miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p

Abstract microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets...

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Autores principales:	Cristina Gutiérrez-Vázquez, Ana Rodríguez-Galán, Marcos Fernández-Alfara, María Mittelbrunn, Fátima Sánchez-Cabo, Dannys Jorge Martínez-Herrera, Marta Ramírez-Huesca, Alberto Pascual-Montano, Francisco Sánchez-Madrid
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c2274d1f5a744b7cac98049a132aa4fe
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id	oai:doaj.org-article:c2274d1f5a744b7cac98049a132aa4fe
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spelling	oai:doaj.org-article:c2274d1f5a744b7cac98049a132aa4fe2021-12-02T12:31:54ZmiRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p10.1038/s41598-017-03689-72045-2322https://doaj.org/article/c2274d1f5a744b7cac98049a132aa4fe2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03689-7https://doaj.org/toc/2045-2322Abstract microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3′UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells.Cristina Gutiérrez-VázquezAna Rodríguez-GalánMarcos Fernández-AlfaraMaría MittelbrunnFátima Sánchez-CaboDannys Jorge Martínez-HerreraMarta Ramírez-HuescaAlberto Pascual-MontanoFrancisco Sánchez-MadridNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cristina Gutiérrez-Vázquez Ana Rodríguez-Galán Marcos Fernández-Alfara María Mittelbrunn Fátima Sánchez-Cabo Dannys Jorge Martínez-Herrera Marta Ramírez-Huesca Alberto Pascual-Montano Francisco Sánchez-Madrid miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p
description	Abstract microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3′UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells.
format	article
author	Cristina Gutiérrez-Vázquez Ana Rodríguez-Galán Marcos Fernández-Alfara María Mittelbrunn Fátima Sánchez-Cabo Dannys Jorge Martínez-Herrera Marta Ramírez-Huesca Alberto Pascual-Montano Francisco Sánchez-Madrid
author_facet	Cristina Gutiérrez-Vázquez Ana Rodríguez-Galán Marcos Fernández-Alfara María Mittelbrunn Fátima Sánchez-Cabo Dannys Jorge Martínez-Herrera Marta Ramírez-Huesca Alberto Pascual-Montano Francisco Sánchez-Madrid
author_sort	Cristina Gutiérrez-Vázquez
title	miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p
title_short	miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p
title_full	miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p
title_fullStr	miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p
title_full_unstemmed	miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p
title_sort	mirna profiling during antigen-dependent t cell activation: a role for mir-132-3p
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c2274d1f5a744b7cac98049a132aa4fe
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miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p

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