Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice

Abstract Sheath blight, caused by the necrotrophic fungal pathogen Rhizoctonia solani, is a serious and destructive disease of the rice. In order to improve sheath blight resistance, we developed three different kinds of transgenic rice lines. The first transgenic line overexpresses the rice chitina...

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Autores principales:	Subhasis Karmakar, Kutubuddin A. Molla, Kaushik Das, Sailendra Nath Sarkar, Swapan K. Datta, Karabi Datta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c1c962dd28e4422eb9023e227c23f537
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spelling	oai:doaj.org-article:c1c962dd28e4422eb9023e227c23f5372021-12-02T11:52:57ZDual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice10.1038/s41598-017-08180-x2045-2322https://doaj.org/article/c1c962dd28e4422eb9023e227c23f5372017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08180-xhttps://doaj.org/toc/2045-2322Abstract Sheath blight, caused by the necrotrophic fungal pathogen Rhizoctonia solani, is a serious and destructive disease of the rice. In order to improve sheath blight resistance, we developed three different kinds of transgenic rice lines. The first transgenic line overexpresses the rice chitinase gene (OsCHI11); the second contains the Arabidopsis NPR1 (AtNPR1) gene and, the third has pyramided constructs with both the genes (OsCHI11 and AtNPR1). This is a comparative study between the single-gene transgenic lines and the double gene transgenic in terms of their ability to activate the plant defense system. Rice plants of each individual construct were screened via PCR, Southern hybridization, activity assays, and expression analysis. The best transgenic lines of each construct were chosen for comparative study. The fold change in qRT-PCR and activity assays revealed that the pyramided transgenic rice plants show a significant upregulation of defense-related genes, PR genes, and antioxidant marker genes as compared to the single transgene. Simultaneous co-expression of both the genes was found to be more efficient in tolerating oxidative stress. In R. solani (RS) toxin assay, mycelial agar disc bioassay, and in vivo plant bioassay, pyramided transgenic plant lines were more competent at restricting the pathogen development and enhancing sheath blight tolerance as compared to single gene transformants.Subhasis KarmakarKutubuddin A. MollaKaushik DasSailendra Nath SarkarSwapan K. DattaKarabi DattaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Subhasis Karmakar Kutubuddin A. Molla Kaushik Das Sailendra Nath Sarkar Swapan K. Datta Karabi Datta Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice
description	Abstract Sheath blight, caused by the necrotrophic fungal pathogen Rhizoctonia solani, is a serious and destructive disease of the rice. In order to improve sheath blight resistance, we developed three different kinds of transgenic rice lines. The first transgenic line overexpresses the rice chitinase gene (OsCHI11); the second contains the Arabidopsis NPR1 (AtNPR1) gene and, the third has pyramided constructs with both the genes (OsCHI11 and AtNPR1). This is a comparative study between the single-gene transgenic lines and the double gene transgenic in terms of their ability to activate the plant defense system. Rice plants of each individual construct were screened via PCR, Southern hybridization, activity assays, and expression analysis. The best transgenic lines of each construct were chosen for comparative study. The fold change in qRT-PCR and activity assays revealed that the pyramided transgenic rice plants show a significant upregulation of defense-related genes, PR genes, and antioxidant marker genes as compared to the single transgene. Simultaneous co-expression of both the genes was found to be more efficient in tolerating oxidative stress. In R. solani (RS) toxin assay, mycelial agar disc bioassay, and in vivo plant bioassay, pyramided transgenic plant lines were more competent at restricting the pathogen development and enhancing sheath blight tolerance as compared to single gene transformants.
format	article
author	Subhasis Karmakar Kutubuddin A. Molla Kaushik Das Sailendra Nath Sarkar Swapan K. Datta Karabi Datta
author_facet	Subhasis Karmakar Kutubuddin A. Molla Kaushik Das Sailendra Nath Sarkar Swapan K. Datta Karabi Datta
author_sort	Subhasis Karmakar
title	Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice
title_short	Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice
title_full	Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice
title_fullStr	Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice
title_full_unstemmed	Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice
title_sort	dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c1c962dd28e4422eb9023e227c23f537
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Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice

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