Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein

Understanding how DNA methylation regulates gene expression requires the capacity to deploy it to regions of interest. The authors generate a highly rapid and locus-specific CpG methylation tool by fusing dCas9 to MQ1 DNA methyltransferase and show efficacy at multiple sitesin vitro and in vivo.

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Autores principales:	Yong Lei, Xiaotian Zhang, Jianzhong Su, Mira Jeong, Michael C. Gundry, Yung-Hsin Huang, Yubin Zhou, Wei Li, Margaret A. Goodell
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c2215de867694f8d8d9eb31b2329443a
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spelling	oai:doaj.org-article:c2215de867694f8d8d9eb31b2329443a2021-12-02T15:39:04ZTargeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein10.1038/ncomms160262041-1723https://doaj.org/article/c2215de867694f8d8d9eb31b2329443a2017-07-01T00:00:00Zhttps://doi.org/10.1038/ncomms16026https://doaj.org/toc/2041-1723Understanding how DNA methylation regulates gene expression requires the capacity to deploy it to regions of interest. The authors generate a highly rapid and locus-specific CpG methylation tool by fusing dCas9 to MQ1 DNA methyltransferase and show efficacy at multiple sitesin vitro and in vivo.Yong LeiXiaotian ZhangJianzhong SuMira JeongMichael C. GundryYung-Hsin HuangYubin ZhouWei LiMargaret A. GoodellNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yong Lei Xiaotian Zhang Jianzhong Su Mira Jeong Michael C. Gundry Yung-Hsin Huang Yubin Zhou Wei Li Margaret A. Goodell Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein
description	Understanding how DNA methylation regulates gene expression requires the capacity to deploy it to regions of interest. The authors generate a highly rapid and locus-specific CpG methylation tool by fusing dCas9 to MQ1 DNA methyltransferase and show efficacy at multiple sitesin vitro and in vivo.
format	article
author	Yong Lei Xiaotian Zhang Jianzhong Su Mira Jeong Michael C. Gundry Yung-Hsin Huang Yubin Zhou Wei Li Margaret A. Goodell
author_facet	Yong Lei Xiaotian Zhang Jianzhong Su Mira Jeong Michael C. Gundry Yung-Hsin Huang Yubin Zhou Wei Li Margaret A. Goodell
author_sort	Yong Lei
title	Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein
title_short	Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein
title_full	Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein
title_fullStr	Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein
title_full_unstemmed	Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein
title_sort	targeted dna methylation in vivo using an engineered dcas9-mq1 fusion protein
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c2215de867694f8d8d9eb31b2329443a
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Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein

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