Effects of surface-modifying ligands on the colloidal stability of ZnO nanoparticle dispersions in in vitro cytotoxicity test media

Dongwook Kwon, Jonghoon Park, Jaehong Park, Seo Yeon Choi, Tae Hyun Yoon Laboratory of Nanoscale Characterization and Environmental Chemistry, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, South Korea Abstract: The extrinsic physicochemical properties o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kwon D, Park J, Choi SY, Yoon TH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c1f09ce4b77f4a3f9cd1413f52e115ad
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c1f09ce4b77f4a3f9cd1413f52e115ad
record_format	dspace
spelling	oai:doaj.org-article:c1f09ce4b77f4a3f9cd1413f52e115ad2021-12-02T08:07:46ZEffects of surface-modifying ligands on the colloidal stability of ZnO nanoparticle dispersions in in vitro cytotoxicity test media1178-2013https://doaj.org/article/c1f09ce4b77f4a3f9cd1413f52e115ad2014-12-01T00:00:00Zhttp://www.dovepress.com/effects-of-surface-modifying-ligands-on-the-colloidal-stability-of-zno-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Dongwook Kwon, Jonghoon Park, Jaehong Park, Seo Yeon Choi, Tae Hyun Yoon Laboratory of Nanoscale Characterization and Environmental Chemistry, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, South Korea Abstract: The extrinsic physicochemical properties of nanoparticles (NPs), such as hydrodynamic size, surface charge, surface functional group, and colloidal stabilities, in toxicity testing media are known to have a significant influence on in vitro toxicity assessments. Therefore, interpretation of nanotoxicity test results should be based on reliable characterization of the NPs’ extrinsic properties in actual toxicity testing media. Here, we present a set of physicochemical characterization results for commercially available ZnO NPs, including core diameter, hydrodynamic diameter, surface charges, and colloidal stabilities, in two in vitro toxicity testing media (Roswell Park Memorial Institute [RPMI] and Dulbecco’s modified Eagle’s medium [DMEM]), as well as simple cell viability assay results for selected ZnO NPs. Four commercially available and manufactured ZnO NPs, with different core sizes, were used in this study, and their surface charge was modified with five different surface coating materials (sodium citrate, tris(2-aminoethyl)amine, poly(acrylic acid), poly(allylamine hydrochloride), and poly-L-lysine hydrochloride). The results showed that ZnO NPs were better dispersed in cell culture media via surface modification with positively or negatively charged molecules. Moreover, in the presence of fetal bovine serum (FBS) in RPMI and DMEM media, ZnO NPs were found even better dispersed for a longer period (at least 48 hours). For the HeLa cells exposed to ZnO NPs in DMEM media without FBS, surface charge-dependent cytotoxicity trends were observed, while these trends were not observed for those cells cultured in FBS-containing media. This confirmed the important roles of surface-modifying compounds and of surface charge on the resultant cytotoxicities of NPs. Keywords: surface modification, agglomeration, sedimentationKwon DPark JPark JChoi SYYoon THDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Supplement 2, Pp 57-65 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kwon D Park J Park J Choi SY Yoon TH Effects of surface-modifying ligands on the colloidal stability of ZnO nanoparticle dispersions in in vitro cytotoxicity test media
description	Dongwook Kwon, Jonghoon Park, Jaehong Park, Seo Yeon Choi, Tae Hyun Yoon Laboratory of Nanoscale Characterization and Environmental Chemistry, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, South Korea Abstract: The extrinsic physicochemical properties of nanoparticles (NPs), such as hydrodynamic size, surface charge, surface functional group, and colloidal stabilities, in toxicity testing media are known to have a significant influence on in vitro toxicity assessments. Therefore, interpretation of nanotoxicity test results should be based on reliable characterization of the NPs’ extrinsic properties in actual toxicity testing media. Here, we present a set of physicochemical characterization results for commercially available ZnO NPs, including core diameter, hydrodynamic diameter, surface charges, and colloidal stabilities, in two in vitro toxicity testing media (Roswell Park Memorial Institute [RPMI] and Dulbecco’s modified Eagle’s medium [DMEM]), as well as simple cell viability assay results for selected ZnO NPs. Four commercially available and manufactured ZnO NPs, with different core sizes, were used in this study, and their surface charge was modified with five different surface coating materials (sodium citrate, tris(2-aminoethyl)amine, poly(acrylic acid), poly(allylamine hydrochloride), and poly-L-lysine hydrochloride). The results showed that ZnO NPs were better dispersed in cell culture media via surface modification with positively or negatively charged molecules. Moreover, in the presence of fetal bovine serum (FBS) in RPMI and DMEM media, ZnO NPs were found even better dispersed for a longer period (at least 48 hours). For the HeLa cells exposed to ZnO NPs in DMEM media without FBS, surface charge-dependent cytotoxicity trends were observed, while these trends were not observed for those cells cultured in FBS-containing media. This confirmed the important roles of surface-modifying compounds and of surface charge on the resultant cytotoxicities of NPs. Keywords: surface modification, agglomeration, sedimentation
format	article
author	Kwon D Park J Park J Choi SY Yoon TH
author_facet	Kwon D Park J Park J Choi SY Yoon TH
author_sort	Kwon D
title	Effects of surface-modifying ligands on the colloidal stability of ZnO nanoparticle dispersions in in vitro cytotoxicity test media
title_short	Effects of surface-modifying ligands on the colloidal stability of ZnO nanoparticle dispersions in in vitro cytotoxicity test media
title_full	Effects of surface-modifying ligands on the colloidal stability of ZnO nanoparticle dispersions in in vitro cytotoxicity test media
title_fullStr	Effects of surface-modifying ligands on the colloidal stability of ZnO nanoparticle dispersions in in vitro cytotoxicity test media
title_full_unstemmed	Effects of surface-modifying ligands on the colloidal stability of ZnO nanoparticle dispersions in in vitro cytotoxicity test media
title_sort	effects of surface-modifying ligands on the colloidal stability of zno nanoparticle dispersions in in vitro cytotoxicity test media
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/c1f09ce4b77f4a3f9cd1413f52e115ad
work_keys_str_mv	AT kwond effectsofsurfacemodifyingligandsonthecolloidalstabilityofznonanoparticledispersionsininvitrocytotoxicitytestmedia AT parkj effectsofsurfacemodifyingligandsonthecolloidalstabilityofznonanoparticledispersionsininvitrocytotoxicitytestmedia AT parkj effectsofsurfacemodifyingligandsonthecolloidalstabilityofznonanoparticledispersionsininvitrocytotoxicitytestmedia AT choisy effectsofsurfacemodifyingligandsonthecolloidalstabilityofznonanoparticledispersionsininvitrocytotoxicitytestmedia AT yoonth effectsofsurfacemodifyingligandsonthecolloidalstabilityofznonanoparticledispersionsininvitrocytotoxicitytestmedia
_version_	1718398686720950272

Effects of surface-modifying ligands on the colloidal stability of ZnO nanoparticle dispersions in in vitro cytotoxicity test media

Ejemplares similares