Functional characteristics of a highly specific integrase encoded by an LTR-retrotransposon.

<h4>Background</h4>The retroviral Integrase protein catalyzes the insertion of linear viral DNA into host cell DNA. Although different retroviruses have been shown to target distinctive chromosomal regions, few of them display a site-specific integration. ZAM, a retroelement from Drosoph...

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Autores principales:	Babacar Faye, Frederick Arnaud, Eric Peyretaillade, Emilie Brasset, Bernard Dastugue, Chantal Vaury
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c1f3b6f9febb47e2a59eb8c3f7a29c41
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spelling	oai:doaj.org-article:c1f3b6f9febb47e2a59eb8c3f7a29c412021-11-25T06:18:39ZFunctional characteristics of a highly specific integrase encoded by an LTR-retrotransposon.1932-620310.1371/journal.pone.0003185https://doaj.org/article/c1f3b6f9febb47e2a59eb8c3f7a29c412008-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18784842/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The retroviral Integrase protein catalyzes the insertion of linear viral DNA into host cell DNA. Although different retroviruses have been shown to target distinctive chromosomal regions, few of them display a site-specific integration. ZAM, a retroelement from Drosophila melanogaster very similar in structure and replication cycle to mammalian retroviruses is highly site-specific. Indeed, ZAM copies target the genomic 5'-CGCGCg-3' consensus-sequences. To enlighten the determinants of this high integration specificity, we investigated the functional properties of its integrase protein denoted ZAM-IN.<h4>Principal findings</h4>Here we show that ZAM-IN displays the property to nick DNA molecules in vitro. This endonuclease activity targets specific sequences that are present in a 388 bp fragment taken from the white locus and known to be a genomic ZAM integration site in vivo. Furthermore, ZAM-IN displays the unusual property to directly bind specific genomic DNA sequences. Two specific and independent sites are recognized within the 388 bp fragment of the white locus: the CGCGCg sequence and a closely apposed site different in sequence.<h4>Conclusion</h4>This study strongly argues that the intrinsic properties of ZAM-IN, ie its binding properties and its endonuclease activity, play an important part in ZAM integration specificity. Its ability to select two binding sites and to nick the DNA molecule reminds the strategy used by some site-specific recombination enzymes and forms the basis for site-specific integration strategies potentially useful in a broad range of genetic engineering applications.Babacar FayeFrederick ArnaudEric PeyretailladeEmilie BrassetBernard DastugueChantal VauryPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 9, p e3185 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Babacar Faye Frederick Arnaud Eric Peyretaillade Emilie Brasset Bernard Dastugue Chantal Vaury Functional characteristics of a highly specific integrase encoded by an LTR-retrotransposon.
description	<h4>Background</h4>The retroviral Integrase protein catalyzes the insertion of linear viral DNA into host cell DNA. Although different retroviruses have been shown to target distinctive chromosomal regions, few of them display a site-specific integration. ZAM, a retroelement from Drosophila melanogaster very similar in structure and replication cycle to mammalian retroviruses is highly site-specific. Indeed, ZAM copies target the genomic 5'-CGCGCg-3' consensus-sequences. To enlighten the determinants of this high integration specificity, we investigated the functional properties of its integrase protein denoted ZAM-IN.<h4>Principal findings</h4>Here we show that ZAM-IN displays the property to nick DNA molecules in vitro. This endonuclease activity targets specific sequences that are present in a 388 bp fragment taken from the white locus and known to be a genomic ZAM integration site in vivo. Furthermore, ZAM-IN displays the unusual property to directly bind specific genomic DNA sequences. Two specific and independent sites are recognized within the 388 bp fragment of the white locus: the CGCGCg sequence and a closely apposed site different in sequence.<h4>Conclusion</h4>This study strongly argues that the intrinsic properties of ZAM-IN, ie its binding properties and its endonuclease activity, play an important part in ZAM integration specificity. Its ability to select two binding sites and to nick the DNA molecule reminds the strategy used by some site-specific recombination enzymes and forms the basis for site-specific integration strategies potentially useful in a broad range of genetic engineering applications.
format	article
author	Babacar Faye Frederick Arnaud Eric Peyretaillade Emilie Brasset Bernard Dastugue Chantal Vaury
author_facet	Babacar Faye Frederick Arnaud Eric Peyretaillade Emilie Brasset Bernard Dastugue Chantal Vaury
author_sort	Babacar Faye
title	Functional characteristics of a highly specific integrase encoded by an LTR-retrotransposon.
title_short	Functional characteristics of a highly specific integrase encoded by an LTR-retrotransposon.
title_full	Functional characteristics of a highly specific integrase encoded by an LTR-retrotransposon.
title_fullStr	Functional characteristics of a highly specific integrase encoded by an LTR-retrotransposon.
title_full_unstemmed	Functional characteristics of a highly specific integrase encoded by an LTR-retrotransposon.
title_sort	functional characteristics of a highly specific integrase encoded by an ltr-retrotransposon.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/c1f3b6f9febb47e2a59eb8c3f7a29c41
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Functional characteristics of a highly specific integrase encoded by an LTR-retrotransposon.

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