Acid Dentin Lysate Modulates Macrophage Polarization and Osteoclastogenesis In Vitro

Dentin prepared from extracted teeth is used as autograft for alveolar bone augmentation. Graft consolidation involves the acid lysis of dentin thereby generating a characteristic paracrine environment. Acid lysate of dentin is mimicking this environment. Acid dentin lysate (ADL) potentially targets...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jila Nasirzade, Zahra Kargarpour, Layla Panahipour, Reinhard Gruber
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	acid dentin lysate macrophage osteoclast inflammation Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
Acceso en línea:	https://doaj.org/article/c1f631f9746f4382be338e9b2d84d5e9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c1f631f9746f4382be338e9b2d84d5e9
record_format	dspace
spelling	oai:doaj.org-article:c1f631f9746f4382be338e9b2d84d5e92021-11-25T18:14:43ZAcid Dentin Lysate Modulates Macrophage Polarization and Osteoclastogenesis In Vitro10.3390/ma142269201996-1944https://doaj.org/article/c1f631f9746f4382be338e9b2d84d5e92021-11-01T00:00:00Zhttps://www.mdpi.com/1996-1944/14/22/6920https://doaj.org/toc/1996-1944Dentin prepared from extracted teeth is used as autograft for alveolar bone augmentation. Graft consolidation involves the acid lysis of dentin thereby generating a characteristic paracrine environment. Acid lysate of dentin is mimicking this environment. Acid dentin lysate (ADL) potentially targets hematopoietic cells thereby affecting their differentiation towards macrophages and osteoclasts; however, the question remains if ADL controls macrophage polarization and osteoclastogenesis. Here, we show that ADL reduced lipopolysaccharide (LPS)-induced macrophage polarization of the pro-inflammatory (M1) phenotype, indicated by attenuated Interleukin 1 (IL1), Interleukine 6 (IL6)and cyclooxygenase 2 (COX2) expression. This decrease in M1 macrophages was confirmed by the reduced phosphorylation and nuclear translocation of p65 in the LPS-exposed RAW 264.7 macrophages. Similarly, when RAW 264.7 macrophages were incubated with other agonists of Toll-like receptor (TLR) signaling e.g., FSL1, Polyinosinic-polycytidylic acid High Molecular Weight (Poly (1:C) HMW), Pam3CSK4, and imiquimod, ADL reduced the IL6 expression. We further show herein that ADL decreased osteoclastogenesis indicated by the reduced formation of multinucleated cell expressing cathepsin K and tartrate-resistant acid phosphatase in murine bone marrow cultures. Overall, our results suggest that acid dentin lysate can affect the differentiation of hematopoietic cells to M1 macrophage polarization and a decrease in osteoclastogenesis in bone marrow cultures.Jila NasirzadeZahra KargarpourLayla PanahipourReinhard GruberMDPI AGarticleacid dentin lysatemacrophageosteoclastinflammationTechnologyTElectrical engineering. Electronics. Nuclear engineeringTK1-9971Engineering (General). Civil engineering (General)TA1-2040MicroscopyQH201-278.5Descriptive and experimental mechanicsQC120-168.85ENMaterials, Vol 14, Iss 6920, p 6920 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acid dentin lysate macrophage osteoclast inflammation Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
spellingShingle	acid dentin lysate macrophage osteoclast inflammation Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 Jila Nasirzade Zahra Kargarpour Layla Panahipour Reinhard Gruber Acid Dentin Lysate Modulates Macrophage Polarization and Osteoclastogenesis In Vitro
description	Dentin prepared from extracted teeth is used as autograft for alveolar bone augmentation. Graft consolidation involves the acid lysis of dentin thereby generating a characteristic paracrine environment. Acid lysate of dentin is mimicking this environment. Acid dentin lysate (ADL) potentially targets hematopoietic cells thereby affecting their differentiation towards macrophages and osteoclasts; however, the question remains if ADL controls macrophage polarization and osteoclastogenesis. Here, we show that ADL reduced lipopolysaccharide (LPS)-induced macrophage polarization of the pro-inflammatory (M1) phenotype, indicated by attenuated Interleukin 1 (IL1), Interleukine 6 (IL6)and cyclooxygenase 2 (COX2) expression. This decrease in M1 macrophages was confirmed by the reduced phosphorylation and nuclear translocation of p65 in the LPS-exposed RAW 264.7 macrophages. Similarly, when RAW 264.7 macrophages were incubated with other agonists of Toll-like receptor (TLR) signaling e.g., FSL1, Polyinosinic-polycytidylic acid High Molecular Weight (Poly (1:C) HMW), Pam3CSK4, and imiquimod, ADL reduced the IL6 expression. We further show herein that ADL decreased osteoclastogenesis indicated by the reduced formation of multinucleated cell expressing cathepsin K and tartrate-resistant acid phosphatase in murine bone marrow cultures. Overall, our results suggest that acid dentin lysate can affect the differentiation of hematopoietic cells to M1 macrophage polarization and a decrease in osteoclastogenesis in bone marrow cultures.
format	article
author	Jila Nasirzade Zahra Kargarpour Layla Panahipour Reinhard Gruber
author_facet	Jila Nasirzade Zahra Kargarpour Layla Panahipour Reinhard Gruber
author_sort	Jila Nasirzade
title	Acid Dentin Lysate Modulates Macrophage Polarization and Osteoclastogenesis In Vitro
title_short	Acid Dentin Lysate Modulates Macrophage Polarization and Osteoclastogenesis In Vitro
title_full	Acid Dentin Lysate Modulates Macrophage Polarization and Osteoclastogenesis In Vitro
title_fullStr	Acid Dentin Lysate Modulates Macrophage Polarization and Osteoclastogenesis In Vitro
title_full_unstemmed	Acid Dentin Lysate Modulates Macrophage Polarization and Osteoclastogenesis In Vitro
title_sort	acid dentin lysate modulates macrophage polarization and osteoclastogenesis in vitro
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c1f631f9746f4382be338e9b2d84d5e9
work_keys_str_mv	AT jilanasirzade aciddentinlysatemodulatesmacrophagepolarizationandosteoclastogenesisinvitro AT zahrakargarpour aciddentinlysatemodulatesmacrophagepolarizationandosteoclastogenesisinvitro AT laylapanahipour aciddentinlysatemodulatesmacrophagepolarizationandosteoclastogenesisinvitro AT reinhardgruber aciddentinlysatemodulatesmacrophagepolarizationandosteoclastogenesisinvitro
_version_	1718411462684180480

Acid Dentin Lysate Modulates Macrophage Polarization and Osteoclastogenesis In Vitro

Ejemplares similares