Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly

Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that scaffolds organelle formation. Here, the authors develop monobodies against Chlamydomonas reinhardtii SAS-6 and use X-ray crystallography, atomic force micro...

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Autores principales:	Georgios N. Hatzopoulos, Tim Kükenshöner, Niccolò Banterle, Tatiana Favez, Isabelle Flückiger, Virginie Hamel, Santiago Andany, Georg E. Fantner, Oliver Hantschel, Pierre Gönczy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c2111ed0fd0843e78837713fbdafff46
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spelling	oai:doaj.org-article:c2111ed0fd0843e78837713fbdafff462021-12-02T17:14:22ZTuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly10.1038/s41467-021-23897-02041-1723https://doaj.org/article/c2111ed0fd0843e78837713fbdafff462021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23897-0https://doaj.org/toc/2041-1723Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that scaffolds organelle formation. Here, the authors develop monobodies against Chlamydomonas reinhardtii SAS-6 and use X-ray crystallography, atomic force microscopy and cryo-electron microscopy to reveal insights into ring assembly and stacking.Georgios N. HatzopoulosTim KükenshönerNiccolò BanterleTatiana FavezIsabelle FlückigerVirginie HamelSantiago AndanyGeorg E. FantnerOliver HantschelPierre GönczyNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Georgios N. Hatzopoulos Tim Kükenshöner Niccolò Banterle Tatiana Favez Isabelle Flückiger Virginie Hamel Santiago Andany Georg E. Fantner Oliver Hantschel Pierre Gönczy Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly
description	Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that scaffolds organelle formation. Here, the authors develop monobodies against Chlamydomonas reinhardtii SAS-6 and use X-ray crystallography, atomic force microscopy and cryo-electron microscopy to reveal insights into ring assembly and stacking.
format	article
author	Georgios N. Hatzopoulos Tim Kükenshöner Niccolò Banterle Tatiana Favez Isabelle Flückiger Virginie Hamel Santiago Andany Georg E. Fantner Oliver Hantschel Pierre Gönczy
author_facet	Georgios N. Hatzopoulos Tim Kükenshöner Niccolò Banterle Tatiana Favez Isabelle Flückiger Virginie Hamel Santiago Andany Georg E. Fantner Oliver Hantschel Pierre Gönczy
author_sort	Georgios N. Hatzopoulos
title	Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly
title_short	Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly
title_full	Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly
title_fullStr	Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly
title_full_unstemmed	Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly
title_sort	tuning sas-6 architecture with monobodies impairs distinct steps of centriole assembly
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c2111ed0fd0843e78837713fbdafff46
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Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly

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