Circ-ATAD1 is overexpressed in osteosarcoma (OS) and suppresses the maturation of miR-154-5p to increase cell invasion and migration

Abstract Background Circ-ATAD1 plays an oncogenic role in gastric cancer. However, its roles in other cancers are unclear. We aimed to analyze the role of circ-ATAD1 in osteosarcoma (OS). Methods The expression levels of circ-ATAD1, mature miR-154-5p, and premature miR-154-5p in paired OS and non-tu...

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Autores principales:	Jihui Zhou, Li Xu, Peng Yang, Shibang Lin, Haizhou Huang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Osteosarcoma Circ-ATAD1 miR-154-5p Maturation Migration Invasion Orthopedic surgery RD701-811 Diseases of the musculoskeletal system RC925-935
Acceso en línea:	https://doaj.org/article/c1f5c7f0289243b0b35a02e7cf9145c8
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spelling	oai:doaj.org-article:c1f5c7f0289243b0b35a02e7cf9145c82021-12-05T12:19:21ZCirc-ATAD1 is overexpressed in osteosarcoma (OS) and suppresses the maturation of miR-154-5p to increase cell invasion and migration10.1186/s13018-021-02809-41749-799Xhttps://doaj.org/article/c1f5c7f0289243b0b35a02e7cf9145c82021-12-01T00:00:00Zhttps://doi.org/10.1186/s13018-021-02809-4https://doaj.org/toc/1749-799XAbstract Background Circ-ATAD1 plays an oncogenic role in gastric cancer. However, its roles in other cancers are unclear. We aimed to analyze the role of circ-ATAD1 in osteosarcoma (OS). Methods The expression levels of circ-ATAD1, mature miR-154-5p, and premature miR-154-5p in paired OS and non-tumor tissues from 56 OS patients were determined using RT-qPCR. Nuclear fractionation assay was performed to analyze the subcellular location of circ-ATAD1. The interaction between circ-ATAD1 and premature miR-154-5p was analyzed using RNA pull-down assay. The role of circ-ATAD1 in regulating miR-154-5p maturation was analyzed using RT-qPCR in cells with overexpression. Transwell assays were performed to analyze the roles of circ-ATAD1 and miR-154-5p in regulating OS cell invasion and migration. Results Circ-ATAD1 was overexpressed in OS compared to non-tumor tissues and was detected in the nuclei of OS cells. Mature miR-154-5p, but not premature miR-154-5p, was downregulated in OS tissues compared to non-tumor tissues and was inversely correlated with circ-ATAD1. In OS cells, circ-ATAD1 overexpression decreased the expression of mature miR-154-5p, but not premature miR-154-5p. Transwell assay analysis showed that circ-ATAD1 overexpression increased cell invasion and migration, and mature miR-154-5p overexpression suppressed these cell behaviors. In addition, circ-ATAD1 overexpression reduced the effects of mature miR-154-5p overexpression on cell behaviors. Conclusions Circ-ATAD1 is overexpressed in OS and suppresses miR-154-5p maturation to increase cell invasion and migration.Jihui ZhouLi XuPeng YangShibang LinHaizhou HuangBMCarticleOsteosarcomaCirc-ATAD1miR-154-5pMaturationMigrationInvasionOrthopedic surgeryRD701-811Diseases of the musculoskeletal systemRC925-935ENJournal of Orthopaedic Surgery and Research, Vol 16, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Osteosarcoma Circ-ATAD1 miR-154-5p Maturation Migration Invasion Orthopedic surgery RD701-811 Diseases of the musculoskeletal system RC925-935
spellingShingle	Osteosarcoma Circ-ATAD1 miR-154-5p Maturation Migration Invasion Orthopedic surgery RD701-811 Diseases of the musculoskeletal system RC925-935 Jihui Zhou Li Xu Peng Yang Shibang Lin Haizhou Huang Circ-ATAD1 is overexpressed in osteosarcoma (OS) and suppresses the maturation of miR-154-5p to increase cell invasion and migration
description	Abstract Background Circ-ATAD1 plays an oncogenic role in gastric cancer. However, its roles in other cancers are unclear. We aimed to analyze the role of circ-ATAD1 in osteosarcoma (OS). Methods The expression levels of circ-ATAD1, mature miR-154-5p, and premature miR-154-5p in paired OS and non-tumor tissues from 56 OS patients were determined using RT-qPCR. Nuclear fractionation assay was performed to analyze the subcellular location of circ-ATAD1. The interaction between circ-ATAD1 and premature miR-154-5p was analyzed using RNA pull-down assay. The role of circ-ATAD1 in regulating miR-154-5p maturation was analyzed using RT-qPCR in cells with overexpression. Transwell assays were performed to analyze the roles of circ-ATAD1 and miR-154-5p in regulating OS cell invasion and migration. Results Circ-ATAD1 was overexpressed in OS compared to non-tumor tissues and was detected in the nuclei of OS cells. Mature miR-154-5p, but not premature miR-154-5p, was downregulated in OS tissues compared to non-tumor tissues and was inversely correlated with circ-ATAD1. In OS cells, circ-ATAD1 overexpression decreased the expression of mature miR-154-5p, but not premature miR-154-5p. Transwell assay analysis showed that circ-ATAD1 overexpression increased cell invasion and migration, and mature miR-154-5p overexpression suppressed these cell behaviors. In addition, circ-ATAD1 overexpression reduced the effects of mature miR-154-5p overexpression on cell behaviors. Conclusions Circ-ATAD1 is overexpressed in OS and suppresses miR-154-5p maturation to increase cell invasion and migration.
format	article
author	Jihui Zhou Li Xu Peng Yang Shibang Lin Haizhou Huang
author_facet	Jihui Zhou Li Xu Peng Yang Shibang Lin Haizhou Huang
author_sort	Jihui Zhou
title	Circ-ATAD1 is overexpressed in osteosarcoma (OS) and suppresses the maturation of miR-154-5p to increase cell invasion and migration
title_short	Circ-ATAD1 is overexpressed in osteosarcoma (OS) and suppresses the maturation of miR-154-5p to increase cell invasion and migration
title_full	Circ-ATAD1 is overexpressed in osteosarcoma (OS) and suppresses the maturation of miR-154-5p to increase cell invasion and migration
title_fullStr	Circ-ATAD1 is overexpressed in osteosarcoma (OS) and suppresses the maturation of miR-154-5p to increase cell invasion and migration
title_full_unstemmed	Circ-ATAD1 is overexpressed in osteosarcoma (OS) and suppresses the maturation of miR-154-5p to increase cell invasion and migration
title_sort	circ-atad1 is overexpressed in osteosarcoma (os) and suppresses the maturation of mir-154-5p to increase cell invasion and migration
publisher	BMC
publishDate	2021
url	https://doaj.org/article/c1f5c7f0289243b0b35a02e7cf9145c8
work_keys_str_mv	AT jihuizhou circatad1isoverexpressedinosteosarcomaosandsuppressesthematurationofmir1545ptoincreasecellinvasionandmigration AT lixu circatad1isoverexpressedinosteosarcomaosandsuppressesthematurationofmir1545ptoincreasecellinvasionandmigration AT pengyang circatad1isoverexpressedinosteosarcomaosandsuppressesthematurationofmir1545ptoincreasecellinvasionandmigration AT shibanglin circatad1isoverexpressedinosteosarcomaosandsuppressesthematurationofmir1545ptoincreasecellinvasionandmigration AT haizhouhuang circatad1isoverexpressedinosteosarcomaosandsuppressesthematurationofmir1545ptoincreasecellinvasionandmigration
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Circ-ATAD1 is overexpressed in osteosarcoma (OS) and suppresses the maturation of miR-154-5p to increase cell invasion and migration

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