DNA fingerprinting of Chinese melon provides evidentiary support of seed quality appraisal.

Melon, Cucumis melo L. is an important vegetable crop worldwide. At present, there are phenomena of homonyms and synonyms present in the melon seed markets of China, which could cause variety authenticity issues influencing the process of melon breeding, production, marketing and other aspects. Mole...

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Autores principales:	Peng Gao, Hongyan Ma, Feishi Luan, Haibin Song
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c1f0eb33c65846adae8a7bcc7ff51ebc
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spelling	oai:doaj.org-article:c1f0eb33c65846adae8a7bcc7ff51ebc2021-11-18T08:04:10ZDNA fingerprinting of Chinese melon provides evidentiary support of seed quality appraisal.1932-620310.1371/journal.pone.0052431https://doaj.org/article/c1f0eb33c65846adae8a7bcc7ff51ebc2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23285039/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Melon, Cucumis melo L. is an important vegetable crop worldwide. At present, there are phenomena of homonyms and synonyms present in the melon seed markets of China, which could cause variety authenticity issues influencing the process of melon breeding, production, marketing and other aspects. Molecular markers, especially microsatellites or simple sequence repeats (SSRs) are playing increasingly important roles for cultivar identification. The aim of this study was to construct a DNA fingerprinting database of major melon cultivars, which could provide a possibility for the establishment of a technical standard system for purity and authenticity identification of melon seeds. In this study, to develop the core set SSR markers, 470 polymorphic SSRs were selected as the candidate markers from 1219 SSRs using 20 representative melon varieties (lines). Eighteen SSR markers, evenly distributed across the genome and with the highest contents of polymorphism information (PIC) were identified as the core marker set for melon DNA fingerprinting analysis. Fingerprint codes for 471 melon varieties (lines) were established. There were 51 materials which were classified into17 groups based on sharing the same fingerprint code, while field traits survey results showed that these plants in the same group were synonyms because of the same or similar field characters. Furthermore, DNA fingerprinting quick response (QR) codes of 471 melon varieties (lines) were constructed. Due to its fast readability and large storage capacity, QR coding melon DNA fingerprinting is in favor of read convenience and commercial applications.Peng GaoHongyan MaFeishi LuanHaibin SongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52431 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng Gao Hongyan Ma Feishi Luan Haibin Song DNA fingerprinting of Chinese melon provides evidentiary support of seed quality appraisal.
description	Melon, Cucumis melo L. is an important vegetable crop worldwide. At present, there are phenomena of homonyms and synonyms present in the melon seed markets of China, which could cause variety authenticity issues influencing the process of melon breeding, production, marketing and other aspects. Molecular markers, especially microsatellites or simple sequence repeats (SSRs) are playing increasingly important roles for cultivar identification. The aim of this study was to construct a DNA fingerprinting database of major melon cultivars, which could provide a possibility for the establishment of a technical standard system for purity and authenticity identification of melon seeds. In this study, to develop the core set SSR markers, 470 polymorphic SSRs were selected as the candidate markers from 1219 SSRs using 20 representative melon varieties (lines). Eighteen SSR markers, evenly distributed across the genome and with the highest contents of polymorphism information (PIC) were identified as the core marker set for melon DNA fingerprinting analysis. Fingerprint codes for 471 melon varieties (lines) were established. There were 51 materials which were classified into17 groups based on sharing the same fingerprint code, while field traits survey results showed that these plants in the same group were synonyms because of the same or similar field characters. Furthermore, DNA fingerprinting quick response (QR) codes of 471 melon varieties (lines) were constructed. Due to its fast readability and large storage capacity, QR coding melon DNA fingerprinting is in favor of read convenience and commercial applications.
format	article
author	Peng Gao Hongyan Ma Feishi Luan Haibin Song
author_facet	Peng Gao Hongyan Ma Feishi Luan Haibin Song
author_sort	Peng Gao
title	DNA fingerprinting of Chinese melon provides evidentiary support of seed quality appraisal.
title_short	DNA fingerprinting of Chinese melon provides evidentiary support of seed quality appraisal.
title_full	DNA fingerprinting of Chinese melon provides evidentiary support of seed quality appraisal.
title_fullStr	DNA fingerprinting of Chinese melon provides evidentiary support of seed quality appraisal.
title_full_unstemmed	DNA fingerprinting of Chinese melon provides evidentiary support of seed quality appraisal.
title_sort	dna fingerprinting of chinese melon provides evidentiary support of seed quality appraisal.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c1f0eb33c65846adae8a7bcc7ff51ebc
work_keys_str_mv	AT penggao dnafingerprintingofchinesemelonprovidesevidentiarysupportofseedqualityappraisal AT hongyanma dnafingerprintingofchinesemelonprovidesevidentiarysupportofseedqualityappraisal AT feishiluan dnafingerprintingofchinesemelonprovidesevidentiarysupportofseedqualityappraisal AT haibinsong dnafingerprintingofchinesemelonprovidesevidentiarysupportofseedqualityappraisal
_version_	1718422311909982208

DNA fingerprinting of Chinese melon provides evidentiary support of seed quality appraisal.

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