Optimized extraction of polyphenols from leaves of Rosemary (Rosmarinus officinalis L.) grown in Lam Dong province, Vietnam, and evaluation of their antioxidant capacity

In the present study, the optimized solvent extraction conditions with regards to the total polyphenol content (TPC) and antioxidant capacity of rosemary leaf extract (RLE) were determined. The one-factor-at-a-time method was used to independently investigate the effect of several extraction paramet...

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Autores principales:	Nguyen-Kim Minh-Tam, Truong Quoc-Cuong, Nguyen Minh-Thuy, Cao-Thi Bich-Hang, Tong Thanh-Danh, Dao Tan Phat, Tran Thien Hien, Van Tan Lam, Le Xuan-Tien
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	maceration extraction rosmarinus officinalis optimization polyphenol content antioxidant activity Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/c1cacd9a081745a2bd3c841d8a44b468
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spelling	oai:doaj.org-article:c1cacd9a081745a2bd3c841d8a44b4682021-12-05T14:10:44ZOptimized extraction of polyphenols from leaves of Rosemary (Rosmarinus officinalis L.) grown in Lam Dong province, Vietnam, and evaluation of their antioxidant capacity2391-542010.1515/chem-2021-0061https://doaj.org/article/c1cacd9a081745a2bd3c841d8a44b4682021-10-01T00:00:00Zhttps://doi.org/10.1515/chem-2021-0061https://doaj.org/toc/2391-5420In the present study, the optimized solvent extraction conditions with regards to the total polyphenol content (TPC) and antioxidant capacity of rosemary leaf extract (RLE) were determined. The one-factor-at-a-time method was used to independently investigate the effect of several extraction parameters, including ethanol concentration (0–100% v/v), extraction temperature (50–80°C), extraction period (15–60 min), material–solvent ratio (1:5–1:10 g/mL), and extraction cycles (1, 2, and 3 times) on polyphenol content. Response surface methodology (RSM), in combination with a central composite design, was used to perform optimization. The following optimal conditions that gave maximal TPC were determined and experimentally verified: ethanol concentration of 65% (v/v), extraction temperature of 65°C, material–solvent ratio of 1:7.5 g/mL, extraction time of 15 min, and 2 cycles of extraction. These parameters corresponded with the TPC yield of 87.42 ± 0.25 mg gallic acid equivalent/g dried feed material (mg GAE/g DW). The optimal conditions gave a high extraction yield (337 ± 6 mg dried extract/g dried feed material) with 197.28 ± 3.11 mg GAE/g dried extract. The estimated models were strongly significant (p < 0.05) for TPC values with significant regression coefficients (R 2) of 0.9979. The obtained RLE was supposed to be the top grade of natural antioxidant with the IC50 (DPPH assays) value of 9.4 ± 0.1 μg/mL, which is higher than that of the vitamin C by just three times (IC50 = 3.2 ± 0.1 μg/mL). Current results justify RLE as a potential agent in food preservation applications.Nguyen-Kim Minh-TamTruong Quoc-CuongNguyen Minh-ThuyCao-Thi Bich-HangTong Thanh-DanhDao Tan PhatTran Thien HienVan Tan LamLe Xuan-TienDe Gruyterarticlemaceration extractionrosmarinus officinalisoptimizationpolyphenol contentantioxidant activityChemistryQD1-999ENOpen Chemistry, Vol 19, Iss 1, Pp 1043-1051 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	maceration extraction rosmarinus officinalis optimization polyphenol content antioxidant activity Chemistry QD1-999
spellingShingle	maceration extraction rosmarinus officinalis optimization polyphenol content antioxidant activity Chemistry QD1-999 Nguyen-Kim Minh-Tam Truong Quoc-Cuong Nguyen Minh-Thuy Cao-Thi Bich-Hang Tong Thanh-Danh Dao Tan Phat Tran Thien Hien Van Tan Lam Le Xuan-Tien Optimized extraction of polyphenols from leaves of Rosemary (Rosmarinus officinalis L.) grown in Lam Dong province, Vietnam, and evaluation of their antioxidant capacity
description	In the present study, the optimized solvent extraction conditions with regards to the total polyphenol content (TPC) and antioxidant capacity of rosemary leaf extract (RLE) were determined. The one-factor-at-a-time method was used to independently investigate the effect of several extraction parameters, including ethanol concentration (0–100% v/v), extraction temperature (50–80°C), extraction period (15–60 min), material–solvent ratio (1:5–1:10 g/mL), and extraction cycles (1, 2, and 3 times) on polyphenol content. Response surface methodology (RSM), in combination with a central composite design, was used to perform optimization. The following optimal conditions that gave maximal TPC were determined and experimentally verified: ethanol concentration of 65% (v/v), extraction temperature of 65°C, material–solvent ratio of 1:7.5 g/mL, extraction time of 15 min, and 2 cycles of extraction. These parameters corresponded with the TPC yield of 87.42 ± 0.25 mg gallic acid equivalent/g dried feed material (mg GAE/g DW). The optimal conditions gave a high extraction yield (337 ± 6 mg dried extract/g dried feed material) with 197.28 ± 3.11 mg GAE/g dried extract. The estimated models were strongly significant (p < 0.05) for TPC values with significant regression coefficients (R 2) of 0.9979. The obtained RLE was supposed to be the top grade of natural antioxidant with the IC50 (DPPH assays) value of 9.4 ± 0.1 μg/mL, which is higher than that of the vitamin C by just three times (IC50 = 3.2 ± 0.1 μg/mL). Current results justify RLE as a potential agent in food preservation applications.
format	article
author	Nguyen-Kim Minh-Tam Truong Quoc-Cuong Nguyen Minh-Thuy Cao-Thi Bich-Hang Tong Thanh-Danh Dao Tan Phat Tran Thien Hien Van Tan Lam Le Xuan-Tien
author_facet	Nguyen-Kim Minh-Tam Truong Quoc-Cuong Nguyen Minh-Thuy Cao-Thi Bich-Hang Tong Thanh-Danh Dao Tan Phat Tran Thien Hien Van Tan Lam Le Xuan-Tien
author_sort	Nguyen-Kim Minh-Tam
title	Optimized extraction of polyphenols from leaves of Rosemary (Rosmarinus officinalis L.) grown in Lam Dong province, Vietnam, and evaluation of their antioxidant capacity
title_short	Optimized extraction of polyphenols from leaves of Rosemary (Rosmarinus officinalis L.) grown in Lam Dong province, Vietnam, and evaluation of their antioxidant capacity
title_full	Optimized extraction of polyphenols from leaves of Rosemary (Rosmarinus officinalis L.) grown in Lam Dong province, Vietnam, and evaluation of their antioxidant capacity
title_fullStr	Optimized extraction of polyphenols from leaves of Rosemary (Rosmarinus officinalis L.) grown in Lam Dong province, Vietnam, and evaluation of their antioxidant capacity
title_full_unstemmed	Optimized extraction of polyphenols from leaves of Rosemary (Rosmarinus officinalis L.) grown in Lam Dong province, Vietnam, and evaluation of their antioxidant capacity
title_sort	optimized extraction of polyphenols from leaves of rosemary (rosmarinus officinalis l.) grown in lam dong province, vietnam, and evaluation of their antioxidant capacity
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/c1cacd9a081745a2bd3c841d8a44b468
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Optimized extraction of polyphenols from leaves of Rosemary (Rosmarinus officinalis L.) grown in Lam Dong province, Vietnam, and evaluation of their antioxidant capacity

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