Fabrication, optimization, and characterization of umbelliferone β-D-galactopyranoside-loaded PLGA nanoparticles in treatment of hepatocellular carcinoma: in vitro and in vivo studies
Vikas Kumar,1 Prakash Chandra Bhatt,2 Mahfoozur Rahman,1 Gaurav Kaithwas,3 Hani Choudhry,4,5 Fahad A Al-Abbasi,4 Firoz Anwar,4 Amita Verma6 1Natural Product Drug Discovery Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Tech...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/c23550d73b344f8c8f0b95b8d5c764f9 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:c23550d73b344f8c8f0b95b8d5c764f9 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:c23550d73b344f8c8f0b95b8d5c764f92021-12-02T01:32:49ZFabrication, optimization, and characterization of umbelliferone β-D-galactopyranoside-loaded PLGA nanoparticles in treatment of hepatocellular carcinoma: in vitro and in vivo studies1178-2013https://doaj.org/article/c23550d73b344f8c8f0b95b8d5c764f92017-09-01T00:00:00Zhttps://www.dovepress.com/fabrication-optimization-and-characterization-of-umbelliferone-beta-d--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Vikas Kumar,1 Prakash Chandra Bhatt,2 Mahfoozur Rahman,1 Gaurav Kaithwas,3 Hani Choudhry,4,5 Fahad A Al-Abbasi,4 Firoz Anwar,4 Amita Verma6 1Natural Product Drug Discovery Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad, Uttar Pradesh, India; 2Centre for Advanced Research in Pharmaceutical Sciences, Microbial and Pharmaceutical Biotechnology Laboratory, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India; 3Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (Central University), Vidya Vihar, Rai Bareli Road, Lucknow, India; 4Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia; 5Cancer Metabolism and Epigenetic Unit, King Fahd Center for Medical Research, King Abdulaziz University, Jeddah, Saudi Arabia; 6Bio-organic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad, Uttar Pradesh, India Abstract: Umbelliferone β-D-galactopyranoside (UFG), isolated from plants, exhibits promising inhibitory action on numerous diseases. The present research was initiated to develop a suitable delivery system for UFG with an intention to enhance its therapeutic efficacy against diethyl nitrosamine (DEN)-induced hepatocellular carcinoma (HCC) in Wistar rats. UFG-loaded polymeric nanoparticles prepared by sonication were scrutinized for average size, drug loading capacity, zeta potential, and drug release potency in animals. HCC cell lines HuH-7 and Hep G2 were used for in vitro cytotoxic investigation. Several hepatic, nonhepatic, antioxidant, and anti-inflammatory biochemical parameters were estimated to establish the anticancer potential of UFG nanoformulation. Microscopical and histopathological investigations were also undertaken to substantiate the results of our work. Umbelliferone β-D-galactopyranoside-loaded poly(d,l-lactide-co-glycolide) nanoparticles (UFG-PLGA-NP) with particle size of 187.1 nm and polydispersity index 0.16 were uniform in nature with 82.5% release of the total amount of drug after 48 h. Our study successfully established the development and characterization of UFG-PLGA-NP with noticeable effect against both in vivo and in vitro models. The anticancer potential of UFG-PLGA-NP was brought about by the management of DEN-induced reactive oxygen species generation, mitochondrial dysfunction, proinflammatory cytokines alteration, and induction of apoptosis. Positive zeta potential on the surface of UFG-PLGA-NP would have possibly offered higher hepatic accumulation of UFG, particularly in the electron-dense mitochondria organelles, and this was the take-home message from this study. Our results demonstrated that such polymer-loaded delivery systems of UFG can be a better option and can be further explored to improve the clinical outcomes against hepatic cancer. Keywords: hepatocellular carcinoma, umbelliferone β-D-galactopyranoside nanoparticles, diethyl nitrosamine, cytokines, oxidative stress Kumar VBhatt PCRahman MKaithwas GChoudhry HAl-Abbasi FAAnwar FVerma ADove Medical PressarticleHepatocellular carcinomaUmbelliferone β-D-galactopyranoside nanoparticlesdiethyl nitrosaminecytokinesoxidative stress.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 6747-6758 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Hepatocellular carcinoma Umbelliferone β-D-galactopyranoside nanoparticles diethyl nitrosamine cytokines oxidative stress. Medicine (General) R5-920 |
| spellingShingle |
Hepatocellular carcinoma Umbelliferone β-D-galactopyranoside nanoparticles diethyl nitrosamine cytokines oxidative stress. Medicine (General) R5-920 Kumar V Bhatt PC Rahman M Kaithwas G Choudhry H Al-Abbasi FA Anwar F Verma A Fabrication, optimization, and characterization of umbelliferone β-D-galactopyranoside-loaded PLGA nanoparticles in treatment of hepatocellular carcinoma: in vitro and in vivo studies |
| description |
Vikas Kumar,1 Prakash Chandra Bhatt,2 Mahfoozur Rahman,1 Gaurav Kaithwas,3 Hani Choudhry,4,5 Fahad A Al-Abbasi,4 Firoz Anwar,4 Amita Verma6 1Natural Product Drug Discovery Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad, Uttar Pradesh, India; 2Centre for Advanced Research in Pharmaceutical Sciences, Microbial and Pharmaceutical Biotechnology Laboratory, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India; 3Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (Central University), Vidya Vihar, Rai Bareli Road, Lucknow, India; 4Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia; 5Cancer Metabolism and Epigenetic Unit, King Fahd Center for Medical Research, King Abdulaziz University, Jeddah, Saudi Arabia; 6Bio-organic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad, Uttar Pradesh, India Abstract: Umbelliferone β-D-galactopyranoside (UFG), isolated from plants, exhibits promising inhibitory action on numerous diseases. The present research was initiated to develop a suitable delivery system for UFG with an intention to enhance its therapeutic efficacy against diethyl nitrosamine (DEN)-induced hepatocellular carcinoma (HCC) in Wistar rats. UFG-loaded polymeric nanoparticles prepared by sonication were scrutinized for average size, drug loading capacity, zeta potential, and drug release potency in animals. HCC cell lines HuH-7 and Hep G2 were used for in vitro cytotoxic investigation. Several hepatic, nonhepatic, antioxidant, and anti-inflammatory biochemical parameters were estimated to establish the anticancer potential of UFG nanoformulation. Microscopical and histopathological investigations were also undertaken to substantiate the results of our work. Umbelliferone β-D-galactopyranoside-loaded poly(d,l-lactide-co-glycolide) nanoparticles (UFG-PLGA-NP) with particle size of 187.1 nm and polydispersity index 0.16 were uniform in nature with 82.5% release of the total amount of drug after 48 h. Our study successfully established the development and characterization of UFG-PLGA-NP with noticeable effect against both in vivo and in vitro models. The anticancer potential of UFG-PLGA-NP was brought about by the management of DEN-induced reactive oxygen species generation, mitochondrial dysfunction, proinflammatory cytokines alteration, and induction of apoptosis. Positive zeta potential on the surface of UFG-PLGA-NP would have possibly offered higher hepatic accumulation of UFG, particularly in the electron-dense mitochondria organelles, and this was the take-home message from this study. Our results demonstrated that such polymer-loaded delivery systems of UFG can be a better option and can be further explored to improve the clinical outcomes against hepatic cancer. Keywords: hepatocellular carcinoma, umbelliferone β-D-galactopyranoside nanoparticles, diethyl nitrosamine, cytokines, oxidative stress |
| format |
article |
| author |
Kumar V Bhatt PC Rahman M Kaithwas G Choudhry H Al-Abbasi FA Anwar F Verma A |
| author_facet |
Kumar V Bhatt PC Rahman M Kaithwas G Choudhry H Al-Abbasi FA Anwar F Verma A |
| author_sort |
Kumar V |
| title |
Fabrication, optimization, and characterization of umbelliferone β-D-galactopyranoside-loaded PLGA nanoparticles in treatment of hepatocellular carcinoma: in vitro and in vivo studies |
| title_short |
Fabrication, optimization, and characterization of umbelliferone β-D-galactopyranoside-loaded PLGA nanoparticles in treatment of hepatocellular carcinoma: in vitro and in vivo studies |
| title_full |
Fabrication, optimization, and characterization of umbelliferone β-D-galactopyranoside-loaded PLGA nanoparticles in treatment of hepatocellular carcinoma: in vitro and in vivo studies |
| title_fullStr |
Fabrication, optimization, and characterization of umbelliferone β-D-galactopyranoside-loaded PLGA nanoparticles in treatment of hepatocellular carcinoma: in vitro and in vivo studies |
| title_full_unstemmed |
Fabrication, optimization, and characterization of umbelliferone β-D-galactopyranoside-loaded PLGA nanoparticles in treatment of hepatocellular carcinoma: in vitro and in vivo studies |
| title_sort |
fabrication, optimization, and characterization of umbelliferone β-d-galactopyranoside-loaded plga nanoparticles in treatment of hepatocellular carcinoma: in vitro and in vivo studies |
| publisher |
Dove Medical Press |
| publishDate |
2017 |
| url |
https://doaj.org/article/c23550d73b344f8c8f0b95b8d5c764f9 |
| work_keys_str_mv |
AT kumarv fabricationoptimizationandcharacterizationofumbelliferonebetadgalactopyranosideloadedplgananoparticlesintreatmentofhepatocellularcarcinomainvitroandinvivostudies AT bhattpc fabricationoptimizationandcharacterizationofumbelliferonebetadgalactopyranosideloadedplgananoparticlesintreatmentofhepatocellularcarcinomainvitroandinvivostudies AT rahmanm fabricationoptimizationandcharacterizationofumbelliferonebetadgalactopyranosideloadedplgananoparticlesintreatmentofhepatocellularcarcinomainvitroandinvivostudies AT kaithwasg fabricationoptimizationandcharacterizationofumbelliferonebetadgalactopyranosideloadedplgananoparticlesintreatmentofhepatocellularcarcinomainvitroandinvivostudies AT choudhryh fabricationoptimizationandcharacterizationofumbelliferonebetadgalactopyranosideloadedplgananoparticlesintreatmentofhepatocellularcarcinomainvitroandinvivostudies AT alabbasifa fabricationoptimizationandcharacterizationofumbelliferonebetadgalactopyranosideloadedplgananoparticlesintreatmentofhepatocellularcarcinomainvitroandinvivostudies AT anwarf fabricationoptimizationandcharacterizationofumbelliferonebetadgalactopyranosideloadedplgananoparticlesintreatmentofhepatocellularcarcinomainvitroandinvivostudies AT vermaa fabricationoptimizationandcharacterizationofumbelliferonebetadgalactopyranosideloadedplgananoparticlesintreatmentofhepatocellularcarcinomainvitroandinvivostudies |
| _version_ |
1718403038189715456 |