Profiling extra cellular matrix associated proteome of human fetal nucleus pulposus in search for regenerative targets

Abstract Degeneration of the intervertebral disc is associated with a decrease in extra-cellular matrix (ECM) content due to an imbalance in anabolic and catabolic signaling. Our previous study profiled the core matrisome of fetal NP’s and identified various proteins with anabolic potential for rege...

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Autores principales:	Shanmuganathan Rajasekaran, Chitra Thangavel, Niek Djuric, Muthurajan Raveendran, Dilip Chand Raja Soundararajan, Sharon Miracle Nayagam, Monica Steffi Matchado, K. S. Sri Vijay Anand, Krishna Venkateshwaran
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c20e01486570491686ead66709ff3f57
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spelling	oai:doaj.org-article:c20e01486570491686ead66709ff3f572021-12-02T18:14:39ZProfiling extra cellular matrix associated proteome of human fetal nucleus pulposus in search for regenerative targets10.1038/s41598-021-97620-w2045-2322https://doaj.org/article/c20e01486570491686ead66709ff3f572021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97620-whttps://doaj.org/toc/2045-2322Abstract Degeneration of the intervertebral disc is associated with a decrease in extra-cellular matrix (ECM) content due to an imbalance in anabolic and catabolic signaling. Our previous study profiled the core matrisome of fetal NP’s and identified various proteins with anabolic potential for regenerative therapies. This study aims to complement those results by exploring ECM regulators, associated proteins and secreted factors of the fetal nucleus pulposus (NP). Proteomic data of 9 fetal, 7 healthy adults (age 22–79), and 11 degenerated NP’s was analyzed. Based on the selection criteria, a total of 45 proteins were identified, of which 14 were uniquely expressed or upregulated in fetus compared to adult NP’s. Pathway analysis with these proteins revealed a significant upregulation of one pathway and two biological processes, in which 12 proteins were involved. Prolyl 4 hydroxylase (P4HA) 1 and 2, Procollagen-lysine, 2-oxoglutarate 5-dioxygenase (PLOD) 1, and Heat shock protein 47 (SERPINH1) were involved in ‘collagen biosynthesis’ pathway. In addition, PLOD 1, SERPINH1, Annexin A1 and A4, CD109 and Galectin 3 (LGALS3) were all involved in biological process of ‘tissue development’. Furthermore Annexin A1, A4 and A5, LGALS-3 and SERPINF1 were featured in ‘negative regulation of cell death’. In conclusion, additionally to core ECM proteome, this study reveals ECM regulators and ECM affiliated proteins of interest to study for regenerative therapies, and their potential should be validated in future mechanistic experiments.Shanmuganathan RajasekaranChitra ThangavelNiek DjuricMuthurajan RaveendranDilip Chand Raja SoundararajanSharon Miracle NayagamMonica Steffi MatchadoK. S. Sri Vijay AnandKrishna VenkateshwaranNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shanmuganathan Rajasekaran Chitra Thangavel Niek Djuric Muthurajan Raveendran Dilip Chand Raja Soundararajan Sharon Miracle Nayagam Monica Steffi Matchado K. S. Sri Vijay Anand Krishna Venkateshwaran Profiling extra cellular matrix associated proteome of human fetal nucleus pulposus in search for regenerative targets
description	Abstract Degeneration of the intervertebral disc is associated with a decrease in extra-cellular matrix (ECM) content due to an imbalance in anabolic and catabolic signaling. Our previous study profiled the core matrisome of fetal NP’s and identified various proteins with anabolic potential for regenerative therapies. This study aims to complement those results by exploring ECM regulators, associated proteins and secreted factors of the fetal nucleus pulposus (NP). Proteomic data of 9 fetal, 7 healthy adults (age 22–79), and 11 degenerated NP’s was analyzed. Based on the selection criteria, a total of 45 proteins were identified, of which 14 were uniquely expressed or upregulated in fetus compared to adult NP’s. Pathway analysis with these proteins revealed a significant upregulation of one pathway and two biological processes, in which 12 proteins were involved. Prolyl 4 hydroxylase (P4HA) 1 and 2, Procollagen-lysine, 2-oxoglutarate 5-dioxygenase (PLOD) 1, and Heat shock protein 47 (SERPINH1) were involved in ‘collagen biosynthesis’ pathway. In addition, PLOD 1, SERPINH1, Annexin A1 and A4, CD109 and Galectin 3 (LGALS3) were all involved in biological process of ‘tissue development’. Furthermore Annexin A1, A4 and A5, LGALS-3 and SERPINF1 were featured in ‘negative regulation of cell death’. In conclusion, additionally to core ECM proteome, this study reveals ECM regulators and ECM affiliated proteins of interest to study for regenerative therapies, and their potential should be validated in future mechanistic experiments.
format	article
author	Shanmuganathan Rajasekaran Chitra Thangavel Niek Djuric Muthurajan Raveendran Dilip Chand Raja Soundararajan Sharon Miracle Nayagam Monica Steffi Matchado K. S. Sri Vijay Anand Krishna Venkateshwaran
author_facet	Shanmuganathan Rajasekaran Chitra Thangavel Niek Djuric Muthurajan Raveendran Dilip Chand Raja Soundararajan Sharon Miracle Nayagam Monica Steffi Matchado K. S. Sri Vijay Anand Krishna Venkateshwaran
author_sort	Shanmuganathan Rajasekaran
title	Profiling extra cellular matrix associated proteome of human fetal nucleus pulposus in search for regenerative targets
title_short	Profiling extra cellular matrix associated proteome of human fetal nucleus pulposus in search for regenerative targets
title_full	Profiling extra cellular matrix associated proteome of human fetal nucleus pulposus in search for regenerative targets
title_fullStr	Profiling extra cellular matrix associated proteome of human fetal nucleus pulposus in search for regenerative targets
title_full_unstemmed	Profiling extra cellular matrix associated proteome of human fetal nucleus pulposus in search for regenerative targets
title_sort	profiling extra cellular matrix associated proteome of human fetal nucleus pulposus in search for regenerative targets
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c20e01486570491686ead66709ff3f57
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Profiling extra cellular matrix associated proteome of human fetal nucleus pulposus in search for regenerative targets

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