation were performed using a BD FACS ™ Lysing Solution lysing solution (Becton Dickinson, USA). The relative and absolute levels of lymphocyte populations and subpopulations were determined in the Canto program (Becton Dickinson, USA), with the selection of the analyzed region in the general population expressing CD45 + antigen and cell granularity (CD45 + PerCP-Cy5,5 * / SSC); fluorochromes labeled antibodies to CD3 + (FITC), CD4 + (PE-Cy7 ), CD8 + (APC-CY7 ), CD16 + / 56 + (PE), CD19 + (APC *) were used to differentiate: T-lymphocytes (CD3 +), T-helper cells (CD3 + CD4 + CD8-), T-cytotoxic (CD3 + CD4-CD8 +), T-double positive (CD3 + CD4 + CD8 +), NK-cells (CD16 + CD56 +), B-lymphocytes (CD19 +) , calculate the ratio of CD3 + CD4 + / CD3 + CD8 + subpopulations - the index reflecting the balance of T-helper cells and cytotoxic T-cells (CD4 + / CD8 +). Our individual analysis of the composition of lymphocytes allows us to conclude that the growth of uveal melanoma is accompanied by systemic multidirectional changes in the qualitative and quantitative composition of immunocompetent cells, affecting both innate (NK cells) and adaptive (T-lymphocytes) links of antitumor immune defense. The results are important for the development of personalized approaches to the prognosis and treatment of patients with uveal melanoma. The dynamics of changes in the quantitative composition of lymphocyte subpopulations in oncological diseases may be of value for monitoring immunotherapeutic effects and supplement the clinical assessment of the course of the underlying disease.
format	article
author	Svetlana Vladimirovna Saakyan Natalya Vladimirovna Balatskaya Ludmila Anatolievna Katargina Irina Gennadievna Kulikova Elena Borisovna Myakoshina
author_facet	Svetlana Vladimirovna Saakyan Natalya Vladimirovna Balatskaya Ludmila Anatolievna Katargina Irina Gennadievna Kulikova Elena Borisovna Myakoshina
author_sort	Svetlana Vladimirovna Saakyan
title	SUBPOPULATION COMPOSITION OF PERIPHERAL BLOOD LYMPHOCYTES IN UVEAL MELANOMA
title_short	SUBPOPULATION COMPOSITION OF PERIPHERAL BLOOD LYMPHOCYTES IN UVEAL MELANOMA
title_full	SUBPOPULATION COMPOSITION OF PERIPHERAL BLOOD LYMPHOCYTES IN UVEAL MELANOMA
title_fullStr	SUBPOPULATION COMPOSITION OF PERIPHERAL BLOOD LYMPHOCYTES IN UVEAL MELANOMA
title_full_unstemmed	SUBPOPULATION COMPOSITION OF PERIPHERAL BLOOD LYMPHOCYTES IN UVEAL MELANOMA
title_sort	subpopulation composition of peripheral blood lymphocytes in uveal melanoma
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/c1c8100bf35f469389e77eeeca354e33
work_keys_str_mv	AT svetlanavladimirovnasaakyan subpopulationcompositionofperipheralbloodlymphocytesinuvealmelanoma AT natalyavladimirovnabalatskaya subpopulationcompositionofperipheralbloodlymphocytesinuvealmelanoma AT ludmilaanatolievnakatargina subpopulationcompositionofperipheralbloodlymphocytesinuvealmelanoma AT irinagennadievnakulikova subpopulationcompositionofperipheralbloodlymphocytesinuvealmelanoma AT elenaborisovnamyakoshina subpopulationcompositionofperipheralbloodlymphocytesinuvealmelanoma
_version_	1718422378210394112

SUBPOPULATION COMPOSITION OF PERIPHERAL BLOOD LYMPHOCYTES IN UVEAL MELANOMA

Ejemplares similares