Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein
Understanding how DNA methylation regulates gene expression requires the capacity to deploy it to regions of interest. The authors generate a highly rapid and locus-specific CpG methylation tool by fusing dCas9 to MQ1 DNA methyltransferase and show efficacy at multiple sitesin vitro and in vivo.
Guardado en:
Autores principales: | Yong Lei, Xiaotian Zhang, Jianzhong Su, Mira Jeong, Michael C. Gundry, Yung-Hsin Huang, Yubin Zhou, Wei Li, Margaret A. Goodell |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2017
|
Materias: | |
Acceso en línea: | https://doaj.org/article/c2215de867694f8d8d9eb31b2329443a |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
Targeted DNA methylation in human cells using engineered dCas9-methyltransferases
por: Tina Xiong, et al.
Publicado: (2017) -
In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
por: Beatriz Álvarez, et al.
Publicado: (2020) -
dCas9 regulator to neutralize competition in CRISPRi circuits
por: Hsin-Ho Huang, et al.
Publicado: (2021) -
Visualisation of dCas9 target search in vivo using an open-microscopy framework
por: Koen J. A. Martens, et al.
Publicado: (2019) -
Programmable DNA looping using engineered bivalent dCas9 complexes
por: Nan Hao, et al.
Publicado: (2017)