Long non-coding RNA LINC00958 promotes colorectal cancer progression by enhancing the expression of LEM domain containing 1 via microRNA miR-3064-5p

Colorectal cancer is a common cause of cancer-related death worldwide. Thus, there is an urgent need to determine the mechanism of progression of colorectal cancer. In this study, we investigated the function and mechanism of long non-coding RNA LINC00958, providing a new biomarker for colorectal ca...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhaoxia Luo, Shunxin Hao, Jian Yuan, Kai Zhu, Shuo Liu, Jing Zhang, Lei Yao
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	linc00958 mir-3064-5p lemd1 colorectal cancer apoptosis migration invasion Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/c1d47e2fcc0841d69cb162001d2c44d3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c1d47e2fcc0841d69cb162001d2c44d3
record_format	dspace
spelling	oai:doaj.org-article:c1d47e2fcc0841d69cb162001d2c44d32021-11-04T15:51:53ZLong non-coding RNA LINC00958 promotes colorectal cancer progression by enhancing the expression of LEM domain containing 1 via microRNA miR-3064-5p2165-59792165-598710.1080/21655979.2021.1985259https://doaj.org/article/c1d47e2fcc0841d69cb162001d2c44d32021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1985259https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Colorectal cancer is a common cause of cancer-related death worldwide. Thus, there is an urgent need to determine the mechanism of progression of colorectal cancer. In this study, we investigated the function and mechanism of long non-coding RNA LINC00958, providing a new biomarker for colorectal cancer. The expression of LINC00958, miR-3064-5p, and LEM domain containing 1 (LEMD1) in colorectal cancer tissues and cell lines was analyzed using reverse transcription quantitative polymerase chain reaction (RT-qPCR). The interaction between LINC00958, miR-3064-5p, and LEMD1 was assessed using the luciferase assay. The viability, proliferation, migration, invasion, and apoptosis of colorectal cancer cells with silenced LINC00958, miR-3064-5p, and LEMD1 were investigated using the cell counting kit-8 (CCK-8), 5′-Bromo-2′-deoxyuridine (BrdU), flow cytometry, wound healing, and transwell assays. Phosphorylated phosphoinositide 3-kinase (p-PI3K) and phosphorylated protein kinase B (p-AKT) protein levels were measured by western blotting. LINC00958 and LEMD1 were found to have increased, while the expression of miR-3064-5p was decreased in colorectal cancer tissues and cell lines. Silencing of LINC00958 hampered cell viability, proliferation, migration, and invasion, while enhancing the apoptosis in colorectal cancer cells. Notably, LINC00958 inhibited miR-3064-5p and promoted LEMD1; the miR-3064-5p inhibitor abrogated the effect of LINC00958 silencing in colorectal cancer cells. Additionally, LEMD1 knockdown inhibited the activation of PI3K/AKT signaling. Our analyses have shown that LINC00958 could facilitate the progression of colorectal cancer by sponging miR-3064-5p and releasing LEMD1, leading to the activation of the PI3K/AKT pathway. Thus, LINC00958 may be considered as an effective biomarker for the treatment of colorectal cancer.Zhaoxia LuoShunxin HaoJian YuanKai ZhuShuo LiuJing ZhangLei YaoTaylor & Francis Grouparticlelinc00958mir-3064-5plemd1colorectal cancerapoptosismigrationinvasionBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8100-8115 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	linc00958 mir-3064-5p lemd1 colorectal cancer apoptosis migration invasion Biotechnology TP248.13-248.65
spellingShingle	linc00958 mir-3064-5p lemd1 colorectal cancer apoptosis migration invasion Biotechnology TP248.13-248.65 Zhaoxia Luo Shunxin Hao Jian Yuan Kai Zhu Shuo Liu Jing Zhang Lei Yao Long non-coding RNA LINC00958 promotes colorectal cancer progression by enhancing the expression of LEM domain containing 1 via microRNA miR-3064-5p
description	Colorectal cancer is a common cause of cancer-related death worldwide. Thus, there is an urgent need to determine the mechanism of progression of colorectal cancer. In this study, we investigated the function and mechanism of long non-coding RNA LINC00958, providing a new biomarker for colorectal cancer. The expression of LINC00958, miR-3064-5p, and LEM domain containing 1 (LEMD1) in colorectal cancer tissues and cell lines was analyzed using reverse transcription quantitative polymerase chain reaction (RT-qPCR). The interaction between LINC00958, miR-3064-5p, and LEMD1 was assessed using the luciferase assay. The viability, proliferation, migration, invasion, and apoptosis of colorectal cancer cells with silenced LINC00958, miR-3064-5p, and LEMD1 were investigated using the cell counting kit-8 (CCK-8), 5′-Bromo-2′-deoxyuridine (BrdU), flow cytometry, wound healing, and transwell assays. Phosphorylated phosphoinositide 3-kinase (p-PI3K) and phosphorylated protein kinase B (p-AKT) protein levels were measured by western blotting. LINC00958 and LEMD1 were found to have increased, while the expression of miR-3064-5p was decreased in colorectal cancer tissues and cell lines. Silencing of LINC00958 hampered cell viability, proliferation, migration, and invasion, while enhancing the apoptosis in colorectal cancer cells. Notably, LINC00958 inhibited miR-3064-5p and promoted LEMD1; the miR-3064-5p inhibitor abrogated the effect of LINC00958 silencing in colorectal cancer cells. Additionally, LEMD1 knockdown inhibited the activation of PI3K/AKT signaling. Our analyses have shown that LINC00958 could facilitate the progression of colorectal cancer by sponging miR-3064-5p and releasing LEMD1, leading to the activation of the PI3K/AKT pathway. Thus, LINC00958 may be considered as an effective biomarker for the treatment of colorectal cancer.
format	article
author	Zhaoxia Luo Shunxin Hao Jian Yuan Kai Zhu Shuo Liu Jing Zhang Lei Yao
author_facet	Zhaoxia Luo Shunxin Hao Jian Yuan Kai Zhu Shuo Liu Jing Zhang Lei Yao
author_sort	Zhaoxia Luo
title	Long non-coding RNA LINC00958 promotes colorectal cancer progression by enhancing the expression of LEM domain containing 1 via microRNA miR-3064-5p
title_short	Long non-coding RNA LINC00958 promotes colorectal cancer progression by enhancing the expression of LEM domain containing 1 via microRNA miR-3064-5p
title_full	Long non-coding RNA LINC00958 promotes colorectal cancer progression by enhancing the expression of LEM domain containing 1 via microRNA miR-3064-5p
title_fullStr	Long non-coding RNA LINC00958 promotes colorectal cancer progression by enhancing the expression of LEM domain containing 1 via microRNA miR-3064-5p
title_full_unstemmed	Long non-coding RNA LINC00958 promotes colorectal cancer progression by enhancing the expression of LEM domain containing 1 via microRNA miR-3064-5p
title_sort	long non-coding rna linc00958 promotes colorectal cancer progression by enhancing the expression of lem domain containing 1 via microrna mir-3064-5p
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/c1d47e2fcc0841d69cb162001d2c44d3
work_keys_str_mv	AT zhaoxialuo longnoncodingrnalinc00958promotescolorectalcancerprogressionbyenhancingtheexpressionoflemdomaincontaining1viamicrornamir30645p AT shunxinhao longnoncodingrnalinc00958promotescolorectalcancerprogressionbyenhancingtheexpressionoflemdomaincontaining1viamicrornamir30645p AT jianyuan longnoncodingrnalinc00958promotescolorectalcancerprogressionbyenhancingtheexpressionoflemdomaincontaining1viamicrornamir30645p AT kaizhu longnoncodingrnalinc00958promotescolorectalcancerprogressionbyenhancingtheexpressionoflemdomaincontaining1viamicrornamir30645p AT shuoliu longnoncodingrnalinc00958promotescolorectalcancerprogressionbyenhancingtheexpressionoflemdomaincontaining1viamicrornamir30645p AT jingzhang longnoncodingrnalinc00958promotescolorectalcancerprogressionbyenhancingtheexpressionoflemdomaincontaining1viamicrornamir30645p AT leiyao longnoncodingrnalinc00958promotescolorectalcancerprogressionbyenhancingtheexpressionoflemdomaincontaining1viamicrornamir30645p
_version_	1718444738331279360

Long non-coding RNA LINC00958 promotes colorectal cancer progression by enhancing the expression of LEM domain containing 1 via microRNA miR-3064-5p

Ejemplares similares