Impact of Different Oxygen Supply Methods on the Healing of Corneal Epithelial Wound and the Level of Acetylcholine

Purpose. To investigate the impact of different oxygen supply methods on corneal epithelial wound healing and acetylcholine level during wound healing. Methods. We randomly divided 75 rabbits into three groups: A, B, and C, with 25 rabbits in each group. The central corneal epithelium was removed fr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shanshan Li, Gang Ding, Yuqin Sun, Chenming Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/c1fed9fe3d4d47ddb667dc3b19dd21fa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c1fed9fe3d4d47ddb667dc3b19dd21fa
record_format	dspace
spelling	oai:doaj.org-article:c1fed9fe3d4d47ddb667dc3b19dd21fa2021-11-29T00:55:53ZImpact of Different Oxygen Supply Methods on the Healing of Corneal Epithelial Wound and the Level of Acetylcholine2090-005810.1155/2021/4737479https://doaj.org/article/c1fed9fe3d4d47ddb667dc3b19dd21fa2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/4737479https://doaj.org/toc/2090-0058Purpose. To investigate the impact of different oxygen supply methods on corneal epithelial wound healing and acetylcholine level during wound healing. Methods. We randomly divided 75 rabbits into three groups: A, B, and C, with 25 rabbits in each group. The central corneal epithelium was removed from all eyes of the rabbits using a 5 mm trephine. Group A rabbits were given low flow oxygen (3 L/min; concentration: 33%) for 2 h per day through goggles. Group B rabbits were given low flow oxygen (3 L/min; concentration: 33%) for 2 h per day via oxygen masks for inhalation. Group C rabbits healed naturally. The area of healed corneal epithelium and acetylcholine content in corneal epithelium were determined at 12 h, 24 h, and 36 h after injury. Results. At 12 h, 24 h, and 36 h after injury, the healing area of corneal epithelium in the three groups was in the order group A > group B > group C (P<0.05). At all timepoints, the acetylcholine level in corneal epithelium was in the order of group A > group B > group C (P<0.05). In all three groups, the acetylcholine content in corneal epithelium showed the order 12 h > 24 h > 36 h (P<0.05). There was a correlation between acetylcholine expression and the area of unhealed corneal epithelium, and the correlation coefficients of groups A, B, and C were 0.80, 0.83, and 0.85 respectively. Conclusion. Increasing oxygen concentration through inhalation or via goggles can promote corneal epithelial wound healing, but increasing local oxygen concentration of the eye showed a better effect. Acetylcholine may play an important role in the early process of corneal epithelial wound healing.Shanshan LiGang DingYuqin SunChenming ZhangHindawi LimitedarticleOphthalmologyRE1-994ENJournal of Ophthalmology, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Shanshan Li Gang Ding Yuqin Sun Chenming Zhang Impact of Different Oxygen Supply Methods on the Healing of Corneal Epithelial Wound and the Level of Acetylcholine
description	Purpose. To investigate the impact of different oxygen supply methods on corneal epithelial wound healing and acetylcholine level during wound healing. Methods. We randomly divided 75 rabbits into three groups: A, B, and C, with 25 rabbits in each group. The central corneal epithelium was removed from all eyes of the rabbits using a 5 mm trephine. Group A rabbits were given low flow oxygen (3 L/min; concentration: 33%) for 2 h per day through goggles. Group B rabbits were given low flow oxygen (3 L/min; concentration: 33%) for 2 h per day via oxygen masks for inhalation. Group C rabbits healed naturally. The area of healed corneal epithelium and acetylcholine content in corneal epithelium were determined at 12 h, 24 h, and 36 h after injury. Results. At 12 h, 24 h, and 36 h after injury, the healing area of corneal epithelium in the three groups was in the order group A > group B > group C (P<0.05). At all timepoints, the acetylcholine level in corneal epithelium was in the order of group A > group B > group C (P<0.05). In all three groups, the acetylcholine content in corneal epithelium showed the order 12 h > 24 h > 36 h (P<0.05). There was a correlation between acetylcholine expression and the area of unhealed corneal epithelium, and the correlation coefficients of groups A, B, and C were 0.80, 0.83, and 0.85 respectively. Conclusion. Increasing oxygen concentration through inhalation or via goggles can promote corneal epithelial wound healing, but increasing local oxygen concentration of the eye showed a better effect. Acetylcholine may play an important role in the early process of corneal epithelial wound healing.
format	article
author	Shanshan Li Gang Ding Yuqin Sun Chenming Zhang
author_facet	Shanshan Li Gang Ding Yuqin Sun Chenming Zhang
author_sort	Shanshan Li
title	Impact of Different Oxygen Supply Methods on the Healing of Corneal Epithelial Wound and the Level of Acetylcholine
title_short	Impact of Different Oxygen Supply Methods on the Healing of Corneal Epithelial Wound and the Level of Acetylcholine
title_full	Impact of Different Oxygen Supply Methods on the Healing of Corneal Epithelial Wound and the Level of Acetylcholine
title_fullStr	Impact of Different Oxygen Supply Methods on the Healing of Corneal Epithelial Wound and the Level of Acetylcholine
title_full_unstemmed	Impact of Different Oxygen Supply Methods on the Healing of Corneal Epithelial Wound and the Level of Acetylcholine
title_sort	impact of different oxygen supply methods on the healing of corneal epithelial wound and the level of acetylcholine
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/c1fed9fe3d4d47ddb667dc3b19dd21fa
work_keys_str_mv	AT shanshanli impactofdifferentoxygensupplymethodsonthehealingofcornealepithelialwoundandthelevelofacetylcholine AT gangding impactofdifferentoxygensupplymethodsonthehealingofcornealepithelialwoundandthelevelofacetylcholine AT yuqinsun impactofdifferentoxygensupplymethodsonthehealingofcornealepithelialwoundandthelevelofacetylcholine AT chenmingzhang impactofdifferentoxygensupplymethodsonthehealingofcornealepithelialwoundandthelevelofacetylcholine
_version_	1718407795482558464

Impact of Different Oxygen Supply Methods on the Healing of Corneal Epithelial Wound and the Level of Acetylcholine

Ejemplares similares