Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling

Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling

Tubulin post-translational modifications (PTMs) occur spatiotemporally throughout cells, therefore assessing the physiological roles in specific subcellular compartments has been challenging. Here the authors develop a method to rapidly deplete tubulin glutamylation inside the primary cilia by targe...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Shi-Rong Hong, Cuei-Ling Wang, Yao-Shen Huang, Yu-Chen Chang, Ya-Chu Chang, Ganesh V. Pusapati, Chun-Yu Lin, Ning Hsu, Hsiao-Chi Cheng, Yueh-Chen Chiang, Wei-En Huang, Nathan C. Shaner, Rajat Rohatgi, Takanari Inoue, Yu-Chun Lin
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
Materias:
Science
Q
Acceso en línea:https://doaj.org/article/c1e3bf213f4f48708f15f622d8dd14fd
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:c1e3bf213f4f48708f15f622d8dd14fd
record_format dspace
spelling oai:doaj.org-article:c1e3bf213f4f48708f15f622d8dd14fd2021-12-02T17:31:43ZSpatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling10.1038/s41467-018-03952-z2041-1723https://doaj.org/article/c1e3bf213f4f48708f15f622d8dd14fd2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03952-zhttps://doaj.org/toc/2041-1723Tubulin post-translational modifications (PTMs) occur spatiotemporally throughout cells, therefore assessing the physiological roles in specific subcellular compartments has been challenging. Here the authors develop a method to rapidly deplete tubulin glutamylation inside the primary cilia by targeting an engineered deglutamylase to the axoneme.Shi-Rong HongCuei-Ling WangYao-Shen HuangYu-Chen ChangYa-Chu ChangGanesh V. PusapatiChun-Yu LinNing HsuHsiao-Chi ChengYueh-Chen ChiangWei-En HuangNathan C. ShanerRajat RohatgiTakanari InoueYu-Chun LinNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Shi-Rong Hong
Cuei-Ling Wang
Yao-Shen Huang
Yu-Chen Chang
Ya-Chu Chang
Ganesh V. Pusapati
Chun-Yu Lin
Ning Hsu
Hsiao-Chi Cheng
Yueh-Chen Chiang
Wei-En Huang
Nathan C. Shaner
Rajat Rohatgi
Takanari Inoue
Yu-Chun Lin
Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling
description Tubulin post-translational modifications (PTMs) occur spatiotemporally throughout cells, therefore assessing the physiological roles in specific subcellular compartments has been challenging. Here the authors develop a method to rapidly deplete tubulin glutamylation inside the primary cilia by targeting an engineered deglutamylase to the axoneme.
format article
author Shi-Rong Hong
Cuei-Ling Wang
Yao-Shen Huang
Yu-Chen Chang
Ya-Chu Chang
Ganesh V. Pusapati
Chun-Yu Lin
Ning Hsu
Hsiao-Chi Cheng
Yueh-Chen Chiang
Wei-En Huang
Nathan C. Shaner
Rajat Rohatgi
Takanari Inoue
Yu-Chun Lin
author_facet Shi-Rong Hong
Cuei-Ling Wang
Yao-Shen Huang
Yu-Chen Chang
Ya-Chu Chang
Ganesh V. Pusapati
Chun-Yu Lin
Ning Hsu
Hsiao-Chi Cheng
Yueh-Chen Chiang
Wei-En Huang
Nathan C. Shaner
Rajat Rohatgi
Takanari Inoue
Yu-Chun Lin
author_sort Shi-Rong Hong
title Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling
title_short Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling
title_full Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling
title_fullStr Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling
title_full_unstemmed Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling
title_sort spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and hedgehog signaling
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/c1e3bf213f4f48708f15f622d8dd14fd
work_keys_str_mv AT shironghong spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT cueilingwang spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT yaoshenhuang spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT yuchenchang spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT yachuchang spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT ganeshvpusapati spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT chunyulin spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT ninghsu spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT hsiaochicheng spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT yuehchenchiang spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT weienhuang spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT nathancshaner spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT rajatrohatgi spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT takanariinoue spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
AT yuchunlin spatiotemporalmanipulationofciliaryglutamylationrevealsitsrolesinintraciliarytraffickingandhedgehogsignaling
_version_ 1718380516730732544

Ejemplares similares