Bioplastic Production by <i>Bacillus wiedmannii</i> AS-02 OK576278 Using Different Agricultural Wastes

Polyhydroxybutyrates (PHBs) are macromolecules synthesized by bacteria. Because of their fast degradability under natural environmental conditions, PHBs were selected as alternatives for the production of biodegradable plastics. Sixteen PHB-accumulating strains were selected and compared for their a...

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Autores principales:	Amal W. Danial, Shereen M. Hamdy, Sulaiman A. Alrumman, Sanaa M. F. Gad El-Rab, Ahmed A. M. Shoreit, Abd El-Latif Hesham
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Polyhydroxybutyrates (PHBs) agricultural wastes bioplastic bacteria 16S rRNA analysis NMR Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c226598ee9e14bd2807f9f7fb1eab5f7
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spelling	oai:doaj.org-article:c226598ee9e14bd2807f9f7fb1eab5f72021-11-25T18:25:39ZBioplastic Production by <i>Bacillus wiedmannii</i> AS-02 OK576278 Using Different Agricultural Wastes10.3390/microorganisms91123952076-2607https://doaj.org/article/c226598ee9e14bd2807f9f7fb1eab5f72021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2395https://doaj.org/toc/2076-2607Polyhydroxybutyrates (PHBs) are macromolecules synthesized by bacteria. Because of their fast degradability under natural environmental conditions, PHBs were selected as alternatives for the production of biodegradable plastics. Sixteen PHB-accumulating strains were selected and compared for their ability to accumulate PHB granules inside their cells. Isolate AS-02 was isolated from cattle manure and identified as <i>Bacillus wiedmannii</i> AS-02 OK576278 by means of 16S rRNA analysis. It was found to be the best producer. The optimum pH, temperature, and incubation period for the best PHB production by the isolate were 7, 35 °C, and 72 h respectively. PHB production was the best with peptone and glucose as nitrogen and carbon sources at a C/N ratio of (2:1). The strain was able to accumulate 423, 390, 249, 158, and 144 mg/L PHB when pretreated orange, mango, banana, onion peels, and rice straw were used as carbon sources, respectively. The extracted polymer was characterized by Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR), and GC-MS spectroscopy, which confirmed the structure of the polymer as PHB. The isolate <i>B. wiedmannii</i> AS-02 OK576278 can be considered an excellent candidate for industrial production of PHB from agricultural wastes.Amal W. DanialShereen M. HamdySulaiman A. AlrummanSanaa M. F. Gad El-RabAhmed A. M. ShoreitAbd El-Latif HeshamMDPI AGarticlePolyhydroxybutyrates (PHBs)agricultural wastesbioplasticbacteria16S rRNA analysisNMRBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2395, p 2395 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Polyhydroxybutyrates (PHBs) agricultural wastes bioplastic bacteria 16S rRNA analysis NMR Biology (General) QH301-705.5
spellingShingle	Polyhydroxybutyrates (PHBs) agricultural wastes bioplastic bacteria 16S rRNA analysis NMR Biology (General) QH301-705.5 Amal W. Danial Shereen M. Hamdy Sulaiman A. Alrumman Sanaa M. F. Gad El-Rab Ahmed A. M. Shoreit Abd El-Latif Hesham Bioplastic Production by <i>Bacillus wiedmannii</i> AS-02 OK576278 Using Different Agricultural Wastes
description	Polyhydroxybutyrates (PHBs) are macromolecules synthesized by bacteria. Because of their fast degradability under natural environmental conditions, PHBs were selected as alternatives for the production of biodegradable plastics. Sixteen PHB-accumulating strains were selected and compared for their ability to accumulate PHB granules inside their cells. Isolate AS-02 was isolated from cattle manure and identified as <i>Bacillus wiedmannii</i> AS-02 OK576278 by means of 16S rRNA analysis. It was found to be the best producer. The optimum pH, temperature, and incubation period for the best PHB production by the isolate were 7, 35 °C, and 72 h respectively. PHB production was the best with peptone and glucose as nitrogen and carbon sources at a C/N ratio of (2:1). The strain was able to accumulate 423, 390, 249, 158, and 144 mg/L PHB when pretreated orange, mango, banana, onion peels, and rice straw were used as carbon sources, respectively. The extracted polymer was characterized by Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR), and GC-MS spectroscopy, which confirmed the structure of the polymer as PHB. The isolate <i>B. wiedmannii</i> AS-02 OK576278 can be considered an excellent candidate for industrial production of PHB from agricultural wastes.
format	article
author	Amal W. Danial Shereen M. Hamdy Sulaiman A. Alrumman Sanaa M. F. Gad El-Rab Ahmed A. M. Shoreit Abd El-Latif Hesham
author_facet	Amal W. Danial Shereen M. Hamdy Sulaiman A. Alrumman Sanaa M. F. Gad El-Rab Ahmed A. M. Shoreit Abd El-Latif Hesham
author_sort	Amal W. Danial
title	Bioplastic Production by <i>Bacillus wiedmannii</i> AS-02 OK576278 Using Different Agricultural Wastes
title_short	Bioplastic Production by <i>Bacillus wiedmannii</i> AS-02 OK576278 Using Different Agricultural Wastes
title_full	Bioplastic Production by <i>Bacillus wiedmannii</i> AS-02 OK576278 Using Different Agricultural Wastes
title_fullStr	Bioplastic Production by <i>Bacillus wiedmannii</i> AS-02 OK576278 Using Different Agricultural Wastes
title_full_unstemmed	Bioplastic Production by <i>Bacillus wiedmannii</i> AS-02 OK576278 Using Different Agricultural Wastes
title_sort	bioplastic production by <i>bacillus wiedmannii</i> as-02 ok576278 using different agricultural wastes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c226598ee9e14bd2807f9f7fb1eab5f7
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_version_	1718411222205857792

Bioplastic Production by <i>Bacillus wiedmannii</i> AS-02 OK576278 Using Different Agricultural Wastes

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