Transurethral versus suprapubic catheterization to test urethral function in rats

Abstract Transurethral and suprapubic catheterization have both been used to test urethral function in rats; however, it is unknown whether these methods affect urethral function or if the order of catheterization affects the results. The aim of this cross-over designed experiment was to compare the...

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Autores principales:	Kristine Janssen, Kangli Deng, Steve J. A. Majerus, Dan Li Lin, Brett Hanzlicek, Robert S. Butler, Carl H. van der Vaart, Margot S. Damaser
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c1c8cf71a4a14ee4a5db39478fb8dac2
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spelling	oai:doaj.org-article:c1c8cf71a4a14ee4a5db39478fb8dac22021-12-02T18:30:51ZTransurethral versus suprapubic catheterization to test urethral function in rats10.1038/s41598-021-93772-x2045-2322https://doaj.org/article/c1c8cf71a4a14ee4a5db39478fb8dac22021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93772-xhttps://doaj.org/toc/2045-2322Abstract Transurethral and suprapubic catheterization have both been used to test urethral function in rats; however, it is unknown whether these methods affect urethral function or if the order of catheterization affects the results. The aim of this cross-over designed experiment was to compare the effects of catheterization methods and order on leak point pressure (LPP) testing. LPP and simultaneous external urethral sphincter electromyography (EUS EMG) were recorded in anesthetized female virgin Sprague-Dawley rats in a cross-over design to test the effects of transurethral and suprapubic catheterization. There was no significant difference in peak bladder pressure during LPP testing whether measured with a transurethral or suprapubic catheter. There was no significant difference in peak bladder pressure between the first and second catheter insertions. However, peak EMG firing rate, as well as peak EMG amplitude and EMG amplitude difference between peak and baseline were significantly higher after the first catheter insertion compared to the second insertion, regardless of the catheter method. Our results suggest that route of catheterization does not alter urethral function, e.g. create a functional partial outlet obstruction. Either catheterization method could be used for LPP and/or EUS EMG testing in rats.Kristine JanssenKangli DengSteve J. A. MajerusDan Li LinBrett HanzlicekRobert S. ButlerCarl H. van der VaartMargot S. DamaserNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristine Janssen Kangli Deng Steve J. A. Majerus Dan Li Lin Brett Hanzlicek Robert S. Butler Carl H. van der Vaart Margot S. Damaser Transurethral versus suprapubic catheterization to test urethral function in rats
description	Abstract Transurethral and suprapubic catheterization have both been used to test urethral function in rats; however, it is unknown whether these methods affect urethral function or if the order of catheterization affects the results. The aim of this cross-over designed experiment was to compare the effects of catheterization methods and order on leak point pressure (LPP) testing. LPP and simultaneous external urethral sphincter electromyography (EUS EMG) were recorded in anesthetized female virgin Sprague-Dawley rats in a cross-over design to test the effects of transurethral and suprapubic catheterization. There was no significant difference in peak bladder pressure during LPP testing whether measured with a transurethral or suprapubic catheter. There was no significant difference in peak bladder pressure between the first and second catheter insertions. However, peak EMG firing rate, as well as peak EMG amplitude and EMG amplitude difference between peak and baseline were significantly higher after the first catheter insertion compared to the second insertion, regardless of the catheter method. Our results suggest that route of catheterization does not alter urethral function, e.g. create a functional partial outlet obstruction. Either catheterization method could be used for LPP and/or EUS EMG testing in rats.
format	article
author	Kristine Janssen Kangli Deng Steve J. A. Majerus Dan Li Lin Brett Hanzlicek Robert S. Butler Carl H. van der Vaart Margot S. Damaser
author_facet	Kristine Janssen Kangli Deng Steve J. A. Majerus Dan Li Lin Brett Hanzlicek Robert S. Butler Carl H. van der Vaart Margot S. Damaser
author_sort	Kristine Janssen
title	Transurethral versus suprapubic catheterization to test urethral function in rats
title_short	Transurethral versus suprapubic catheterization to test urethral function in rats
title_full	Transurethral versus suprapubic catheterization to test urethral function in rats
title_fullStr	Transurethral versus suprapubic catheterization to test urethral function in rats
title_full_unstemmed	Transurethral versus suprapubic catheterization to test urethral function in rats
title_sort	transurethral versus suprapubic catheterization to test urethral function in rats
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c1c8cf71a4a14ee4a5db39478fb8dac2
work_keys_str_mv	AT kristinejanssen transurethralversussuprapubiccatheterizationtotesturethralfunctioninrats AT kanglideng transurethralversussuprapubiccatheterizationtotesturethralfunctioninrats AT stevejamajerus transurethralversussuprapubiccatheterizationtotesturethralfunctioninrats AT danlilin transurethralversussuprapubiccatheterizationtotesturethralfunctioninrats AT bretthanzlicek transurethralversussuprapubiccatheterizationtotesturethralfunctioninrats AT robertsbutler transurethralversussuprapubiccatheterizationtotesturethralfunctioninrats AT carlhvandervaart transurethralversussuprapubiccatheterizationtotesturethralfunctioninrats AT margotsdamaser transurethralversussuprapubiccatheterizationtotesturethralfunctioninrats
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Transurethral versus suprapubic catheterization to test urethral function in rats

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