Comparison of DIA with hemagglutination inhibition (HI) and enzyme immunoassay (EIA) in diagnosis of Measles

Objective: Measles is one of the acute and contagious diseases of human, and according to the estimation of the world health organization (WHO), it may be eradicated by the year 2010. Therefore, diagnosis of measles is emphasized by using quick and sensitive methods for control and prevention of mea...

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Autores principales:	Y Yahya Pour, T Mokhtari Azad, M Mahmoudi, M Pezeshki, R Nategh
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2001
Materias:	dot-immunoassay hemagglutination inhibition elisa measles Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c217a7552a7041bdb8aa99447d69b4da
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id	oai:doaj.org-article:c217a7552a7041bdb8aa99447d69b4da
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spelling	oai:doaj.org-article:c217a7552a7041bdb8aa99447d69b4da2021-11-10T09:21:00ZComparison of DIA with hemagglutination inhibition (HI) and enzyme immunoassay (EIA) in diagnosis of Measles1561-41072251-7170https://doaj.org/article/c217a7552a7041bdb8aa99447d69b4da2001-04-01T00:00:00Zhttp://jbums.org/article-1-2869-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170Objective: Measles is one of the acute and contagious diseases of human, and according to the estimation of the world health organization (WHO), it may be eradicated by the year 2010. Therefore, diagnosis of measles is emphasized by using quick and sensitive methods for control and prevention of measles. Methods: Various methods are used to diagnose antibody against measles virus, include hemagglutination inhibition (HI), enzyme immunoassay (EIA), plaque reduction (PR) and neutralization test (NT). One of the methods is dot-immunoassay (DIA). In this method, nitrocellulose paper is used as solid phase instead of microplate. Findings: In this study, DIA in comparison to HI had 98.8% sensitivity and 85% specificity, and in comparison to EIA had 96.5%, 90% sensitivity and specificity respectively. Conclusion: This technique has advantages such as sensitivity, high specificity, low cost and speed.Y Yahya PourT Mokhtari AzadM MahmoudiM PezeshkiR NateghBabol University of Medical Sciencesarticledot-immunoassayhemagglutination inhibitionelisameaslesMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 3, Iss 2, Pp 12-17 (2001)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	dot-immunoassay hemagglutination inhibition elisa measles Medicine R Medicine (General) R5-920
spellingShingle	dot-immunoassay hemagglutination inhibition elisa measles Medicine R Medicine (General) R5-920 Y Yahya Pour T Mokhtari Azad M Mahmoudi M Pezeshki R Nategh Comparison of DIA with hemagglutination inhibition (HI) and enzyme immunoassay (EIA) in diagnosis of Measles
description	Objective: Measles is one of the acute and contagious diseases of human, and according to the estimation of the world health organization (WHO), it may be eradicated by the year 2010. Therefore, diagnosis of measles is emphasized by using quick and sensitive methods for control and prevention of measles. Methods: Various methods are used to diagnose antibody against measles virus, include hemagglutination inhibition (HI), enzyme immunoassay (EIA), plaque reduction (PR) and neutralization test (NT). One of the methods is dot-immunoassay (DIA). In this method, nitrocellulose paper is used as solid phase instead of microplate. Findings: In this study, DIA in comparison to HI had 98.8% sensitivity and 85% specificity, and in comparison to EIA had 96.5%, 90% sensitivity and specificity respectively. Conclusion: This technique has advantages such as sensitivity, high specificity, low cost and speed.
format	article
author	Y Yahya Pour T Mokhtari Azad M Mahmoudi M Pezeshki R Nategh
author_facet	Y Yahya Pour T Mokhtari Azad M Mahmoudi M Pezeshki R Nategh
author_sort	Y Yahya Pour
title	Comparison of DIA with hemagglutination inhibition (HI) and enzyme immunoassay (EIA) in diagnosis of Measles
title_short	Comparison of DIA with hemagglutination inhibition (HI) and enzyme immunoassay (EIA) in diagnosis of Measles
title_full	Comparison of DIA with hemagglutination inhibition (HI) and enzyme immunoassay (EIA) in diagnosis of Measles
title_fullStr	Comparison of DIA with hemagglutination inhibition (HI) and enzyme immunoassay (EIA) in diagnosis of Measles
title_full_unstemmed	Comparison of DIA with hemagglutination inhibition (HI) and enzyme immunoassay (EIA) in diagnosis of Measles
title_sort	comparison of dia with hemagglutination inhibition (hi) and enzyme immunoassay (eia) in diagnosis of measles
publisher	Babol University of Medical Sciences
publishDate	2001
url	https://doaj.org/article/c217a7552a7041bdb8aa99447d69b4da
work_keys_str_mv	AT yyahyapour comparisonofdiawithhemagglutinationinhibitionhiandenzymeimmunoassayeiaindiagnosisofmeasles AT tmokhtariazad comparisonofdiawithhemagglutinationinhibitionhiandenzymeimmunoassayeiaindiagnosisofmeasles AT mmahmoudi comparisonofdiawithhemagglutinationinhibitionhiandenzymeimmunoassayeiaindiagnosisofmeasles AT mpezeshki comparisonofdiawithhemagglutinationinhibitionhiandenzymeimmunoassayeiaindiagnosisofmeasles AT rnategh comparisonofdiawithhemagglutinationinhibitionhiandenzymeimmunoassayeiaindiagnosisofmeasles
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Comparison of DIA with hemagglutination inhibition (HI) and enzyme immunoassay (EIA) in diagnosis of Measles

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