miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p

Abstract microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets...

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Autores principales: Cristina Gutiérrez-Vázquez, Ana Rodríguez-Galán, Marcos Fernández-Alfara, María Mittelbrunn, Fátima Sánchez-Cabo, Dannys Jorge Martínez-Herrera, Marta Ramírez-Huesca, Alberto Pascual-Montano, Francisco Sánchez-Madrid
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/c2274d1f5a744b7cac98049a132aa4fe
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Sumario:Abstract microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3′UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells.