Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes

Background: Genetic information is necessary to devise strategic plans aimed to improve the genetic merit of buffalos. Objective: To assess the effect of genetic polymorphisms in GH, Pit-1, GHR, GHRHR, and KCN3 genes on milk production and body weight of Khuzestan water buffaloes. Methods: Blood sam...

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Autores principales:	Meysam Ahmadzadeh, Farzad Rashidi, Hamed Amirpour Najafabadi, Amir Jaferian, Shahin Eghbalsaied
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2019
Materias:	animal breeding casein genetic variation growth hormone milk production snp Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/c20c5ee4f134409fb2f54f5acecaa684
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spelling	oai:doaj.org-article:c20c5ee4f134409fb2f54f5acecaa6842021-12-01T18:59:21ZEffects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes2256-295810.17533/udea.rccp.v32n2a04https://doaj.org/article/c20c5ee4f134409fb2f54f5acecaa6842019-05-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/335848https://doaj.org/toc/2256-2958Background: Genetic information is necessary to devise strategic plans aimed to improve the genetic merit of buffalos. Objective: To assess the effect of genetic polymorphisms in GH, Pit-1, GHR, GHRHR, and KCN3 genes on milk production and body weight of Khuzestan water buffaloes. Methods: Blood samples were collected from 60 buffaloes from the Khuzestan province, Iran. Using the PCR-RFLP technique, the amplified and digested fragments of GH/AluI, GHR/AluI, GHRHR/ HaeIII, Pit1/HinfI, and KCN3/HindIII were genotyped. Results: All animals were monomorphic for GHRHR. The frequency of mutant alleles for GH, GHR, KCN3, and Pit1 was 47.5, 74.2, 49.2, and 51.7%, respectively. There were significant differences (p<0.0001) in the genotypic frequencies of GH, GHR, and Pit1 between high and low milk-yielding buffaloes. The GH (p=0.0002), GHR (p<0.0001) and Pit1 (p<0.0001) polymorphisms also had significant effects on body weight. Sequencing results revealed the presence of C496A, G495A, G498A and C1501T SNPs in the GH, and G1702T in the GHR gene of Khuzestan buffalos. Conclusion: This study highlights the importance of GH, GHR, and Pit1 on milk production and body weight of Khuzestan buffaloes. The results suggest that devising an integrated breeding plan in Khuzestan water buffalos can considerably benefit from the very high diversity in candidate genes.Meysam AhmadzadehFarzad RashidiHamed Amirpour NajafabadiAmir JaferianShahin EghbalsaiedUniversidad de Antioquiaarticleanimal breedingcaseingenetic variationgrowth hormonemilk productionsnpAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 32, Iss 2, Pp 107-116 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	animal breeding casein genetic variation growth hormone milk production snp Animal culture SF1-1100
spellingShingle	animal breeding casein genetic variation growth hormone milk production snp Animal culture SF1-1100 Meysam Ahmadzadeh Farzad Rashidi Hamed Amirpour Najafabadi Amir Jaferian Shahin Eghbalsaied Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes
description	Background: Genetic information is necessary to devise strategic plans aimed to improve the genetic merit of buffalos. Objective: To assess the effect of genetic polymorphisms in GH, Pit-1, GHR, GHRHR, and KCN3 genes on milk production and body weight of Khuzestan water buffaloes. Methods: Blood samples were collected from 60 buffaloes from the Khuzestan province, Iran. Using the PCR-RFLP technique, the amplified and digested fragments of GH/AluI, GHR/AluI, GHRHR/ HaeIII, Pit1/HinfI, and KCN3/HindIII were genotyped. Results: All animals were monomorphic for GHRHR. The frequency of mutant alleles for GH, GHR, KCN3, and Pit1 was 47.5, 74.2, 49.2, and 51.7%, respectively. There were significant differences (p<0.0001) in the genotypic frequencies of GH, GHR, and Pit1 between high and low milk-yielding buffaloes. The GH (p=0.0002), GHR (p<0.0001) and Pit1 (p<0.0001) polymorphisms also had significant effects on body weight. Sequencing results revealed the presence of C496A, G495A, G498A and C1501T SNPs in the GH, and G1702T in the GHR gene of Khuzestan buffalos. Conclusion: This study highlights the importance of GH, GHR, and Pit1 on milk production and body weight of Khuzestan buffaloes. The results suggest that devising an integrated breeding plan in Khuzestan water buffalos can considerably benefit from the very high diversity in candidate genes.
format	article
author	Meysam Ahmadzadeh Farzad Rashidi Hamed Amirpour Najafabadi Amir Jaferian Shahin Eghbalsaied
author_facet	Meysam Ahmadzadeh Farzad Rashidi Hamed Amirpour Najafabadi Amir Jaferian Shahin Eghbalsaied
author_sort	Meysam Ahmadzadeh
title	Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes
title_short	Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes
title_full	Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes
title_fullStr	Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes
title_full_unstemmed	Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes
title_sort	effects of genetic polymorphism in pit1, gh, ghr and kcn3 on milk yield and body weight of khuzestan (iran) water buffaloes
publisher	Universidad de Antioquia
publishDate	2019
url	https://doaj.org/article/c20c5ee4f134409fb2f54f5acecaa684
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Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes

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