Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2

Summary: Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs,...

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Autores principales:	Hector Huerga Encabo, Rachel Ulferts, Aneesh Sharma, Rupert Beale, Dominique Bonnet
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell Biology Cell isolation Cell-based Assays Immunology Microbiology Microscopy Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/c23222a5106946579e969b824591faf8
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spelling	oai:doaj.org-article:c23222a5106946579e969b824591faf82021-11-14T04:35:36ZInfecting human hematopoietic stem and progenitor cells with SARS-CoV-22666-166710.1016/j.xpro.2021.100903https://doaj.org/article/c23222a5106946579e969b824591faf82021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006092https://doaj.org/toc/2666-1667Summary: Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay.For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021)Hector Huerga EncaboRachel UlfertsAneesh SharmaRupert BealeDominique BonnetElsevierarticleCell BiologyCell isolationCell-based AssaysImmunologyMicrobiologyMicroscopyScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100903- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Cell isolation Cell-based Assays Immunology Microbiology Microscopy Science (General) Q1-390
spellingShingle	Cell Biology Cell isolation Cell-based Assays Immunology Microbiology Microscopy Science (General) Q1-390 Hector Huerga Encabo Rachel Ulferts Aneesh Sharma Rupert Beale Dominique Bonnet Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2
description	Summary: Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay.For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021)
format	article
author	Hector Huerga Encabo Rachel Ulferts Aneesh Sharma Rupert Beale Dominique Bonnet
author_facet	Hector Huerga Encabo Rachel Ulferts Aneesh Sharma Rupert Beale Dominique Bonnet
author_sort	Hector Huerga Encabo
title	Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2
title_short	Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2
title_full	Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2
title_fullStr	Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2
title_full_unstemmed	Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2
title_sort	infecting human hematopoietic stem and progenitor cells with sars-cov-2
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c23222a5106946579e969b824591faf8
work_keys_str_mv	AT hectorhuergaencabo infectinghumanhematopoieticstemandprogenitorcellswithsarscov2 AT rachelulferts infectinghumanhematopoieticstemandprogenitorcellswithsarscov2 AT aneeshsharma infectinghumanhematopoieticstemandprogenitorcellswithsarscov2 AT rupertbeale infectinghumanhematopoieticstemandprogenitorcellswithsarscov2 AT dominiquebonnet infectinghumanhematopoieticstemandprogenitorcellswithsarscov2
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Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2

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